WU-MDACC Inter-Institutional Molecular Imaging Center

WU-MDACC 跨机构分子影像中心

• 批准号: 8536730
• 负责人: Samuel Achilefu
• 金额: $ 133.6万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8536730
• 关键词: Address; Basic Science; Biochemical Reaction; Biological Assay; Bone Marrow Transplantation; Cells; Chemistry; Clinical Research; Collaborations; Diagnostic; Discipline; Epigenetic Process; Event; Explosion; Foundations; Gene Expression; Image; Inflammation; Interdisciplinary Study; Lead; Libraries; Life; Lymphocyte; Malignant Neoplasms; Metabolic stress; Molecular; Monitor; Mus; Organism; Patients; Pilot Projects; Positron-Emission Tomography; Postdoctoral Fellow; Process; Regulatory Pathway; Reporter; Research Personnel; Research Project Grants; Resources; Robotics; Signal Transduction; Signaling Protein; Small Interfering RNA; Students; Techniques; Therapeutic; Training Programs; Transgenic Organisms; Translational Research; Tumorigenicity; Universities; Viral; Washington; Whole Organism; cancer therapy; chemical genetics; clinical application; fundamental research; genetically modified cells; high throughput screening; in vivo; interest; molecular imaging; multidisciplinary; notch protein; novel; prognostic; programs; trafficking

DESCRIPTION (provided by applicant): Ground-breaking molecular imaging studies have visualized gene expression, biochemical reactions, signal transduction and regulatory pathways in living cells and whole organisms in vivo. The Washington University Molecular Imaging Center directs major efforts into fundamental, translational and clinical research under the theme of imaging signal transduction and therapeutics in cancer using novel techniques and probes in combination with genetically-encoded reporters to pinpoint molecular events within target cells. The Washington University Molecular Imaging Center activities have spurred an explosion of collaborative, interdisciplinary research projects that have formed the foundation for exciting new directions in molecular imaging. A dedicated team of investigators have demonstrated an exceptional record of collaboration outside their own disciplines leading to an escalating interest in applying novel genetically- encoded reporter strategies that allow the molecular characterization of cancer in vivo for basic research as well as diagnostic and prognostic clinical applications. In particular, this has lead to a major theme to study regulated signal transduction and protein processing in living organisms. The principal research projects address imaging of Notch signaling in cancer, investigate metabolic stress and checkpoint controls as targets for anticancer therapy, investigate the interaction of inflammation and viral-induced tumorigenicity in doubly transgenic reporter mice, and monitor trafficking of genetically-modified cells with PET during epigenetic modulation of donor lymphocytes in a clinical study of patients undergoing bone marrow transplant. The program also includes three molecular imaging scientific resources: the molecular imaging reporter core, chemistry core and high throughput screening robotics core, founded on molecular imaging platforms for readout of a wide range of bio-assays, including siRNA library screens, and chemical genetics. Our Molecular Imaging Center also provides a pilot project program for new investigators and a multidisciplinary training program for students, post-docs, and fellows.

描述（由申请人提供）：开创性的分子成像研究已可视化基因表达，生化反应，信号转导和活体细胞和整个生物体的调节途径。华盛顿大学分子成像中心将主要的努力用于基本，转化和临床研究，以成像信号转导和癌症的疗法的主题使用新技术和探针结合遗传编码的记者，以在靶细胞中查明分子事件。华盛顿大学分子成像中心的活动刺激了协作，跨学科研究项目的爆炸，这为令人兴奋的分子成像方向奠定了基础。一个专门的调查人员团队在自己的学科之外表现出了卓越的合作记录，从而使人们对应用新颖的遗传编码的记者策略的兴趣不断提高，从而允许在体内进行癌症的分子表征进行基础研究以及诊断和预后临床应用。特别是，这导致了一个主要的主题，以研究活生物体中调节的信号转导和蛋白质加工。主要研究项目涉及癌症中Notch信号的成像，研究代谢压力和检查点对照作为抗癌治疗的靶标，研究炎症和病毒诱导的双转基因记者小鼠中的炎症和病毒诱导的致癌性相互作用，并监测在造成良好型在造成良好型的良好过程中，对遗传学调整的细胞进行了遗传转化的细胞，从而在造成骨骼中进行了培训。该程序还包括三个分子成像科学资源：分子成像报告基因核心，化学核心和高吞吐量筛选机器人技术核心，该核心核心建立在分子成像平台上，用于读取广泛的生物测定，包括siRNA图书馆筛选，包括siRNA库筛选和化学遗传学。我们的分子成像中心还为新的研究人员提供了一个试点项目计划，并为学生，培训后和研究员提供了多学科培训计划。