Proton Electron Double Resonance Imaging (PEDRI)

质子电子双共振成像 (PEDRI)

• 批准号: 7030360
• 负责人: JAY Louis ZWEIER
• 金额: $ 73.14万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7030360
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; computer data analysis; computer human interaction; computer system design /evaluation; computer system hardware; electron spin resonance spectroscopy; free radicals; genetically modified animals; laboratory mouse; laboratory rat; molecular /cellular imaging; nuclear magnetic resonance spectroscopy

DESCRIPTION (provided by applicant): This proposal is for development of novel instrumentation to enable rapid measurement and imaging of free radicals in biomedical applications using Dynamic Nuclear Polarization (DNP) and Proton Electron Double Resonance Imaging (PEDRI). A unique variable field, rapid field cycling (FC) PEDRI instrument will be built capable of performing measurements from fields of 0 to 0.2 T. DNP/PEDRI can provide rapid high quality measurements of free radicals in living animals and these novel dual resonance techniques overcome a number of the fundamental limitations of EPR imaging that requires relatively long times for image data collection, provides limited image resolution due to the inherently broad linewidths of paramagnetic labels, and needs very large gradients as well as independent techniques for anatomic registration. Therefore, DNP and PEDRI have great potential to advance this field providing: sensitive, rapid and high quality measurements and imaging of paramagnetic molecules in biomedical systems. There are 5 aims: 1) Development of a magnet, gradient, field control, and system control interface optimized for PEDRI of free radicals in small animal models of disease; 2) Development of DNP spectroscopy and PEDRI with variable field EPR preexcitation for NMR based detection and imaging of the EPR spectra of free radicals; 3) Development of Field Cycled DNP and Spectral-Spatial PEDRI to enhance measurement sensitivity and minimize problems with power deposition and sample heating; 4) Development of innovative resonator and RF design optimized for DNP and PEDRI of in vivo and ex vivo biomedical applications; 5) Development of optimized software and algorithms enabling rapid acquisition and analysis of DNP and PEDRI spectral and image data. Together these aims will overcome existing limitations of RF power deposition and result in the development of a unique variable field rapid field cycling PEDRI/DNP instrument capable of rapid, high quality measurement and imaging of free radicals in small animal models of disease. This new instrumentation and related innovations in rapid field cycling, resonator technology and ultra fast image acquisition, have the potential to provide 100-fold enhanced sensitivity in radical detection, with 10-fold higher image resolution and over 10-fold faster image acquisition than prior fixed field PEDRI instrumentation, while reducing RF power deposition by over two orders of magnitude.

描述（由申请人提供）：该建议是用于开发新型仪器，以便使用动态核极化（DNP）和质子电子双共振成像（PEDRI）快速测量和成像生物医学应用中的自由基成像。将建立一个独特的可变场，快速场循环（FC）PEDRI仪器，能够从0到0.2 T. dnp/pedri的领域进行测量，可以提供对活动物中的自由基的快速高质量测量，这些新颖的双重共振技术克服了限制的EPR限制，以限制图像数据的基本限制。顺磁性标签，需要非常大的梯度以及独立的技术来进行解剖学登记。因此，DNP和PEDRI具有推进该领域的巨大潜力：敏感，快速和高质量的测量以及生物医学系统中顺磁分子的成像。有5个目的：1）开发磁铁，梯度，野外控制和系统控制界面，可针对小动物的疾病小动物模型优化自由基的PEDRI； 2）开发DNP光谱和PEDRI，具有可变的场EPR预言，用于基于NMR的自由基EPR光谱的基于NMR的检测和成像； 3）开发循环的DNP和光谱空间踏板，以增强测量灵敏度并最大程度地减少功率沉积和样品加热问题； 4）开发针对体内和体内生物医学应用的DNP和PEDRI优化的创新谐振器和RF设计； 5）开发优化的软件和算法，可以快速获取DNP和PEDRI光谱以及图像数据。这些目标将共同克服RF功率沉积的现有局限性，并导致能够快速，高质量测量和在小型疾病动物模型中对自由基进行快速，高质量测量和自由基的成像的独特田间快速场循环仪器的发展。这项新的仪器和相关的创新在快速场循环，谐振器技术和超快速图像获取中，有可能在自由基检测中提供100倍提高的灵敏度，并且比先前的固定现场PEDRI仪器更高的图像分辨率和超过10倍更快的图像获取，同时将RF的功率降低了两个级数的大量级数。