ESR STUDY OF IRON COMPOUNDS FOR UNDERSTANDING NITROGEN FIXATION

用于了解固氮作用的铁化合物的 ESR 研究

• 批准号: 8364086
• 负责人: BORIS G DZIKOVSKI
• 金额: $ 0.05万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364086
• 关键词: Chemistry; Enzymes; Funding; Grant; Helium; Investigation; Iron Compounds; Liquid substance; Molybdoferredoxin; National Center for Research Resources; Nitrogen Fixation; Nitrogenase; Organic Iron Compounds; Principal Investigator; Relaxation; Research; Research Infrastructure; Resources; Source; Spectrum Analysis; Sulfides; Technology; Time; United States National Institutes of Health; Universities; cost; cryostat; instrumentation; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Holland group from the University of Rochester's Department of Chemistry utilized the X-Band EPR instrumentation at ACERT to study iron compounds with hydride and sulfide donors. These studies were part of an on-going investigation of the spectroscopy of coordination compounds with the same coordination features as the iron-molybdenum cofactor of nitrogenase. These are intended to facilitate the use of spectroscopy to understand the enzyme. The continuous-flow, liquid helium cryostat available at ACERT is essential for these experiments because the EPR spectra of cyts c are typically not observed above 30 K due to a relatively short spin-lattice relaxation time.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 罗切斯特大学化学系的 Holland 小组利用 ACERT 的 X 波段 EPR 仪器研究具有氢化物和硫化物供体的铁化合物。这些研究是正在进行的配位化合物光谱研究的一部分，该配位化合物具有与固氮酶铁钼辅因子相同的配位特征。这些旨在促进使用光谱学来了解酶。 ACERT 提供的连续流液氦低温恒温器对于这些实验至关重要，因为由于自旋晶格弛豫时间相对较短，通常在 30 K 以上观察不到细胞色素 c 的 EPR 光谱。