CELL BIOLOGY/MICROINJECTION

细胞生物学/显微注射

• 批准号: 8288285
• 负责人: Stephen A Murray
• 金额: $ 22.32万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8288285
• 关键词: Area; Cancer Center; Cancer Center Support Grant; Cellular biology; Collaborations; Competence; Cryopreservation; Custom; Derivation procedure; ES Cell Line; Electroporation; Ensure; Faculty; Fee-for-Service Plans; Funding; Gene Targeting; Grant; Health Services Accessibility; Human Resources; Hybrids; Inbred Strain; Inbred Strains Mice; Laboratories; Laboratory Research; Microinjections; Mission; Molecular Biology; Mus; Names; Phenotype; Process; Productivity; Reproductive Technology; Research; Research Personnel; Resources; Science; Scientist; Services; Technical Expertise; Technology; Training; Transgenic Organisms; Validation; Work; cost effective; embryonic stem cell; gene function; genetic resource; in vivo; instrumentation; meetings; member; operation; reagent testing; reproductive

The mission of the Cell Biology & Microinjection component of Reproductive Sciences is to provide Cancer Center members with access to centralized facilities, instrumentation and technical expertise necessary to allow investigators to carry out embryonic stem (ES) cell line derivation, electroporation, and to create genetically modified mice for in vivo studies of gene function. The Service generates genetically modified mice on a variety of inbred strain and hybrid backgrounds and has derived germline-competent ES cell lines from inbred mouse strains. Germline-competence validation, training, custom projects, and tested reagents and supplies are also offered. The Service, which has previously been supported by Cancer Center support grant (CCSG) funds, works closely with Cryopreservation, Importation and Rederivation within Reproductive Sciences, as well as Phenotyping Sciences to provide Cancer Center members well integrated comprehensive services. In 2004 the Cell Biology & Microinjection Service merged with the Reproductive Technologies Resource to form Reproductive Sciences. The synergy created by the merger allowed management and staff to identify complementary and overlapping functions within the groups; establish new collaborative service offerings; merge similar processes to eliminate redundancy; and combine laboratory, vivarium and office space to maximize productivity. Cell Biology & Microinjection Service personnel work closely with other members of Reproductive Sciences and the Molecular Biology Service to offer Cancer Center investigators seamless access to services. This close internal collaboration insures that investigators with expertise outside of these areas can fully employ current transgenic and gene targeting technology in their own work by utilizing these interactive services. Senior Staff Scientist Dr. Thomas Gridley was named Faculty Advisor (Project Leader) of Cell Biology & Microinjection Service in 1999. He oversees this fee-for-service operation which employs two ES cell technologists, two microinjection technologists, a colony manager and senior operational manager. The Service occupies 1,899 ft2 of laboratory, vivarium and office space in Research Laboratory Building 4 and the Genetics Resources Building. Dr. Gridley communicates with the Cancer Center users, Service staff and Center Administration to ensure that research needs are met in the most efficient, cost-effective and technically current manner.

生殖科学细胞生物学和显微注射部分的使命是提供癌症 中心成员可以使用必要的集中设施、仪器和技术专业知识 允许研究人员进行胚胎干 (ES) 细胞系衍生、电穿孔并创建 用于基因功能体内研究的转基因小鼠。该服务生成转基因 具有多种近交系和杂交背景的小鼠，并衍生出具有种系能力的 ES 细胞 来自近交系小鼠品系。种系能力验证、培训、定制项目和测试 还提供试剂和用品。该服务此前曾得到癌症中心的支持 中心支持拨款 (CCSG) 资金，与冷冻保存、进口和再衍生密切合作 在生殖科学以及表型科学领域为癌症中心成员提供良好的服务 一体化综合服务。 2004 年，细胞生物学和显微注射服务与 生殖技术资源形成生殖科学。合并产生的协同效应 允许管理层和员工确定小组内互补和重叠的职能； 建立新的协作服务产品；合并相似的流程以消除冗余；和 将实验室、动物园和办公空间结合起来，以最大限度地提高生产力。细胞生物学与显微注射 服务人员与生殖科学和分子生物学的其他成员密切合作 为癌症中心研究人员提供无缝服务访问的服务。这种密切的内部合作 确保具有这些领域之外专业知识的研究人员能够充分利用当前的转基因和 基因打靶技术通过利用这些交互式服务在自己的工作中发挥作用。高级研究员科学家博士 Thomas Gridley 被任命为细胞生物学和显微注射服务学院顾问（项目负责人） 1999 年。他负责监督这项按服务收费的业务，该业务雇用了两名 ES 细胞技术人员、两名 显微注射技术人员、群体经理和高级运营经理。该服务占用 研究实验室 4 号楼和遗传学实验室、动物园和办公空间占地 1,899 平方英尺 资源建设。格里德利博士与癌症中心用户、服务人员和中心进行交流 确保以最高效、最具成本效益和技术性的方式满足研究需求的管理 目前的方式。