Exposure core

曝光核心

• 批准号: 8535762
• 负责人: Carl W White
• 金额: $ 12.46万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8535762
• 关键词: Academic Medical Centers; Acids; Acute Lung Injury; Aerosols; Air; Alteplase; Anesthesia procedures; Anesthetics; Animal Experiments; Animal Housing; Animals; Anticoagulants; Antioxidants; Appearance; Area; Autopsy; Biochemical; Blood capillaries; Body Fluids; Breathing; Caliber; Certification; Chemical Models; Chemicals; Chlorine; Collaborations; Color; Colorado; Computers; Conscious; Cross-Over Studies; DNA Damage; Data; Distress; Edema; Ensure; Environmental air flow; Evaluation; Event; Exposure to; Free Radicals; Gases; Head; Health; Hour; Human Resources; Inflammation; Injury; Intervention Studies; Laboratories; Laboratory Procedures; Liquid substance; Lung; Maintenance; Mechlorethamine; Medical; Methods; Modeling; Monitor; Mustard; Mustard Gas; New Agents; Nose; Operative Surgical Procedures; Perfusion; Peripheral; Pharmaceutical Preparations; Plasminogen Activator; Poison; Preparation; Procedures; Process; Protocols documentation; Publications; Pulmonary function tests; Pulse Oximetry; Qualifying; Quality Control; Rattus; Records; Regulation; Reporting; Research Personnel; Resources; Rodent; Safety; Sampling; Site; Skin; Stethoscopes; Structure of parenchyma of lung; Supervision; System; Techniques; Testing; Therapeutic Intervention; Thrombolytic Therapy; Time; Tissue Procurements; Toxic effect; Travel; Universities; Vertebrates; Vesicants; Weight; Whole Body Plethysmography; Work of Breathing; Writing; animal care; base; capillary; chlorine gas; computerized data processing; data modeling; follower of religion Jewish; health record; in vivo; interest; lung injury; member; novel therapeutics; programs; protective effect; research study; silibinin; sound

The Exposure Core consists of an AinA/ay (Lung) Exposure Core based in Denver at National Jewish Health and a Skin Exposure Core based at University of Colorado Denver on the medical campus. The Ainway (Lung) Exposure Core consists of an offsite exposure facility at USAMRICD (Aberdeen, MD) subcontracted for exposure of rats to SM (described herein) supervised by our collaborator Dana Anderson, and Denver (National Jewish Health)-based exposure facilities for ain/vay exposures to half-mustard (2-chloroethyl ethylsulfide, CEES; nose-only system) and chlorine (whole body). The former provides an ethanolic aerosol (0.6 micron mass median diameter) by nose-only delivery to anesthetized rats in an established model. The latter provides chlorine gas in dry dilution air by whole body exposure to conscious rats in a more recently constructed system that is functioning and providing results similar to those reported by others with similar levels of exposure. Verification of exposure levels in the chlorine system is based on the 'sulfamic acid trap' method previously described Rando and colleagues. Processing of lung tissue and body fluids from rats exposed to these three toxic inhaled gases is illustrated in schematic diagram. For studies requiring airway instillation of drugs (eg tPA) with SM exposure, a small group of investigators (1) and technicians (2) from National Jewish will travel to USAMRICD 2-4 times annually to assist in these more labor-intensive studies. Pulmonary function testing (flexiVent) is available nearby to CEES and chlorine exposure systems in Denver, and also at the SM facility in Aberdeen (Buxco whole body plethysmography). The latter is described herein and the former is in Project 1. The Ainway Exposure Core is heavily used mainly by Projects 1 and 2. The Skin Exposure Core during the first cycle established an in vivo exposure system for CEES that can cause reproducible microvesication, DNA damage, inflammation and edema, and more recentiy has developed a nitrogen mustard exposure system that results in all of these events but with much more marked potency. The Core includes interactive studies will all 3 Projects.

暴露核心由丹佛的AINA/AY（肺）暴露核心组成，以及位于科罗拉多州丹佛分校的医学院丹佛分校的皮肤暴露核心。 Ainway（肺）暴露核心包括在Usamricd（马里兰州阿伯丁市）的异地暴露设施组成，分包了将大鼠暴露于SM（此处描述的）由我们的合作者Dana Anderson和Denver（National Jewish Health）（国家犹太人健康）基于AIN/VAY-MUSTOURAS的曝光设施，以实现AIN/VAY-MUSTORES的暴露设施 乙基硫化物，CEES；仅鼻子系统）和氯（全身）。前者通过在既定模型中向麻醉大鼠递送到麻醉大鼠的乙醇气溶胶（0.6微米质量中值直径）。后者通过全身暴露于最近构造的系统中的有意识大鼠在干稀释空气中提供氯气，该系统正常运行，并提供了与其他人报告的结果相似的结果 暴露水平。氯系统中暴露水平的验证基于先前描述的Rando及其同事的“磺酸陷阱”方法。示意图中说明了暴露于这三种有毒吸入气体的大鼠的肺组织和体液的处理。对于需要气道滴注药物（例如TPA）的研究，一小部分研究人员（1）和技术人员（2） 国家犹太人每年将2-4次前往USAMRICD，以协助这些更加劳动密集型研究。 在丹佛的CEES和氯暴露系统附近以及在Aberdeen的SM设施（Buxco全身ProplesyMmography）附近可用肺功能测试（Flexivent）。后者在本文中描述了后者，前者在项目1中。AINWay暴露核心主要由项目1和2进行大量使用。在第一个周期中，皮肤暴露核心建立了CEES的体内暴露系统，该系统可能会导致可重复性的微神经，DNA损伤，炎症，炎症和湿气，以及更多的氮气曝光系统，这些事件都在这些事件中得到了更多的影响。核心包括互动研究将所有3个项目。