Tissue Bank for Cellular and Molecular Studies

用于细胞和分子研究的组织库

• 批准号: 6992462
• 负责人: LAWRENCE M. WEISS
• 金额: $ 10.82万
• 依托单位: BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-02 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6992462
• 关键词: Hodgkin' s disease; animal tissue; biomedical facility; bone marrow; cell biology; clinical research; hematopoietic tissue; human genetic material tag; human subject; human tissue; molecular biology; molecular genetics; nonHodgkin' s lymphoma; pathology; tissue /cell culture; tissue /cell preparation; tissue /organ preservation; tissue resource /registry

The purpose of the tissue core is to facilitate research on lymphoid malignancies by members of the City of Hope Cancer Center. It will support two tissue banks. The Pathology tissue bank core will provide services in the following areas: (1) Acquisition and banking of fresh and paraffin-fixed tissues of lymphoma patients at City of Hope, including specimens obtained prospectively and retrospectively obtaining specimens from patients previously biopsied elsewhere; (2) Comprehensive work-up of lymphoma specimens to ensure correct diagnosis and classification, including immunohistochemical, molecular pathologic, and cytogenetic studies. (3) Performance and assistance in routine histologic processing and immunohistochemical staining of lymphoma tissues, including paraffin embedding, sectioning and H&E staining of human tissue, animal tissue, and preparations from cell lines, as well as specialized histologic services such as preparation of multitumor blocks or tissue microarrays to the specifications of researchers (4) Performance and assistance in Laser Capture microdissections from paraffin or frozen sections to obtain enrichment of specific cell types including isolation of malignant cells for analysis of proteins, RNA and/or DNA content (5) Consultation services to other SPORE investigators. The Hematopoietic cell tissue bank will collect bone marrow, peripheral blood and peripheral blood stem cell samples from lymphoma patients undergoing autologous peripheral stem cell transplant (aPBSCT), and is more than 3 years old at this time. This core prospectively collects and stores fresh blood and marrow samples from patients undergoing aPBSCT for lymphoma. Samples are obtained pre-transplant and post-transplant at 100 days, 6 months, 1 year, and then annually through 5 years after transplant. In addition, aliquots of the PBSC autograft are also banked. The core also collects and enters follow up outcome information for all patients. The core uses an IRB approved protocol for sample and data collection (see Appendix). The core also follows patients who have received their initial transplant treatment but who are not under current follow-up at City of Hope, by contacting the treating physician, and if necessary, recalling the patient to the City of Hope General Clinical Research Center for clinical evaluation, blood draw and bone marrow biopsy.

组织核心的目的是促进希望之城癌症中心成员对淋巴恶性肿瘤的研究。它将支持两个组织库。病理组织库核心将在以下领域提供服务： (1) 在希望之城采集和储存淋巴瘤患者的新鲜组织和石蜡固定组织，包括前瞻性获取的标本和回顾性从以前在其他地方活检的患者获取的标本； (2)对淋巴瘤标本进行全面检查，确保正确 诊断和分类，包括免疫组织化学、分子病理学和细胞遗传学研究。 (3) 淋巴瘤组织常规组织学处理和免疫组化染色的表现和协助，包括人体组织、动物组织和细胞系制备物的石蜡包埋、切片和H&E染色，以及多肿瘤块制备等专业组织学服务或符合研究人员规格的组织微阵列 (4) 激光捕获的性能和帮助 从石蜡或冰冻切片中进行显微切割，以富集特定细胞类型，包括分离恶性细胞以分析蛋白质、RNA 和/或 DNA 含量 (5) 向其他 SPORE 研究人员提供咨询服务。造血细胞组织库将从接受自体外周干细胞移植（aPBSCT）的淋巴瘤患者收集骨髓、外周血和外周血干细胞样本，此时年龄已超过3岁。该核心前瞻性地收集和储存接受 aPBSCT 治疗淋巴瘤的患者的新鲜血液和骨髓样本。移植前获取样本 移植后 100 天、6 个月、1 年，然后每年一次，直至移植后 5 年。此外，PBSC 自体移植物的等分试样也被储存。该核心还收集并输入所有患者的随访结果信息。核心使用 IRB 批准的样本和数据收集协议（参见附录）。该核心还跟踪那些已接受初始移植治疗但当前未在 City of Hope 进行随访的患者，联系治疗医生，并在必要时将患者召回至 City of Hope 综合临床研究中心进行临床治疗。评估、抽血和骨髓活检。