Resource Section

资源部分

• 批准号: 10223459
• 负责人: Stephen A Murray
• 金额: $ 13.22万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-18 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10223459
• 关键词: Animal Model; Archives; Biology; Breeding; CRISPR/Cas technology; Collection; Communities; Development; Disease model; Engineering; Evaluation; Funding; Future; Genes; Genetic; Genotype; Goals; Infrastructure; Knockout Mice; Leadership; Light; Maintenance; Mission; Modeling; Mus; Mutant Strains Mice; Phenotype; Preclinical Testing; Procedures; Process; Production; Recording of previous events; Reporter; Resources; Role; Science; Ships; Technology; Testing; The Jackson Laboratory; United States National Institutes of Health; Validation; animal model development; cohort; experience; flexibility; gene therapy; genetic resource; genome editing; innovation; model development; mouse model; novel; programs; quality assurance; repository; somatic cell gene editing; success; technology development; tool; transmission process

PROJECT SUMMARY RESOURCE CORE The overall goal of the Somatic Cell Genome Editing (SCGE) consortium is to accelerate the development of translational applications of genome-editing technology, particularly CRISPR/Cas9, taking advantage of recent successes in gene therapy treatments. The specific goal of the Jackson Laboratory Genome Editing Mouse Testing Center (JAX-GEMTC) is to develop novel mouse resources to facilitate the validation and optimization of new genome editing technologies. The RESOURCE CORE of the JAX-GEMTC will support mouse breeding and colony expansion for genotypic and functional validation of reporters, cohort production, and integration with the NIH-funded Mouse Mutant Regional Resource Centers (MMRRC’s). The resource will take advantage of the high-throughput colony management infrastructure of the JAX KOMP2 program and the mouse resource distribution experience of the JAX Repository. Public availability of these new resources will be facilitated by the co-localization of the Center with the JAX MMRRC. The Resource goals of this Center will be achieved through two Specific Aims: 1) Establish production-scale breeding colonies for production of reporter line cohorts for the JAX-GEMTC. The goal of this Aim is to build production colonies of reporter mice to support all aspects of the JAX-GEMTC program. Initially, we will import the existing Traffic Light Reporter (TLR) model to JAX and then the importation additional reporters will follow. Models engineered at JAX as part of the Animal Model Production section will be screened for germline transmission, and production colonies of all models will be established for functional validation, baseline testing, and to provide cohorts for the TESTING CORE. 2) Archive and distribute reporter lines to the scientific community through the JAX-MMRRC. In this aim we will provide novel reporter lines and all associated information for their use to the MMRRC for distribution to the scientific community. To accomplish this we will take advantage of JAX’s role as one of the MMRRC centers, facilitating the submission, archiving, and distribution process.

项目摘要资源核心 体细胞基因组编辑（SCGE）联盟的总体目标是加速体细胞基因组编辑的发展 基因组编辑技术，特别是 CRISPR/Cas9 的转化应用，利用最近的成果 杰克逊实验室基因组编辑小鼠的具体目标。 测试中心（JAX-GEMTC）将开发新颖的鼠标资源，以方便验证和优化 JAX-GEMTC 的资源核心将支持小鼠育种。 和菌落扩展，用于生产者的基因型和功能验证、队列生产和整合 与 NIH 资助的小鼠突变体区域资源中心 (MMRRC) 合作，该资源将得到充分利用。 JAX KOMP2 程序的高通量菌落管理基础设施和小鼠资源 JAX 存储库的分发经验将通过以下方式促进这些新资源的公开可用性。 该中心与 JAX MMRRC 的共同定位 该中心的资源目标将得以实现。 通过两个具体目标： 1) 建立用于生产报告系的生产规模育种群体 JAX-GEMTC 的队列 该目标的目标是建立报告小鼠的生产群体以支持所有小鼠。 JAX-GEMTC 计划的各个方面 最初，我们将导入现有的交通灯报告器 (TLR) 模型。 JAX 以及随后导入的其他生产商将作为动物的一部分在 JAX 设计。 模型生产部分将进行种系传播筛查，所有模型的生产菌落将 建立功能验证、基线测试，并为测试核心 2) 提供队列。 为实现这一目标，我们将通过 JAX-MMRRC 存档并向科学界分发报告线路。 向 MMRRC 提供新颖的报告线路和所有相关信息，以便分发给 为了实现这一目标，我们将利用 JAX 作为 MMRRC 中心之一的作用， 促进提交、归档和分发过程。