Function of 3' UTRs

3 UTR 的功能

基本信息

批准号：
8604714
负责人：
Marvin P. Wickens
金额：
$ 46.65万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-04-01 至 2016-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8604714
关键词：
3&apos Untranslated Regions Affinity Architecture Binding Binding Sites Biochemical Biochemistry Biological Biology Caenorhabditis elegans Cell Aging Cells Clinical Collaborations Complex Critical Pathways DNA Development Developmental Biology Diagnosis Elements Equilibrium Event Evolution Family Fertility Genetic Genetic Translation Goals Homeostasis Human Intervention Location Logic Mediating Memory Messenger RNA Methods Molecular Molecular Genetics Movement Orthologous Gene Physiological Physiology Poly(A) Tail Process Protein Binding Proteins RNA RNA Binding RNA Sequences Repression Research Role Saccharomyces cerevisiae Specificity Stem cells Terminator Codon Testing Translations Untranslated Regions Work Xenopus Xenopus oocyte Yeasts design fly genetic regulatory protein in vivo insight interest mRNA Stability novel strategies public health relevance research study scaffold yeast protein

项目摘要

DESCRIPTION (provided by applicant): DNA makes RNA makes protein. The second step of that pathway - the critical function of RNA in genetic information transfer - is exquisitely regulated. mRNA activity, stability and location are controlled by factors that recognize specific RNA sequences. The PUF proteins are a widespread family of mRNA regulatory proteins that control key steps in early development and are required for establishing memory. They must find and recognize specific mRNAs, and then execute that mRNA's fate - activation, repression, destruction, or movement. We elucidate the way in which these proteins form networks of control - recognizing and controlling a substantial proportion of the mRNAs in human cells. They act through collaborations with protein partners - interactions that are conserved from yeast to humans. PUF proteins and their partners have important roles in development, homeostasis, cellular senescence, and human fertility. The networks of RNAs they control are vital in these processes.

描述（由申请人提供）：DNA 制造 RNA 制造蛋白质。该途径的第二步——RNA 在遗传信息传递中的关键功能——受到精心调控。 mRNA 的活性、稳定性和位置由识别特定 RNA 序列的因素控制。 PUF 蛋白是一个广泛分布的 mRNA 调节蛋白家族，控制着早期发育的关键步骤，并且是建立记忆所必需的。他们必须找到并识别特定的 mRNA，然后执行该 mRNA 的命运——激活、抑制、破坏或移动。我们阐明了这些蛋白质形成控制网络的方式——识别和控制人类细胞中大部分的 mRNA。它们通过与蛋白质伙伴的合作来发挥作用——这种相互作用从酵母到人类都是保守的。 PUF 蛋白及其伙伴在发育、体内平衡、细胞衰老和人类生育能力中发挥着重要作用。它们控制的 RNA 网络在这些过程中至关重要。