Caliper Life Sciences IVIS Lumina II imaging system

Caliper 生命科学 IVIS Lumina II 成像系统

• 批准号: 8246848
• 负责人: BRAD T COOKSON
• 金额: $ 16.11万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-15 至 2013-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246848
• 关键词: Accounting; Animal Experimentation; Animals; Biological Sciences; Cells; Communicable Diseases; Communities; Coupled; Data; Data Set; Diagnosis; Dimensions; Euthanasia; Faculty; Fostering; Funding; Gene Expression; Gene Expression Regulation; Hypersensitivity; IACUC; Image; Immune response; Inbred Strain; Individual; Infectious Agent; Inflammatory Bowel Diseases; Inflammatory Response; Kinetics; Laboratories; Life; Location; Malignant Neoplasms; Medical; Medical center; Medicine; Microbiology; Monitor; Mus; Pathology; Postdoctoral Fellow; Productivity; Protocols documentation; Public Health; Reporter; Research; Research Personnel; Rodent Model; Schools; Specific Pathogen Frees; Stimulus; Study models; System; Technology; Time; Training Programs; Training Support; United States National Institutes of Health; Universities; Variant; Washington; advanced system; animal facility; germ free condition; global health; graduate student; human disease; in vivo; insight; instrument; interest; novel; pathogen; programs; research study; response

DESCRIPTION (provided by applicant): The University of Washington Medical Center seeks the acquisition of a Caliper Life Sciences IVIS(R) Lumina II Imaging System, which will provide new experimental technology for our specific pathogen free (SPF) ABSL-2 animal facility. This instrument utilizes an ultrasensitive camera to detect both bioluminescent and fluorescent reporters within living animals, allowing users to collect anatomically localized longitudinal data on cell-specific fates, gene expression and the kinetics and distribution of replicating infectious agents within individual living animals without the need for euthanasia. This system dramatically increases the amount of data that can be collected from individual animals and reduces the number of animals required for each experiment. This technology adds the dimension of time to data sets, which is a powerful addition to experiments when coupled with the ability of the instrument to account for individual mouse variation in response to stimuli, even among genetically identical inbred strains. The acquisition of an IVIS(R) Lumina II is essential for the University of Washington to maintain its high quality of research and level of productivity, and to facilitate the expansion of the research programs carried out by several NIH-funded laboratories within the Departments of Microbiology, Laboratory Medicine, Pathology, Global Health, and Allergy/Infectious Disease. These laboratories share a common interest in host-pathogen interactions. Using rodent models of human disease, researchers will use this imaging system for in vivo monitoring of infectious diseases, immune and inflammatory responses, and gene regulation. Each of these fields of research is highly relevant to public health, enhancing our understanding of malignancy, inflammatory bowel disease, and infectious diseases of significance for global health. The animal research group that will use this in vivo imaging system consists of six major users (Cookson, Crispe, Smith, Fang, Hawn, and Skerrett). The major users are faculty within a large number of graduate and medical training programs, which support the training of an ever-increasing depth and breadth of graduate students and post-doctoral fellows. We anticipate that the centralized location of this unique and powerful technology at the University Of Washington School Of Medicine will attract new users within the animal research community, which includes over 400 faculties with IACUC approved protocols. Acquisition of the Caliper Life Sciences IVIS(R) Lumina II Imaging System will foster novel insight into human disease through the study of model animal systems, and advance our understanding, diagnosis and treatment of human diseases. PUBLIC HEALTH RELEVANCE: The IVIS(r) Lumina II uses an ultrasensitive camera to detect light from reporter genes and will provide anatomically localized information about cellular activities, gene expression, inflammation and infection in living animals. Importantly, the temporal aspects of these activities are also revealed. Thus, important diagnostic and potentially therapeutic information about important problems such as cancer, infection and autoimmune diseases will be provided from the studies in this application.

描述（由申请人提供）：华盛顿大学医学中心寻求获得卡钳生命科学IVIS（R）Lumina II成像系统，该系统将为我们的特定病原体（SPF）ABSL-2动物设施提供新的实验技术。该仪器使用超敏感相机来检测活动物中的生物发光和荧光记者，从而使用户可以收集解剖上局部的纵向数据 在细胞特异性的命运上，基因表达和动力学和复制感染性的分布 单个活动物中的特工无需安乐死。该系统大大增加了可以从单个动物中收集的数据量，并减少了每个实验所需的动物数量。这项技术增加了数据集的时间尺寸，这是对实验的有力补充，与仪器的能力相结合，即使在遗传上相同的杂交菌株中，该仪器能够解释各个小鼠变化。对华盛顿大学保持其高质量的研究和生产率以及对 有助于扩大微生物学，实验室医学，病理学，全球健康和过敏/传染病的部门内的几家NIH资助的实验室进行的研究计划。这些实验室在宿主 - 病原体相互作用中具有共同的兴趣。使用人类疾病的啮齿动物模型，研究人员将使用此成像系统在体内监测传染病，免疫和炎症反应以及基因调节。这些研究领域中的每一个都与公共卫生高度相关，增强了我们对恶性肿瘤，炎症性肠病和对全球健康显着性的传染病的理解。将使用此体内成像系统的动物研究小组由六个主要用户（Cookson，Crispe，Smith，Fang，Hawn和Skerrett）组成。主要用户是大量研究生和医学培训计划中的教职员工，这些课程支持培训研究生和博士后研究员的深度和广度。我们预计，华盛顿大学医学院这一独特而强大的技术的集中位置将吸引动物研究界的新用户，其中包括400多名拥有IACUC批准协议的能力。获得卡钳生命科学IVI（R）Lumina II成像系统的获取将通过研究模型动物系统来促进对人类疾病的新见解，并提高我们对人类疾病的理解，诊断和治疗。 公共卫生相关性：IVIS（R）Lumina II使用超敏感相机来检测报告基因的光，并将提供有关活动物中细胞活性，基因表达，炎症和感染的解剖局部局部信息。重要的是，这些活动的时间方面也得到了揭示。因此，将从本应用中的研究中提供有关重要问题（例如癌症，感染和自身免疫性疾病）的重要诊断和潜在的治疗信息。