Tissue Bank for Cellular and Molecular Studies

用于细胞和分子研究的组织库

• 批准号: 8566755
• 负责人: LAWRENCE M. WEISS
• 金额: $ 10.29万
• 依托单位: BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8566755
• 关键词: Address; Aliquot; Area; Autologous; Autologous Transplantation; Blood; Blood Cells; Blood specimen; Bone Marrow; Bone marrow biopsy; Cancer Center; Cell Line; Cells; Cities; Classification; Clinical; Collection; Complex; Consultations; Cytogenetics; Data; Data Collection; Detection of Minimal Residual Disease; Diagnosis; Ensure; Flow Cytometry; Frozen Sections; Hematopoietic; Histologic; Institutional Review Boards; Lymphoma; Malignant lymphoid neoplasm; Marrow; Molecular; Molecular Cytogenetics; Outcome; Paraffin; Pathogenesis; Pathologic; Patients; Performance; Peripheral Blood Stem Cell; Peripheral Stem Cell Transplant; Physicians; Ploidies; Population; Preparation; Process; Protein Analysis; Protocols documentation; RNA; Reproduction spores; Research; Research Personnel; Sampling; Services; Specimen; Staining method; Stains; Therapeutic; Tissue Banking; Tissue Banks; Tissue Microarray; Tissues; Transplantation; animal tissue; bone; cancer cell; cell type; follow-up; laser capture microdissection; member; peripheral blood; prospective; research clinical testing; sample collection; tissue fixing; tissue preparation; vector

The purpose of the tissue core is to facilitate research on lymphoid malignancies by members of the City of Hope Lymphoma Spore. The core provides services in the following areas; (1) acquisition and banking of fresh and paraffin-fixed tissues of lymphoma patients at City of Hope, including specimens obtained prospectively and retrospectively; (2) comprehensive work-up of lymphoma specimens to ensure correct diagnosis, classification, and detection of minimal residual disease, including immunohistochemical, flow cytometric, molecular pathologic, and cytogenetic studies; (3) performance and assistance in routine histologic processing and immunohistochemical staining of lymphoma tissues, including animal tissue, and preparations from cell lines, as well as specialized histologic services such as preparation of multitumor blocks or tissue microarrays to the specifications of researchers; (4) performance and assistance in Laser Capture microdissections from paraffin or frozen sections to obtain enrichment of specific cell types including isolation of malignant cells for analysis of proteins, RNA and/or DNA content; (5) flow cytometry studies, including complex characterization of lymphoma and host cell populations; (6) cytogenetic studies, including identification of vector-carrying cells and (7) consultation services to other SPORE investigators. In addition a Hematopoietic cell tissue bank has been established to prospectively collect and store fresh bone marrow, peripheral blood and peripheral blood stem cell samples from lymphoma patients undergoing autologous peripheral stem cell transplant (aPBSCT). This bank will support studies in Project 2 evaluating pathogenesis and predictors for t-MDS/AML in lymphoma patients post-aHCT. Samples are obtained pre-transplant and post-transplant at 100 days, 6 months, 1 year, and then annually through 5 years after transplant. Aliquots of the PBSC autograft are also banked. The core also collects and enters follow up outcome information for all patients. The core uses an IRB approved protocol for sample and data collection (see Appendix). The core also follows patients who have received their initial transplant treatment but who are not under current followup at City of Hope, by contacting the treating physician, and if necessary, recalling the patient to the City of Hope for clinical evaluation, blood draw and bone marrow biopsy

组织核心的目的是促进对淋巴恶性肿瘤的研究 希望淋巴瘤孢子。核心在以下领域提供服务； （1）收购和银行 希望市淋巴瘤患者的新鲜和石蜡固定组织，包括获得的标本 前瞻性和追溯； （2）淋巴瘤标本的全面检查以确保正确 诊断，分类和检测最小残留疾病，包括免疫组织化学，流量 细胞仪，分子病理和细胞遗传学研究； （3）常规的表现和协助 淋巴瘤组织的组织学加工和免疫组织化学染色，包括动物组织和 细胞系的准备以及专业的组织学服务，例如准备多肿块 块或组织微阵列符合研究人员的规格； （4）激光的性能和帮助 从石蜡或冷冻切片中捕获微作用，以获取特定细胞类型的富集 分离恶性细胞，以分析蛋白质，RNA和/或DNA含量； （5）流式细胞仪研究， 包括淋巴瘤和宿主细胞群体的复杂表征； （6）细胞遗传学研究，包括 鉴定载体细胞和（7）向其他孢子研究者进行咨询服务。此外 已经建立了造血细胞组织库，以预期收集和存储新鲜的骨髓， 淋巴瘤患者的外周血和外周血干细胞样品 周围干细胞移植（APBSCT）。该银行将支持项目2评估发病机理的研究 AHCT淋巴瘤患者中T-MDS/AML的预测因子。样品是在移植前获得的，并且 移植后移植后移植后移植后每年移植后5年。等分试样 PBSC自体移植也是储存的。核心还收集并输入所有人的后续结果信息 患者。核心使用IRB批准的协议进行样本和数据收集（请参阅附录）。核心 还跟随接受初步移植治疗但不接受当前随访的患者 在希望之城，通过与治疗医师联系，如有必要，将病人召回到该市 希望进行临床评估，抽血和骨髓活检