Mechanisms of epidermal growth during development, homeostasis, and tumorigenesis

发育、稳态和肿瘤发生过程中表皮生长的机制

基本信息

批准号：
8726283
负责人：
Slobodan Beronja
金额：
$ 22.19万
依托单位：
FRED HUTCHINSON CANCER RESEARCH CENTER
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-01 至 2016-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8726283
关键词：
Academia Address Adult Behavior Bioinformatics Biological Biological Process Biology Biometry Cancer Biology Candidate Disease Gene Cells Clinical Research Complex Computational Biology Development Developmental Biology Disease Educational workshop Embryo Ensure Environment Epithelial Equipment Foundations Funding Agency Genes Goals Grant Growth Health Homeostasis Human Human Development Hyperplasia In Vitro Instruction Investigation Laboratories Malignant Neoplasms Mediating Memorial Sloan-Kettering Cancer Center Methods Modeling Molecular Mus Mutation National Institute of Arthritis and Musculoskeletal and Skin Diseases Nature Neonatal Oncogenes Oncogenic Organ Organism Pathway interactions Physiological Positioning Attribute Process RNA Interference Regulation Research Research Personnel Resources Role Scientist Skin Solid Staging System Tissues Training United States National Institutes of Health Universities Work anticancer research base career career development design experience follow-up gene function genome wide association study genome-wide in vivo insight interest lectures medical schools meetings novel originality post-doctoral training programs repaired research and development screening self-renewal skills therapeutic target tumor tumorigenesis

项目摘要

Project Summary Throughout the graduate and postdoctoral stages, my goal has been to get training and experience that will allow me to establish an independent and successful research program in developmental and cancer biology. Specifically, I am interest in the mechanisms that regulate epithelial tissue development and homeostasis, and to changes that occur in disease states such as cancer. My plan for the reminder of the postdoctoral training is to acquire additional skills and develop research material that I will be able to carry into an independent position in academia. The Rockefeller University is an ideal place for the development of my career as an independent researcher. The University is a part of the Tri-Institutional Program, together with Memorial Sloan-Kettering Cancer Center and Weill-Cornell Medical College, which is a premier environment to conduct research, interact with fellow scientists, and attend lectures by leaders in a variety of fields. The Tri-Institutional group also organizes workshops in bioinformatics and biostatistics, and lectures dedicated to clinical and cancer research, both of which are the focus of my training. Furthermore, here I will interact with ollaborators that are experts in computational and cancer biology. Lastly, the resources within the Fuchs laboratory and the Resource Centers present at the Rockefeller University will provide equipment, training, and technical help that will ensure successful completion of the proposed research. In order to meet my research and career development goals I propose to conduct a genome-wide, RNAi-mediated modifier screen in vivo, to uncover regulatory mechanisms that govern epidermal tissue growth and homeostasis during development and in oncogene-induced hyperplasia. I expect that this comprehensive work will result in identification of many candidate genes that specifically operate within the physiological environment. The high-throughput nature and the originality of the approach that I propose, will allow me to complete postdoctoral training, and in the process set up a solid foundation for the future research program. At the start of the independent stage, I will base my research on identifying mechanisms used by selected candidates, to regulate tissue growth in the models of embryonic skin development and epidermal tumorigenesis. I will use these findings in subsequent grant support applications to the NIH (NIAMS and NCI), and other available sources of funding.

项目摘要在整个研究生和博士后阶段，我的目标是获得培训和经验请允许我建立一个独立的发展和癌症生物学研究计划。具体而言，我对调节上皮组织发育和稳态的机制感兴趣，以及在癌症等疾病状态中发生的变化。我提醒博士后培训的计划是获得其他技能，并开发我能够在学术界独立职位的研究材料。洛克菲勒大学是我作为独立职业生涯发展的理想场所研究员。该大学是三机构计划的一部分，以及纪念斯隆 - 凯特林癌症中心和威尔 - 康奈尔医学院（Weill-Cornell Medical College），这是一个主要的环境，与其他领域的领导者进行研究，与其他科学家进行互动并参加讲座。三机构小组还组织了生物信息学和生物统计学的研讨会，并致力于致力于临床和癌症研究的讲座，这都是我培训的重点。此外，在这里，我将与计算和癌症生物学专家的Ollaborator进行互动。最后，Fuchs实验室和洛克菲勒大学的资源中心内的资源将提供设备，培训和技术帮助，以确保成功完成拟议的研究。为了实现我的研究和职业发展目标，我建议在体内进行整个基因组，RNAi介导的修饰符筛选，以发现控制表皮组织生长和在发育过程中以及癌基因诱导的增生过程中的表皮组织生长和稳态的调节机制。我希望这项全面的工作将导致鉴定许多在生理环境中专门运作的候选基因。我建议的方法的高通量性质和原创性将使我能够完成博士后培训，并在此过程中为未来的研究计划奠定了坚实的基础。在独立阶段开始时，我将基于确定所选机制的研究候选者，以调节胚胎皮肤发育和表皮肿瘤发生模型中的组织生长。我将在NIH（NIAMS和NCI）以及其他可用资金来源的后续赠款支持申请中使用这些发现。