A Comparative Study of M. tuberculosis and M. bovis BCG of T cell responses

结核分枝杆菌和牛分枝杆菌卡介苗T细胞反应的比较研究

• 批准号: 8677401
• 负责人: Patricia Grace
• 金额: $ 4.27万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-03-01 至 2016-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8677401
• 关键词: Address; Aerosols; Animal Housing; Animals; Antigen Presentation; Antigen-Presenting Cells; Antigens; Award; Bacteria; Behavior; Biology; CD4 Positive T Lymphocytes; CD8B1 gene; Cataloging; Catalogs; Cell Communication; Cells; Characteristics; Clinical; Comparative Study; Cultured Cells; Data; Dendritic Cells; Development; Disease; Dry Ice; Elevator; Environmental air flow; Floods; Frequencies; Goals; HIV; Health; Histocompatibility Antigens Class II; House mice; Housing; Human; Hurricane; Image; Immune; Immune response; Immunity; Immunocompetent; Immunology; In Vitro; Infection; Inferior; Laboratories; Life; Location; Lung; Mechanics; Medical center; Methods; Mus; Mycobacterium tuberculosis; Pharmaceutical Preparations; Plumbing; Process; Public Health; Publications; Reagent; Recording of previous events; Reporting; Research; Running; Safety; Sampling; Site; Sterility; T cell response; T-Lymphocyte; Techniques; Testing; Time; Training; Tuberculosis; Tuberculosis Vaccines; Update; Virulent; Water; Work; adaptive immunity; base; biomedical scientist; biosafety level 3 facility; career; in vivo; indexing; innovation; macrophage; prevent; public health research; research study; residence; tissue culture; tuberculosis immunity

On October 29, 2012, the NYU Langone Medical Center was hit by the Superstorm Sandy, which caused significant damages to the facilities used to conduct my research. Hurricane Sandy caused storm surges that exceeded what our facilities could withstand, leading to significant flooding. The repercussions of this flooding led to mechanical, electrical, and plumbing damages in many of the research buildings at NYU. These damages led to immediate loss of experiments and reagents but have also long-term delays in the completion of this project. In the wake of Hurricane Sandy, access to all research buildings was limited due to health and safety issues. Smilow Research Building, our main laboratory location, had no power for a week post-Sandy, which meant that freezers and refrigerators containing reagents we use routinely for experimentation were lost. Full-power and unrestricted access to the Smilow Research Building was not restored until one month post-Sandy. After the re-opening of Smilow, an additional 2 months of our time was dedicated to cataloging research losses and purchasing new reagents for experimentation. The Berg Building, which houses our Biosafety Level 3 (ABSL3) facility, remains without power. This facility housed animals infected with M. tuberculosis (M.tb) and is where we processed all infected samples prior to the storm. All infectious samples from on-going experiments were stored in refrigerators and freezers within the ABSL3 facility. We were able to save bacterial strains stored within our -80C freezer by supplying it with dry ice, however many other samples from on-going experiments could not be saved. The loss of these experimental samples is equal to a loss of three 6-week experiments. Work with the infected animals of the ongoing experiments at that time was significantly delayed in the absence of a proper ABSL3 facility and prevented the analysis of samples at key time points during infection. The ABSL3 located in Berg remains without power, ventilation, running water, or elevator access; the reopening of this facility is projected to be in September of this year, which will make it nearly a year post-Sandy, that this facility has been shut down. In the meantime we have made use of a second BSL3 facility within the Smilow Research Building, which is now operational on a limited basis to resume M. tuberculosis experiments. This facility was suited for in vitro work, with one Tissue Culture (TC) hood available for TB work, which was a huge bottleneck for progress, since amongst the TB labs at NYU we went from 4 available TC hoods to 1 available hood for experimentation. With the exception of Aim 1 of this project, all other proposed experiments for this award require mouse infections with M. tuberculosis. In April 2013, 6 months post-Sandy, we were able to expand to 2 TC hoods (shared by 15 people), it was also at this time that we were able to relocate the aerosol unit from our ABSL3 in the Berg Building to this Smilow BSL3. A satellite mouse housing facility has been built to accommodate our need for infected animal housing post-Sandy. To-date, we are ~20% operational post-Sandy.

2012年10月29日，NYU Langone医疗中心遭到超级风暴桑迪的袭击，这引起了 用于进行我的研究的设施的重大损害。桑迪飓风造成了暴风雨 超过了我们的设施可以承受的东西，导致洪水泛滥。这种洪水的影响 在纽约大学的许多研究建筑中导致机械，电气和管道损坏。这些 损坏导致立即损失实验和试剂，但在完成中也有长期的延误 这个项目。 飓风桑迪（Sandy）之后，由于健康和安全问题，进入所有研究建筑物都受到限制。 Smilow研究大楼是我们的主要实验室所在地，桑迪一周没有权力，这意味着 我们常规用于实验的冰柜和冰箱含有试剂。全功率 直到桑迪后一个月才恢复无限制进入Smilow研究大楼的机会。后 Smilow的重新开放，我们的2个月的时间致力于分类研究损失和 购买新试剂进行实验。 伯格大楼容纳我们的生物安全3级（ABSL3）设施，它仍然没有动力。这个设施 感染了结核分枝杆菌（M.TB）的动物，是我们在此之前处理所有感染样品的地方 暴风雨。正在进行的实验中的所有感染样品都存储在冰箱和冰柜中 ABSL3设施。我们能够通过供应干燥的细菌菌株来节省-80C冰箱中的细菌菌株 冰，但是许多来自正在进行的实验的样本无法保存。这些损失 实验样品等于三个6周实验的损失。与受感染的动物一起工作 当时正在进行的实验在没有适当的ABSL3设施和 防止感染过程中的关键时间点对样品进行分析。位于伯格保留的ABSL3 没有电力，通风，自来水或电梯通道；该设施的重新开放预计将在 今年9月，将在桑迪（Sandy）后将近一年的时间，该设施已被关闭。 同时，我们利用了Smilow研究大楼内的第二个BSL3设施，这是 现在，以有限的基础进行操作，以恢复结核分枝杆菌实验。该设施适合体外 工作，有一种组织培养（TC）罩可用于结核病工作，这是一个巨大的进度瓶颈， 由于在纽约大学的结核病实验室中，我们从4个可用的TC引擎盖到1个可用的引擎盖进行实验。 除本项目的AIM 1外，该奖项的所有其他提出的实验都需要鼠标 结核分枝杆菌感染。 2013年4月，桑迪后6个月，我们能够扩展到2个TC引擎盖 （由15个人共享），此时我们也能够从我们的Absl3中搬迁气溶胶单元 这款Smilow BSL3的Berg建筑物。已经建造了卫星鼠标外壳设施以适应我们的 桑迪后需要感染的动物住房。迄今为止，我们的运营后票房约为20％。