CHEMISTRY, NEUROBIOLOGY, AND MASS SPECTROMETRY

化学、神经生物学和质谱

• 批准号: 8361383
• 负责人: JOSHUA MAUER
• 金额: $ 0.11万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361383
• 关键词: Address; Aluminum Oxide; Biochemistry; Biological; Biosensor; Brain; Cells; Cellular biology; Chemicals; Chemistry; Complex; Cues; Development; Funding; Gold; Grant; Human; Ion Channel; Mass Spectrum Analysis; Methods; Modeling; Molecular Biology; National Center for Research Resources; Neurobiology; Neuronal Differentiation; Neurons; Organic Chemistry; Pattern; Photochemistry; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Signal Transduction; Source; Surface; Techniques; United States National Institutes of Health; biomedical resource; cost; insight; interest; monolayer; neuronal guidance; small molecule; titanium dioxide; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Mauer group is interested in using chemical methods to understand problems in neurobiology and development, as well as exploiting neurobiology to construct biosensors. In general, answers to the biological questions addressed by the group are not accessible using traditional biological techniques. As a result, the group makes use of chemical approaches and a variety of tools to gain new insights into complex biological problems. The tools we use to address these problems include synthetic organic chemistry, surface chemistry, biochemistry, molecular biology, and cell biology. The Mauer group is specifically interested in Ion-Channel Biosensors, Neuronal Differentiation, and Neuronal Wiring and Development. Human brains consist of an enormous number of neurons that interact with each other in very specific and complex ways to give rise to human thought and function. Neurons within this network are guided to make specific connections through a variety of chemical signals that include small molecules, soluble proteins, and proteins presented on the surface of other cells. The Mauer lab is interested in developing minimal models to understand neuronal guidance by spatially patterning guidance cues. Patterning is achieved through the creation of photopatternable self-assembled monolayers (SAMs) on gold, aluminum oxide, and titanium oxide. The use of organic photochemistry allows multiple proteins to be easily presented on a single culture surface. Our chemical approach to this problem allows construction of complex model neuronal networks that cannot be achieved using traditional methods.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 莫尔小组有兴趣使用化学方法来理解神经生物学和发育问题，以及利用神经生物学构建生物传感器。一般来说，使用传统的生物学技术无法获得该小组所解决的生物学问题的答案。因此，该小组利用化学方法和各种工具来获得对复杂生物问题的新见解。我们用来解决这些问题的工具包括合成有机化学、表面化学、生物化学、分子生物学和细胞生物学。 Mauer 小组对离子通道生物传感器、神经元分化以及神经元接线和发育特别感兴趣。人类大脑由大量神经元组成，这些神经元以非常特定和复杂的方式相互作用，从而产生人类的思想和功能。该网络内的神经元被引导通过各种化学信号建立特定的连接，这些化学信号包括小分子、可溶性蛋白质和其他细胞表面存在的蛋白质。莫尔实验室有兴趣开发最小模型，通过空间图案引导线索来理解神经元的引导。图案化是通过在金、氧化铝和氧化钛上创建可光图案化的自组装单层 (SAM) 来实现的。有机光化学的使用使得多种蛋白质可以轻松地呈现在单个培养物表面上。我们解决这个问题的化学方法允许构建使用传统方法无法实现的复杂模型神经元网络。