NIR ENZYME-ACTIVATED FLUOROGENIC PHOSPHOLIPIDS FOR MULTI-MODALITY IMAGING

用于多模态成像的近红外酶激活荧光磷脂

• 批准号: 8361955
• 负责人: EDWARD J DELIKATNY
• 金额: $ 0.77万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361955
• 关键词: Diagnostic; Disease Management; Early Diagnosis; Enzymes; Funding; Goals; Grant; Hydrolysis; Lead; Lipids; Magnetic Resonance; Magnetic Resonance Spectroscopy; Malignant Neoplasms; Membrane; Methods; Molecular Probes; National Center for Research Resources; Pathologic Processes; Phospholipase; Phospholipid Metabolism; Phospholipids; Physiological Processes; Principal Investigator; Research; Research Infrastructure; Resources; Second Messenger Systems; Signal Pathway; Signal Transduction; Source; United States National Institutes of Health; chemotherapy; cost; imaging modality; improved; in vivo; molecular imaging; novel; optical imaging; second messenger

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Phospholipases catalyze the hydrolysis of membrane phospholipids, which, in turn, generates a variety of lipid second messengers. These lipid-derived products are necessary for both physiological and pathological processes. An understanding of phospholipid metabolism, as it relates to cancer, is necessary for diagnostic applications and for developing novel targeted chemotherapy. Traditionally, lipid metabolite changes have been observed by magnetic resonance spectroscopy (MRS) or by chromatographic methods. Using a combination of MRS with near-infrared (NIR) fluorescent molecular probes we are targeting phospholipase function with the goal of delineating specific cell signaling pathways in vivo. The information gained from a molecular imaging approach may lead to earlier diagnoses and improved management of disease.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 磷脂酶催化膜磷脂的水解，进而产生 多种脂质第二信使。这些脂质衍生产品对于两者都是必需的 生理和病理过程。了解磷脂代谢，因为它 与癌症相关，对于诊断应用和开发新的靶向药物是必要的 化疗。传统上，脂质代谢变化是通过磁力观察的。 共振光谱（MRS）或色谱方法。使用组合 带有近红外 (NIR) 荧光分子探针的 MRS，我们针对的是磷脂酶 功能的目的是描绘体内特定的细胞信号传导途径。信息 从分子成像方法获得的信息可能会导致早期诊断并改善 疾病管理。