High Speed 10-Color Flow Cytometer

高速 10 色流式细胞仪

• 批准号: 8247492
• 负责人: Debra L Laskin
• 金额: $ 22.24万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247492
• 关键词: 9 year old; Asthma; Biomedical Research; Cells; Color; Core Facility; Cytometry; Dentistry; Diabetes Mellitus; Disease; Dyes; Flow Cytometry; Funding; Green Fluorescent Proteins; Image Analysis; Joints; Malignant Neoplasms; Medicine; Monoclonal Antibodies; New Jersey; Population; Research; Research Personnel; Services; Speed; United States National Institutes of Health; Universities; Wood material; instrument; medical schools; novel

DESCRIPTION (provided by applicant): The application of high speed multiparameter flow cytometry has revolutionized many aspects of biomedical research. Paired with the use of monoclonal antibodies, green fluorescent proteins, and other fluorescent indicator dyes, cell subsets have been identified and characterized that have led to novel treatments for diseases such as cancer, asthma, and diabetes. A group of 33 NIH-supported researchers at Rutgers University and the University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School are requesting funds to purchase a Beckman Coulter Gallios flow cytometer to replace a 9 year old cytometer in the joint University's Shared Analytical Cytometry/Image Analysis Core Facility. The instrument chosen will provide the critical capability of analyzing rar cell populations at rapid rates with exceptionally high sensitivity and stability. The majority of he users have previously used services of the Core facility for their research. The requested instrument will expand their capabilities and increase the pace of NIH supported scientific research.

描述（由申请人提供）：高速多参数流式细胞术的应用彻底改变了生物医学研究的许多方面。与使用单克隆抗体，绿色荧光蛋白和其他荧光指示剂染料的使用相结合，已经确定并表征了细胞子集，这些细胞子集已导致了针对癌症，哮喘和糖尿病等疾病的新型治疗方法。罗格斯大学（Rutgers University）以及新泽西 - 罗伯特·伍德·约翰逊医学院（New Jersey-Robert Wood Johnson）医学院的33个由NIH支持的研究人员组成的研究人员，要求资金购买贝克曼·库尔特·加利奥斯（Beckman Coulter Gallios）流式细胞仪，以取代联合大学共享的分析分析仪/图像分析核心核心设施中的9年历史细胞仪。所选的仪器将提供以极高的灵敏度和稳定性快速分析RAR细胞种群的关键能力。大多数HE用户以前曾使用核心设施的服务进行研究。所请求的工具将扩大其能力，并提高NIH支持科学研究的步伐。

项目成果

Simultaneous determination of 3-mercaptopyruvate and cobinamide in plasma by liquid chromatography-tandem mass spectrometry.

液相色谱-串联质谱法同时测定血浆中3-巯基丙酮酸和可滨酰胺。

• DOI: 10.1016/j.jchromb.2015.11.027
• 发表时间: 2016
• 期刊: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
• 作者: Stutelberg,MichaelW;Dzisam,JosephK;Monteil,AlexandreR;Petrikovics,Ilona;Boss,GerryR;Patterson,StevenE;Rockwood,GaryA;Logue,BrianA
• 通讯作者: Logue,BrianA