Benchtop Device for Detection of BoNT in Clinical Samples

用于检测临床样品中 BoNT 的台式设备

• 批准号: 8260258
• 负责人: DOBRIN NEDELKOV
• 金额: $ 15.38万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8260258
• 关键词: Adopted; Biological; Biological Assay; Bontoxilysin; Botulinum Toxins; Calibration; Cities; Clinical; Complex; Detection; Devices; Emerging Communicable Diseases; Food; Hour; Infectious Diseases Research; Liquid substance; Optics; Organ; Peptide Hydrolases; Process; Readiness; Reading; Robot; Sampling; Serum; Solid; System; Systems Integration; Technology; Testing; Toxin; base; biodefense; biothreat; cost; improved; instrumentation; prototype

We are proposing to develop devices for the quantitative detection of botulinum neurotoxins (BoNT) at attomolar sensitivity with fully automated bench-top instrumentation. We will adopt the existing bead-based BoNT ALISSA technology, developed by the Kalkum lab at City of Hope, into a monolithic microcolumn format to be utilized by common liquid handling robots. We will also develop an automated, highly sensitive, and inexpensive wave-guide-based optical read-out system for integration in the robots. The resulting functional prototype system is expected to automatically process and analyze multiple samples simultaneously and repeatedly (up to 96 per hour). Automated calibration routines and controls for botulinum toxin and non-botulinum toxin proteases will be included. Our assay system will be tested and validated with complex biological matrices such as serum, solid organ and food extracts as well as clinical samples.

我们提议开发用于定量检测肉毒杆菌神经毒素（BoNT）的设备 全自动台式仪器具有阿摩尔级灵敏度。我们将采用现有的基于珠子的 BoNT ALISSA 技术由希望之城的 Kalkum 实验室开发成整体微柱 常见液体处理机器人使用的格式。我们还将开发一种自动化的、高度灵敏的、 以及用于集成在机器人中的廉价的基于波导的光学读出系统。由此产生的 功能原型系统有望自动处理和分析多个样品 同时和重复（每小时最多 96 个）。肉毒杆菌的自动校准程序和控制 将包括毒素和非肉毒杆菌毒素蛋白酶。我们的检测系统将经过测试和验证 复杂的生物基质，如血清、固体器官和食物提取物以及临床样品。