Kidney Imaging

肾脏影像

基本信息

批准号：
8734389
负责人：
Gerard L Apodaca
金额：
$ 24.64万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8734389
关键词：
Acute Renal Failure with Renal Papillary Necrosis Affect Affinity Animal Experimentation Antibodies Attention Binding Biology Bladder Cell Culture Techniques Cells Complement Data Disease Educational process of instructing Ensure Equipment Experimental Designs Familiarity Fluorochrome Functional disorder Funding Goals Gold Harvest Histology Human Resources Image Image Analysis Imaging Device Immunology Instruction Interdisciplinary Study Kidney Knowledge Label Learning Life Lower urinary tract Magnetic Resonance Imaging Methods Microscope Mission Molecular Biology Morphology Organ Pathology Physiology Postdoctoral Fellow Procedures Productivity Protocols documentation Quality Control Reagent Research Research Personnel Resolution Resources Secure Staining method Stains Students Techniques Tissue Fixation Tissues Training Transplantation United States National Institutes of Health Urinary tract base blood oxygen level dependent high standard imaging modality in vivo insight intravital microscopy kidney imaging light microscopy multidisciplinary novel protein distribution tissue fixing tissue/cell culture tool urinary urinary tract imaging

项目摘要

PROJECT SUMMARY (See instructions): The Kidney Imaging Core provides a national resource for Center users who require detailed and quantitative morphological analysis of kidney- and lower urinary tract-associated cells, tissues, and organs. The Imaging Core complements each of the other Center cores by providing critical information about the amounts, localization, and dynamics of molecules, cells, and tissues in normal and disease states. Thus, the Imaging Core is an integral part of the Center's mission to support multidisciplinary research in physiology, pathophysiology, and molecular biology of the kidney, as well as training and information transfer amongst Center users and cores. Users of the Imaging Core have access to state-of-the-art tools that allow for live cell and intravital microscopy, stimulated emission depletion (STED) super-resolution imaging, ultrastructural analysis, and novel magnetic resonance imaging modalities. Furthermore, the Imaging Core provides expertise that includes: detailed knowledge of kidney and lower urinary tract histology, pathology, and cytoarchitecture; sophisticated know-how in the proper techniques for culturing cells and tissues, as well as harvesting and fixing tissue obtained from research animals; familiarity with the specialized staining techniques needed to label these cells/tissues; expertise in the proper techniques to image and analyze live kidney and lower urinary tract tissues both ex vivo and in vivo; and knowledge of experimental design and image analysis, including interpretation of morphological data. The broad, long-range objectives and goals of the Core are multifold: (1) to serve as a national center for imaging of kidney- and lower urinary tractassociated cells, tissues, and organs; (2) to support the specialized techniques, methods, and procedures utilized by the investigators that comprise the Pittsburgh Center for Kidney Research; (3) to transfer Imaging Core expertise to Center investigators through training mechanisms including mini sabbaticals; (4) to provide for a higher standard of quality control through the Core's ability to conduct procedures on a routine and consistent basis.

项目摘要（请参阅说明）：肾脏成像核心为中心用户提供了国家资源，这些中心用户需要对肾脏和下尿路相关的细胞，组织和器官进行详细和定量的形态学分析。成像核心通过提供有关正常和疾病状态中分子，细胞和组织的数量，定位和动力学的关键信息，可以补充其他中心核心。因此，成像核心是该中心支持肾脏生理学，病理生理学和分子生物学多学科研究的任务的组成部分，以及中心用户和核心之间的培训和信息转移。成像核心的用户可以使用最先进的工具，这些工具允许实时细胞和插入式显微镜，刺激的发射消耗（STED）超分辨率成像，超微结构分析以及新颖的磁共振成像方式。此外，成像核心提供了专业知识，其中包括：肾脏的详细知识和较低的尿路组织学，病理学和细胞结构；在适当的培养细胞和组织的适当技术中，以及从研究动物那里获得的组织的精致技术；熟悉标记这些细胞/组织所需的专门染色技术；适当的技术专业知识，以形象和分析活体和体内的尿路组织和较低的尿路组织；以及实验设计和图像分析的知识，包括对形态数据的解释。核心的广泛，远程目标和目标是多重的：（1）作为肾脏和下尿液拖拉拖拉拖拉片的成像中心；（2）支持构成匹兹堡肾脏研究中心的研究人员使用的专业技术，方法和程序；（3）将成像核心专业知识传递给研究人员通过包括迷你休假的培训机制；（4）通过核心的常规和一致性进行程序的能力提供更高的质量控制标准。