SPECIALIZED ANIMAL RESOURCE MODULE

专业动物资源模块

• 批准号: 8266459
• 负责人: Timothy S Kern
• 金额: $ 10.13万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2012-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8266459
• 关键词: Age; Air; Air Conditioning; Anesthesia procedures; Animal Barrier Facility; Animal Model; Animal Technicians; Animals; Breeding; Budgets; Cancer Center Support Grant; Caring; Charge; Collaborations; Communication; Cornea; Defect; Doctor of Veterinary Medicine; Educational process of instructing; Environment; Eugenia; Eye; Funding; Genetically Engineered Mouse; Genotype; Grant; Growth; Health Status; Heating; Hour; Human Resources; Humidity; Individual; Infection; Injection of therapeutic agent; Knock-in Mouse; Knock-out; Maintenance; Medical center; Monitor; Mouse Strains; Mus; Mutant Strains Mice; Nature; Phenotype; Pilot Projects; Procedures; Production; Rattus; Records; Research; Research Personnel; Running; Schedule; Services; Surgeon; Tail; Temperature; Time; Tissue Sample; Toes; Training; Transgenic Animals; Transgenic Mice; Universities; Veterinarians; Virus; Vision; Vision research; Visit; Weaning; animal breeding; animal care; animal colony; animal resource; arm; base; cost; knockout gene; medical schools; meetings; mutant; offspring; programs; pup; ranpirnase; remote sensor

The Specialized Animal Resource (SAR) Module has been heavily used since the first funding cycle in 1997. Due to increased demand from current investigators and new investigators, we recently added a second full-time technician, Kathryn Zongolowicz, supported by Case Medical Center (CMC), to assist Heather Butler in breeding the large number of mouse strains utilized by VSRC investigators. We also added a part time (20%) veterinary surgeon, Eugenia Diaconu, also supported by CMC, to teach and assist investigators with difficult ocular injection procedures (e.g., corneal, intravitreal and subretinal). Mutant strains of mice and rats are of considerable value in modern research on the eye. Yet, within the current limitations on budgets, the costs of managing mutant strains is often too high to be added to individual investigator grants, especially for pilot projects. Proper handling of mutant strains represents a major problem in that special expertise is needed to establish the strain from valuable founders, overcome possible breeding problems caused by the mutant phenotype, manage the colonies efficiently in order to produce enough but not too many progeny of the right ages at the right time, and to obtain tissue samples (usually toe or tail snips under anesthesia) for determination of genotypes. With valuable strains, it is also necessary to use isolation cages to avoid infections that reduce the number of surviving progeny and can endanger the entire colony. In collaboration with the genotyping arm of the MB Module, the SAR Module provides a service of breeding, management, characterization and weaning of transgenic animals. In addition to animal management, Eugenia Diaconu, D.V.M. assists in the Module on a part-time basis. The SAR Module provides the critical support that allows our group of vision researchers to take greater advantage of genetically engineered mice and mutant rat lines. The Module was established, in a dedicated space in the Animal Resource Center (ARC) in the basement of the School of Medicine. However, due to the current extensive renovation of the animal care facility at the Case School of Medicine that should be completed by late 2007, the SAR Module breeding room is in the nearby Wolstein Research Building. This facility is a new state-of-the-art barrier animal facility dedicated only to virus-free mice. The facility is controlled for air flow, temperature and humidity, and these parameters are monitored continuously by remote sensors. The breeding facility is located within 200 yards of all investigators in the VSRC, and animals are transferred by the Animal Care Unit staff from the VSRC breeding center (and other breeding centers now located at Wolstein) to each investigator's colony daily or as needed. If more than a cage or two need to be transferred, animal transfer is accomplished in heated/air-conditioned vehicles specifically for this purpose. The ARC is fully accredited and employs a highly skilled staff, including three full-time veterinarians. Space within the facility is assigned on a need basis, and only our full-time veterinary technicians, participating investigators, and ARC staff have access to this room (Appendix A). The investigators of the VSRC have made a dramatic transition over the past five years to studying a large variety of mouse transgenic, knockout and knock-in lines. Having a module with extensive expertise in breeding challenging mouse strains and analyzing and documenting ocular defects meets a critical need for our investigators.

自 1997 年第一个资助周期以来，专业动物资源 (SAR) 模块已被大量使用。 由于现有调查员和新调查员的需求增加，我们最近增加了第二名全职技术人员， Kathryn Zongolowicz 在凯斯医疗中心 (CMC) 的支持下，协助 Heather Butler 繁育大量 VSRC 研究人员使用的小鼠品系。我们还增加了一名兼职 (20%) 兽医 Eugenia Diaconu， 还得到 CMC 的支持，教导和协助研究人员进行困难的眼部注射程序（例如角膜、 玻璃体内和视网膜下）。 小鼠和大鼠的突变品系在现代眼睛研究中具有相当大的价值。然而，在当前的 预算限制，管理突变菌株的成本往往太高，无法添加到个人研究者资助中， 特别是对于试点项目。突变株的正确处理是一个主要问题，因为需要特殊的专业知识 需要从有价值的创始人那里建立菌株，克服突变体可能引起的育种问题 表型，有效地管理菌落，以便在适当的年龄产生足够但不过多的后代 正确的时间，并获取组织样本（通常是麻醉下的脚趾或尾巴剪断）用于确定基因型。和 对于有价值的菌株，还需要使用隔离笼，以避免感染，从而减少存活后代的数量 并可能危及整个殖民地。与 MB 模块的基因分型部门合作，SAR 模块 提供转基因动物的育种、管理、表征和断奶服务。除了动物 管理，Eugenia Diaconu，D.V.M.兼职协助该模块。 SAR 模块提供了关键支持，使我们的视觉研究人员团队能够更好地利用 基因工程小鼠和突变大鼠系。该模块是在动物中心的专用空间内建立的 资源中心（ARC）位于医学院地下室。但由于目前正在进行大规模的改造 凯斯医学院的动物护理设施将于 2007 年底完工，SAR 育种模块 房间位于附近的沃尔斯坦研究大楼。该设施是一个新的、最先进的屏障动物设施，专门用于 只针对无病毒的小鼠。该设施对气流、温度和湿度进行控制，这些参数是 通过远程传感器连续监测。繁殖设施位于所有调查员 200 码范围内 VSRC，动物由动物护理部门工作人员从 VSRC 繁育中心（以及其他繁育中心）转移 中心（现位于沃尔斯坦）每天或根据需要前往每个研究者的群体。如果需要一两个以上的笼子 转移时，动物转移是在专门用于此目的的加热/空调车辆中完成的。弧线是 获得充分认可并雇用了高技能的员工，其中包括三名全职兽医。设施内的空间是 根据需要分配，只有我们的全职兽医技术人员、参与调查人员和 ARC 工作人员 进入该房间（附录 A）。 VSRC 的研究人员在过去五年中取得了巨大的转变，开始研究各种各样的 小鼠转基因、敲除和敲入系。拥有一个在培育具有挑战性的小鼠方面拥有丰富专业知识的模块 应变以及分析和记录眼部缺陷满足了我们研究人员的关键需求。