Detection of Respiratory Viruses in Upper and Lower Respiratory Tract Specimens

上呼吸道和下呼吸道标本中呼吸道病毒的检测

• 批准号: 8565333
• 负责人: ANTHONY F. SUFFREDINI
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8565333
• 关键词: Age; Biological Assay; Bronchoscopy; Caring; Centers for Disease Control and Prevention (U.S.); Clinical; Clinical Data; Collection; Conflict (Psychology); Data; Detection; Evolution; Female; Future; Individual; Infectious Diseases Research; Instruction; Laboratories; Lower respiratory tract structure; Medical; Medicine; Microbiology; National Institute of Allergy and Infectious Disease; Nucleic Acids; Participant; Process; Radiology Specialty; Reporting; Research; Research Personnel; Sampling; Services; Specimen; Swab; Techniques; Testing; United States National Institutes of Health; Upper respiratory tract; Viral; Viral Pathogenesis; Virus; abstracting; male; respiratory; respiratory virus

Up to 100 male or female subjects age 12 and above who are already undergoing bronchoscopy for the collection of a BAL specimen and who will have respiratory virus PCR ordered on the BAL specimen as part of their ongoing care at the NIH Clinical Center will be eligible for inclusion in this study. NP swab specimens have been validated for testing using the FilmArray assay. Both the NP swab and the BAL specimens will have the respiratory virus PCR (FilmArray) assay ordered in CRIS and the specimens will be processed and tested by the medical technologists in the Microbiology Service of the Department of Laboratory Medicine. Results from both specimens will be reported in CRIS. Instructions from the Centers for Disease Control on the proper technique for collecting NP swab specimens are presented in Appendix A. To resolve discrepant results, NP and BAL specimens with conflicting results will be tested by a second multiplex PCR (Luminex Fast RVP) in the Laboratory of Infectious Diseases, Viral Pathogenesis and Evolution Branch, NIAID. These results will only be used for research purposes. Demographic and clinical data (including but not limited to laboratory results, microbiologic data, and radiology results) on study participants will be abstracted from CRIS and correlated with results from the FilmArray assay. Excess NP swab or BAL samples will be stored for potential future use by study investigators.

多达100名12岁及以上的男性或女性已经进行了支气管镜检查，以收集BAL标本，并且将在NIH临床中心进行的持续护理中有呼吸道病毒PCR，将有资格纳入这项研究。 NP拭子标本已通过胶片测定法进行了测试验证。 NP SWAB和BAL标本都将在CRIS中订购的呼吸病毒PCR（胶片）测定法（胶片）测定法，并将由医疗技术人员在实验室医学部的微生物学服务中处理和测试。 两个标本的结果将在CRIS中报告。 疾病控制中心的指示在附录A中提供了用于收集NP拭子标本的适当技术。为了解决差异结果，具有冲突结果的NP和BAL标本，将通过第二个多重PCR（Luminex Fast RVP）在传染性疾病，病毒发病机制和进一步分支的实验室中测试。 这些结果仅用于研究目的。 研究参与者的人口统计和临床数据（包括但不限于实验室结果，微生物学数据和放射学结果）将从CRIS中抽象出来，并与电影节分析的结果相关。 多余的NP拭子或BAL样品将被存储，以便研究研究人员的未来使用。