Tomographic Fluorescent and Bioluminescent Optical Imaging

断层荧光和生物发光光学成像

• 批准号: 8247267
• 负责人: EDWARD J DELIKATNY
• 金额: $ 38.03万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247267
• 关键词: Algorithms; Animals; Atlases; Biological Sciences; Core Facility; Custom; Diffuse; Fluorescence; Funding; Image; Lighting; Mus; Operative Surgical Procedures; Pennsylvania; Positron-Emission Tomography; Reporter; Request for Applications; Signal Transduction; Silicon Dioxide; Small Animal Imaging Systems; Source; System; Tissues; Ultrasonography; Universities; digital; fluorescence imaging; in vivo; instrument; lens; optical imaging; pre-clinical research; quantum; square foot; tomography; tool

DESCRIPTION (provided by applicant): This application requests funds for an IVIS Spectrum, an in vivo small animal imaging system for preclinical research use manufactured by Caliper Life Sciences (Hopkinton, MA). The IVIS Spectrum is an advanced in vivo imaging system for imaging of deep tissue bioluminescent and fluorescent sources in whole animals. It features an optimized set of high efficiency filters and spectral un-mixing algorithms that allow imaging of bioluminescent and fluorescent reporters across the blue to near infrared wavelengths. It also offers single- view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using a Digital Mouse Atlas. The IVIS Spectrum incorporates a custom fused silica 1 inch CCD with a FOV between 3.5 cm and 25 cm, a quantum efficiency of 85% between 500-700nm and a true f/1 lens. The combination of these specifications provides for highly sensitive bioluminescent imaging with a dynamic range of 11 orders of magnitude. For fluorescence imaging, the IVIS Spectrum can use either trans-illumination or epi-illumination to illuminate in vivo fluorescent sources. This capability allows 3D diffuse fluorescence tomography such that both source localization and concentration can be determined. The instrument is equipped with narrow band excitation and emission filters that assist in significantly reducing autofluorescence. In combination with the spectral unmixing tools, this allows the separation of signals from multiple fluorescent reporters within the same animal. The IVIS Spectrum will be available to multiple users as part of the Small Animal Imaging Facility (SAIF) at the University of Pennsylvania. It will be placed in the newly renovated 2,100 sq ft. Core Facility adjacent to the 9.4 T small animal MR, micro-PET and CT, and animal ultrasound systems, adjacent to a 400 cage animal vivarium with advanced animal handling and surgery rooms. The IVIS Spectrum will add considerable functionality to the SAIF at the University of Pennsylvania. PUBLIC HEALTH RELEVANCE: This application requests funds for an IVIS Spectrum system for preclinical optical imaging of mice. The system is unique in that it can provide three-dimensional bioluminescence and fluorescence images in a format that is easily co-registered with images from other modalities. The system will be located adjacent to existing small animal MRI and ultrasound scanners and used to develop and enhance multi-modality imaging in mouse models of human disease.

描述（由申请人提供）：本申请要求为 IVIS Spectrum 提供资金，这是一种由 Caliper Life Sciences（马萨诸塞州霍普金顿）制造的用于临床前研究的体内小动物成像系统。 IVIS Spectrum 是一种先进的体内成像系统，用于对整个动物的深层组织生物发光和荧光源进行成像。它具有一组优化的高效滤光片和光谱解混合算法，允许在蓝色到近红外波长范围内对生物发光和荧光报告基因进行成像。它还为荧光和生物发光记者提供单视图 3D 断层扫描，可以使用数字鼠标图谱在解剖环境中进行分析。 IVIS Spectrum 采用定制的熔融石英 1 英寸 CCD，FOV 在 3.5 cm 至 25 cm 之间，500-700 nm 之间的量子效率为 85%，并且配备真正的 f/1 透镜。这些规格的组合提供了动态范围为 11 个数量级的高灵敏度生物发光成像。对于荧光成像，IVIS Spectrum 可以使用透射照明或落射照明来照亮体内荧光源。此功能允许进行 3D 漫射荧光断层扫描，从而可以确定源定位和浓度。该仪器配备了窄带激发和发射滤光片，有助于显着减少自发荧光。与光谱分离工具结合使用，可以分离同一动物内多个荧光报告基因的信号。 IVIS Spectrum 将作为宾夕法尼亚大学小动物成像设施 (SAIF) 的一部分向多个用户开放。它将放置在新装修的 2,100 平方英尺核心设施中，毗邻 9.4 吨小动物 MR、微型 PET 和 CT 以及动物超声系统，毗邻拥有先进动物处理和手术室的 400 个笼子动物饲养场。 IVIS Spectrum 将为宾夕法尼亚大学 SAIF 添加大量功能。 公共健康相关性：该申请要求为用于小鼠临床前光学成像的 IVIS Spectrum 系统提供资金。该系统的独特之处在于它可以提供三维生物发光和荧光图像，其格式很容易与其他模式的图像共同配准。该系统将毗邻现有的小动物 MRI 和超声波扫描仪，用于开发和增强人类疾病小鼠模型的多模态成像。