Tissue Bank for Cellular and Molecular Studies

用于细胞和分子研究的组织库

基本信息

批准号：
8555443
负责人：
LAWRENCE M. WEISS
金额：
$ 8.78万
依托单位：
BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-09-02 至 2016-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8555443
关键词：
Address Aliquot Area Autologous Autologous Transplantation Blood Blood Cells Blood specimen Bone Marrow Bone marrow biopsy Cancer Center Cell Line Cells Cities Classification Clinical Collection Complex Consultations Cytogenetics Data Data Collection Detection of Minimal Residual Disease Diagnosis Ensure Flow Cytometry Frozen Sections Hematopoietic Histologic Institutional Review Boards Lymphoma Malignant lymphoid neoplasm Marrow Molecular Molecular Cytogenetics Outcome Paraffin Pathogenesis Pathologic Patients Performance Peripheral Blood Stem Cell Peripheral Stem Cell Transplant Physicians Ploidies Population Preparation Process Protein Analysis Protocols documentation RNA Reproduction spores Research Research Personnel Sampling Services Specimen Staining method Stains Therapeutic Tissue Banking Tissue Banks Tissue Microarray Tissues Transplantation animal tissue bone cancer cell cell type follow-up laser capture microdissection member peripheral blood prospective research clinical testing sample collection tissue fixing tissue preparation vector

项目摘要

The purpose of the tissue core is to facilitate research on lymphoid malignancies by members of the City of Hope Lymphoma Spore. The core provides services in the following areas; (1) acquisition and banking of fresh and paraffin-fixed tissues of lymphoma patients at City of Hope, including specimens obtained prospectively and retrospectively; (2) comprehensive work-up of lymphoma specimens to ensure correct diagnosis, classification, and detection of minimal residual disease, including immunohistochemical, flow cytometric, molecular pathologic, and cytogenetic studies; (3) performance and assistance in routine histologic processing and immunohistochemical staining of lymphoma tissues, including animal tissue, and preparations from cell lines, as well as specialized histologic services such as preparation of multitumor blocks or tissue microarrays to the specifications of researchers; (4) performance and assistance in Laser Capture microdissections from paraffin or frozen sections to obtain enrichment of specific cell types including isolation of malignant cells for analysis of proteins, RNA and/or DNA content; (5) flow cytometry studies, including complex characterization of lymphoma and host cell populations; (6) cytogenetic studies, including identification of vector-carrying cells and (7) consultation services to other SPORE investigators. In addition a Hematopoietic cell tissue bank has been established to prospectively collect and store fresh bone marrow, peripheral blood and peripheral blood stem cell samples from lymphoma patients undergoing autologous peripheral stem cell transplant (aPBSCT). This bank will support studies in Project 2 evaluating pathogenesis and predictors for t-MDS/AML in lymphoma patients post-aHCT. Samples are obtained pre-transplant and post-transplant at 100 days, 6 months, 1 year, and then annually through 5 years after transplant. Aliquots of the PBSC autograft are also banked. The core also collects and enters follow up outcome information for all patients. The core uses an IRB approved protocol for sample and data collection (see Appendix). The core also follows patients who have received their initial transplant treatment but who are not under current followup at City of Hope, by contacting the treating physician, and if necessary, recalling the patient to the City of Hope for clinical evaluation, blood draw and bone marrow biopsy

组织核心的目的是促进城市成员对淋巴恶性肿瘤的研究希望淋巴瘤孢子。核心提供以下领域的服务； (1) 收购和银行业务希望之城淋巴瘤患者的新鲜组织和石蜡固定组织，包括获得的标本前瞻性和回顾性； (2)对淋巴瘤标本进行全面检查，确保正确微小残留病的诊断、分类和检测，包括免疫组织化学、流式细胞术细胞计数、分子病理学和细胞遗传学研究； (3)日常表现及协助淋巴瘤组织（包括动物组织）的组织学处理和免疫组织化学染色，以及细胞系制剂，以及专门的组织学服务，例如多肿瘤的制备符合研究人员规格的块或组织微阵列； (4) 激光的表现与协助从石蜡或冷冻切片中捕获显微解剖，以获得特定细胞类型的富集，包括分离恶性细胞以分析蛋白质、RNA 和/或 DNA 含量； (5)流式细胞术研究，包括淋巴瘤和宿主细胞群的复杂特征； (6)细胞遗传学研究，包括载体携带细胞的鉴定以及 (7) 为其他 SPORE 研究人员提供咨询服务。此外已建立造血细胞组织库，前瞻性收集和储存新鲜骨髓，接受自体移植的淋巴瘤患者的外周血和外周血干细胞样本外周干细胞移植（aPBSCT）。该银行将支持项目 2 评估发病机制的研究以及 aHCT 后淋巴瘤患者 t-MDS/AML 的预测因子。移植前获取样本并移植后 100 天、6 个月、1 年，然后每年一次，直至移植后 5 年。等分试样 PBSC 自体移植物也被储存。核心还收集并输入所有后续结果信息患者。核心使用 IRB 批准的样本和数据收集协议（参见附录）。核心还跟踪已接受初始移植治疗但目前未接受随访的患者在希望之城，联系主治医生，如有必要，将患者召回希望之城希望进行临床评估、抽血和骨髓活检