Domestication and characterization of TM7-the most elusive oral phylum

TM7——最难以捉摸的口腔门的驯化和表征

• 批准号: 8612839
• 负责人: Xuesong He
• 金额: $ 43.23万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8612839
• 关键词: Adherence; Area; Attention; Bacteria; Behavior; Biological; Biological Assay; Cell Death; Cell Separation; Cells; Coculture Techniques; Communities; Complex; Culture Media; Disease; Environment; Epithelial Cells; Equipment and supply inventories; Gene Cluster; Gene Expression; Genes; Genetic; Genome; Genomics; Gingiva; Goals; Growth; Health; Human; Human Microbiome; Human body; In Vitro; Inflammatory; Inflammatory Bowel Diseases; Laboratories; Life; Lymphocyte; Mass Spectrum Analysis; Metabolic; Metabolic Pathway; Microbiology; Mining; Modeling; Molecular; Names; Oral; Oral cavity; Pathogenesis; Pathway interactions; Periodontal Diseases; Periodontitis; Phenotype; Physiological; Production; Research; Research Personnel; Resources; Role; Site; Techniques; Testing; Transcript; Tube; Veillonella atypica; Virulence; Work; base; chemokine; functional genomics; improved; interest; member; microbial; microorganism; novel; oral bacteria; pathogen; public health relevance; research study; response; small molecule; tissue/cell culture; transcriptome sequencing

Abstract: The human microbiome project has provided a comprehensive inventory of the microorganisms associated with the human body. The next challenge is to understand their role in human health and disease which requires cultivation of the species of interest. Relevant to this application, more than half of the oral microorganisms are categorized as uncultivated phylotypes including many associated with oral microbial pathogenesis. Among them, members of the candidate division TM7 phylum are the most enigmatic bacterial group in the oral cavity. Despite their ubiquitous occurrence in human body sites and other environments, no TM7 representative has been cultivated as pure culture. As a phylum containing suspected pathogens associated with inflammatory mucosal diseases, particularly periodontitis, cultivation of TM7 and the ability to investigate their pathogenic features has become like a "Holy Grail" in microbiology. Using a novel medium and enrichment techniques, we were able to reduce TM7-containing cultures from a complex community to dual-species co-cultures containing the disease associated TM7 phylotype (TM7x) together with Atopobium parvulum or Veillonella atypica as partner strain. We hypothesize that the growth of TM7x requires specific partner species and that understanding their relationship may be the key for isolating pure culture of TM7x. Furthermore, the present semi-pure cultures of TM7x and the possible pure TM7x cultures obtained through this study will enable our ability to study genomics, interactomics and the pathogenic potential of TM7x. This application aims to test our hypothesis and achieve these goals through detailed molecular analyses of the relationship between TM7x and its partner species, improving the medium for cultivating pure TM7x, as well as exploring its pathogenic potential. The immediate impact of the study is the discovery of cultivation conditions for TM7x and the associated biological/physiological functions. The long-term significance of the study is the creation of a new pathway for cultivation of many other yet-to-be cultured potentially pathogenic species.

抽象的： 人类微生物组项目提供了有关相关微生物的全面清单 与人体。下一个挑战是了解他们在人类健康和疾病中的作用 需要培养感兴趣的物种。与此应用有关，超过一半的口头 微生物被归类为未经培养的系统型，包括许多与口腔微生物有关的 发病。其中，候选部门TM7门的成员是最神秘的细菌 口腔中的小组。尽管在人体部位和其他环境中无处不在 TM7代表已被培养为纯文化。作为含有可疑病原体的门 与炎症性粘膜疾病有关，尤其是牙周炎，TM7的种植和能力 研究他们的致病特征已经变得像微生物学中的“圣杯”。 使用一种新型的培养基和富集技术，我们能够从A中降低含TM7的培养物 复杂的社区到包含疾病相关的TM7系统型（TM7X）的双物种共培养 与photopobium parvulum或Veillonella antypica一起作为伴侣菌株。我们假设 TM7X需要特定的合作伙伴物种，并且了解它们的关系可能是 隔离TM7X的纯文化。此外，TM7X的当前半纯化培养物和可能 通过这项研究获得的纯TM7X培养物将使我们能够研究基因组学的能力， 相互作用组和TM7X的致病潜力。该应用程序旨在检验我们的假设和 通过详细的TM7X与其合作伙伴之间关系的分子分析来实现这些目标 物种，改善培养纯TM7X的培养基，并探索其致病潜力。 该研究的直接影响是发现TM7X和相关的耕种条件 生物/生理功能。该研究的长期意义是创建新的途径 培养许多其他尚未培养的潜在致病物种。