Cell Biology and Histology

细胞生物学和组织学

• 批准号: 8734480
• 负责人: JOAN HELLER BROWN
• 金额: $ 26.02万
• 依托单位: SAN DIEGO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8734480
• 关键词: Apoptotic; Area; Biological Assay; Blood Vessels; California; Cardiac; Cardiac Myocytes; Cell Separation; Cell physiology; Cells; Cellular Structures; Cellular biology; Coronary; Differentiation Antigens; Ensure; Equipment; Fibrosis; Genetically Engineered Mouse; Healed; Heart; Histology; Histopathology; Housing; Human; Image; Immunofluorescence Immunologic; In Situ Nick-End Labeling; Instruction; Maintenance; Microscopic; Mus; Muscle Cells; Myocardial; Myocardial Infarction; Oxygen; Preparation; Proto-Oncogene Protein c-kit; Resources; Sampling; Services; Signal Transduction; Signaling Molecule; Sorting - Cell Movement; Staining method; Stains; Stem cells; Structure; Tissues; Training; Universities; caspase-3; healing; in vivo; response

The Cell Biology and Histology Core (CBHC) is a critical resource to this PPG and will be used by all Projects. The CBHC will provide the projects with the following services: 1. preparation of cardiac progenitor cells (CPCs): isolation, characterization, and storage of CPCs from wild type and genetically modified mice, 2. preparation of stable lentiviral expressing lines of CPCs, 3. immunohistopathology: Preparation of histological sections for all projects, histopathology to assess gross and microscopic myocardial structure, fluorescent microscopic imaging of CPCs in heart section, analysis of vasculature. The CBHC will be located at the University of California, San Diego. The CBHC will house and maintain the equipment and provide fully trained staff for the Projects. It will provide the four projects with access to a wide variety of assays and analyses, including CPC isolation, sorting and characterization, assays of CPC function, and histopathology of heart samples obtained in vivo. CBHC services will also include isolation, maintenance characterization of CPCs from genetically engineered mice for the projects. CBHC will also expand and characterize the human CPCs isolated by Dr. Sussman for use by other projects. CBHC will ensure that all four projects will use CPCs that have been isolated, expanded and sorted the same way. This consistency is extremely important for the integration and comparison of results between the projects. The CBHC will also assist all the Projects with preparation of histological sections and histopathology to assess gross and microscopic myocardial structure, including the extent of fibrosis, quantification of chamber size and myocyte cross sectional area. It will also analyze heart sections for presence of apoptotic cells by TUNEL and anti-deaved caspase-3 immunofluorescence staining, and provide microscopic assessment of cardiac cell structure and signaling molecule localization via immunofluorescence of mouse heart sections. Finally, the CBHC will stain heart sections to detect c-kit positive CPCs plus markers of differentiation and proliferation, and prepare coronary vascular casts. Uniform preparation and analysis of histological sections from hearts are also important for consistent and comparable results between the four projects. RELEVANCE (See instructions); Heart muscle cells die when the tissue lacks oxygen, as occurs with a myocardial infarction (Ml; heart attack). Here we isolate progenitor cells that we believe could replace these dying cells and determine how to make them better able to heal the heart. We also examine the response of these cells within the heart and their effects on cardiac damage following Ml.

细胞生物学和组织学核心（CBHC）是该PPG的关键资源，将由所有人使用 项目。 CBHC将为项目提供以下服务： 1。制备心脏祖细胞（CPC）：从CPC中的隔离，表征和存储 野生型和转基因小鼠， 2。稳定慢病毒表达CPC的线的制备， 3。免疫组织病理学：为所有项目的组织学部分准备，以评估组织病理学 总体和微观心肌结构，心脏部分中CPC的荧光微观成像， 脉管系统分析。 CBHC将位于加利福尼亚大学圣地亚哥分校。 CBHC将容纳并维护 设备并为项目提供全面训练的员工。它将为这四个项目提供访问 各种各样的测定和分析，包括CPC隔离，排序和表征，CPC测定法 在体内获得的心脏样本的功能和组织病理学。 CBHC服务还将包括隔离， 来自基因工程小鼠的CPC的维护表征。 CBHC也将 扩展和表征Sussman博士隔离的人类CPC，以供其他项目使用。 CBHC会 确保所有四个项目都将使用已隔离，扩展和分类的CPC使用相同的方式。这 一致性对于整合和比较结果非常重要 项目。 CBHC还将为所有项目提供组织学部分的准备和 组织病理学评估总和微观心肌结构，包括纤维化的程度， 腔室大小和心肌横截面区域的定量。它还将分析心脏部分 通过TUNEL和抗deateved caspase-3免疫荧光染色和抗朝向的caspase-3的凋亡细胞的存在，并且 通过微观评估心脏细胞结构和信号分子的定位 小鼠心脏切片的免疫荧光。最后，CBHC将染色心脏截面以检测C-KIT 阳性CPC以及分化和增殖的标志物，并制备冠状动脉血管铸件。制服 对心脏组织学部分的准备和分析对于一致和 四个项目之间的可比结果。 相关性（请参阅说明）； 当组织缺氧时，心肌细胞会死亡，就像心肌梗塞（ML；心脏）发生的那样。 攻击）。在这里，我们分离出我们认为可以取代这些垂死的细胞的祖细胞，并确定如何 使他们能够更好地治愈心脏。我们还检查了这些细胞在心脏和 它们对ML后心脏损伤的影响。