Integration of High Field MRI and EPRI For Functional Imaging

高场 MRI 和 EPRI 的集成用于功能成像

• 批准号: 8698417
• 负责人: JAY Louis ZWEIER
• 金额: $ 57.77万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8698417
• 关键词: Address; Algorithmic Software; Anatomic structures; Anatomy; Animal Disease Models; Animal Model; Animals; Back; Biochemical; Cardiac; Cardiovascular Physiology; Cardiovascular system; Cell Survival; Cell membrane; Cell physiology; Chemicals; Computer software; Consumption; Coupling; Data; Data Collection; Development; Disease; Electrons; Free Radicals; Frequencies; Functional Imaging; Generations; Heart; Heart Diseases; Image; Imaging technology; Injury; Life; Magnetic Resonance; Magnetic Resonance Imaging; Maps; Measurement; Measures; Mediator of activation protein; Metabolism; Modality; Modeling; Motion; Myocardial; Myocardial Infarction; Nitric Oxide; Noise; Organ; Organ Model; Oxidation-Reduction; Oxygen; Oxygen saturation measurement; Pathology; Perfusion; Physiological; Physiology; Power Sources; Protons; Reactive Oxygen Species; Respiration; Role; Sampling; Scanning; Source; Spin Trapping; Stress; System; Techniques; Technology; Testing; Time; Tissues; Work; base; cardiovascular injury; cell injury; free radical oxygen; heart function; image processing; image registration; imaging modality; in vivo; innovation; instrument; instrumentation; prevent; programs; prototype; public health relevance; software development; tool

DESCRIPTION (provided by applicant): Paramagnetic molecules including oxygen, oxygen radicals and nitric oxide modulate cellular function and injury, altering critical cellular processs such as respiration and redox state with changes in tissue perfusion, energetic state and viability. In view of the critical importance of these interactions in normal cardiovascular functin and disease, there has been a great need for an approach to combine in vivo measurement and imaging of paramagnetic molecules with imaging of tissue and organ biochemical and functional parameters in animal models of disease. EPR imaging (EPRI) is a powerful technique that enables 3D spatial in vivo mapping of radical metabolism, oxygenation, and nitric oxide; however, the utility and power of EPRI has been greatly limited by the need for complementary anatomic and functional data. NMR based MRI, in addition to providing anatomic registration, also can provide critical functional information that is needed to interpret the EPRI data including, imaging of tissue perfusion and viability. Integrated EPR/NMR imaging technology and instrumentation has the unique potential to enable in vivo mapping of free radicals, oxygen and nitric oxide along with NMR based functional and anatomic imaging. This technology is very promising but several fundamental problems presently limit its development and biomedical application. These include the need to: [a] provide seamless integration of the EPR and NMR-MRI systems to enable rapid transition between these two imaging modalities; [b] build dual mode resonators optimized for both EPR and NMR resonances suited for specific biomedical applications; [c] accelerate the process of image data collection in both modes facilitating dual mode anatomic and functional imaging; [d] increase sensitivity and minimize motion-induced noise or image distortion; [e] integrate the EPRI and MRI results and analysis in order to perform accurate image co-registration, fast computation and interpretation of functional data. These critical needs will be addressed with a combination of hardware and software development followed by testing in cardiovascular applications as described in 3 specific aims: I. Development and construction of EPRI hardware optimized for high sensitivity fast scan image acquisition and integration of it with high field MRI; II. Development / implementation of integrated software and algorithms for fast EPRI and NMR MRI acquisition with cardiac gating enabling co-mapping of anatomic structure, perfusion, energetic state and viability (MRI) with free radical distribution, redox state and oximetry (EPRI). EPRI acquisition will be accelerated and refined using NMR image information to refine EPR data collection; III. Application for dual mode functional imaging of the heart with both in vivo and ex vivo measurement of tissue O2 levels, redox status, nitric oxide formation (EPRI) in relation to perfusion, viability, and energeic state (MRI). This program will realize the great synergy and power of integrated real time EPRI and high field MRI optimized for biomedical measurement and imaging of free radicals, redox state, O2, and nitric oxide and their role in function and disease.

描述（由申请人提供）：顺磁分子，包括氧，氧自由基和一氧化氮调节细胞功能和损伤，改变了关键的细胞过程，例如呼吸和氧化还原状态，随着组织灌注，能量状态和生存能力的变化。鉴于这些相互作用在正常心血管功能和疾病中的重要性至关重要，因此非常需要一种方法来将流磁分子的体内测量和成像与组织和器官生化和功能参数的成像中的成像结合在一起。 EPR成像（EPRI）是一种强大的技术，可以实现3D空间在体内代谢，氧合和一氧化氮的体内映射。但是，EPRI的实用性和功能受到补充解剖和功能数据的需求受到极大限制。基于NMR的MRI，除了提供解剖学注册外，还可以提供关键的功能信息，以解释EPRI数据，包括组织灌注和生存能力的成像。集成的EPR/NMR成像技术和仪器具有独特的潜力，可以在自由基，氧气和一氧化氮以及基于NMR的功能和解剖成像的体内映射。这项技术非常有前途，但目前有几个基本问​​题限制了其发展和生物医学应用。其中包括：[a]提供EPR和NMR-MRI系统的无缝集成，以实现这两种成像方式之间的快速过渡； [b]构建针对适用于特定生物医学应用的EPR和NMR共振进行了优化的双模式谐振器； [c]在两种模式下加速图像数据收集的过程，促进双模式解剖和功能成像； [d]提高灵敏度并最大程度地减少运动引起的噪声或图像失真； [e]整合EPRI和MRI的结果和分析，以执行精确的图像共同注册，快速计算和功能数据的解释。这些关键需求将通过硬件和软件开发的结合来解决，然后在心血管应用中进行测试，如3个特定目的所述：I。优化的EPRI硬件的开发和构建，以高灵敏度快速扫描图像获取以及将其与高场MRI集成； ii。通过心脏控件的快速EPRI和NMR MRI获取的综合软件和算法的开发 /实施，可通过自由基分布，氧化还原状态和氧仪（EPRI）（EPRI）共同映射解剖结构，灌注，能量状态和生存能力（MRI）。 EPRI采集将通过NMR图像信息加速和完善，以完善EPR数据收集； iii。与灌注，活力和能量状态（MRI）有关，将心脏的双模式功能成像应用于组织O2水平，氧化还原状态，一氧化氮形成（EPRI）的双重功能成像。该程序将实现实时EPRI集成和高场MRI的巨大协同作用和力量，用于生物医学测量和自由基，氧化还原状态，O2和一氧化物的成像及其在功能和疾病中的作用。