A Novel Multilayered Membrane for Periodontal Regeneration

用于牙周再生的新型多层膜

基本信息

批准号：
8565199
负责人：
Marco C Bottino
金额：
$ 14.31万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-07-01 至 2018-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8565199
关键词：
Adhesions Adult Affect Alkaline Phosphatase Alveolar Award Biocompatible Materials Biology Bone Density Bone Regeneration Bone Surface Bone Tissue Canis familiaris Cell Count Cell Culture Techniques Cell Survival Cellular biology Cementoblast Chronic Clinical Clinical Trials Connective Tissue Cues Defect Dental Dental Cementum Dentists Development Development Plans Disease Effectiveness Environment Epithelial Attachment Evaluation Extracellular Matrix Proteins FGF2 gene Fibroblast Growth Factor Fibroblast Growth Factor 2 Fibroblasts Foundations Funding Goals Gray unit of radiation dose Growth and Development function Guided Tissue Regeneration Height Histocompatibility Testing Human Image Implant In Vitro Inflammatory Knowledge Laboratories Lead Left Literature Location Mandible Measurement Measures Membrane Mentors Mesenchymal Stem Cells Methodology Methods Metric Modeling Molecular Biology Molecular Profiling Molecular and Cellular Biology Nanotechnology Natural regeneration Operative Surgical Procedures Oral Osteogenesis Patient Care Periodontal Ligament Periodontitis Polytetrafluoroethylene Positioning Attribute Principal Investigator Procedures Qualifying Research Resources Scientist Secure Side Signal Transduction Stem cells Supervision Surface Techniques Tissue Engineering Tissues Tooth Loss Tooth root structure Tooth structure Training Translating Translational Research aging population alveolar bone biomaterial development bone calcium phosphate career development clinical practice craniofacial design implantation in vitro Assay in vivo innovation migration mineralization multidisciplinary novel osteoblast differentiation programs public health relevance regenerative regenerative therapy research study response skeletal skills socioeconomics soft tissue tissue reconstruction tissue regeneration

项目摘要

DESCRIPTION (provided by applicant): With the US population aging, it is important to find novel dental biomaterials that will promote periodontal regeneration, and translate these to clinical practice. As both a dentist and materials scientist, the applicant is in a position to brige the gap between biomaterials development. and their application in patient care using nanotechnology, tissue engineering and clinical knowledge. The purpose of this award is to provide the principal investigator extensive training in cellular and molecular biology, skeletal biology, and translational research with an emphasis on periodontal tissue regeneration. The experiments in this application have been designed to incorporate new methodologies, and will be guided by a multidisciplinary team of experts who will provide the required training to advance Dr. Bottino's scientific technical and analytic skills. The principal investigator's ultimae goal is to become a productive and independent clinician-scientist who can secure federal funding to sponsor an independent research program. The central hypothesis of the proposed research is that a multilayer membrane capable of stimulating the regeneration of various tissue types in a location specific manner, through the addition of calcium phosphate and fibroblast growth factor-2 (CaP/FGF2) and FGF2, will lead to alveolar and periodontal tissue regeneration in the appropriate locations. The literature indicates that no single implantable biomaterial can consistently guide the coordinated growth and development of multiple tissue types, especially in very large periodontal defects. Thus, there is a critical need to develop regenerative therapies to promote oral and craniofacial tissue regeneration and reconstruction. The specific aims of the proposed research are (1) To use an in vitro assay model to maximize the tissue-specific regenerative capacity of the multilayered implant surface layers, (2) To assess the effectiveness of CaP/FGF2 on bone regeneration in an alveolar defect using an in vivo canine model, and (3) To assess the effectiveness of the multilayered implant to regenerate soft and mineralized tissues in a location-specific manner using an in vivo canine model. The principal investigator has devised a comprehensive career development plan that includes formal coursework, laboratory guidance to cover intensive cell and molecular biology training, as well as in vivo techniques combined with a diverse set of bone/periodontal tissue regeneration related analyses under the supervision of his mentoring team. Collectively, the K08 award has the capacity to provide the resources and training needed to give the applicant the foundation and critical theoretical and practical means to become a highly qualified clinician-scientist who can translate his knowledge of biomaterials to clinical practice.

描述（由申请人提供）：随着美国人口的衰老，找到新型的牙科生物材料很重要，这些牙科生物材料将促进牙周再生，并将其转化为临床实践。作为牙医和材料科学家，申请人可以弥补生物材料开发之间的差距。以及它们在患者护理中使用纳米技术，组织工程和临床知识的应用。该奖项的目的是为细胞和分子生物学，骨骼生物学和翻译研究提供主要研究者的广泛培训，重点是牙周组织再生。本应用程序中的实验旨在纳入新的方法，并将在一个多学科的专家团队的指导下，他们将提供所需的培训，以提高Bottino博士的科学技术和分析技能。首席研究者的最后一个目标是成为一名富有成效的独立临床医生科学家，他可以确保联邦资金赞助独立研究计划。拟议研究的中心假设是，通过添加磷酸钙和成纤维细胞生长因子2（CAP/FGF2）和FGF2，能够以特定位置方式刺激各种组织类型的再生的多层膜将导致适当位置的牙槽和牙周组织再生。文献表明，没有一种可植入的生物材料可以始终如一地指导多种组织类型的协调生长和发展，尤其是在非常大的牙周缺陷中。因此，迫切需要开发再生疗法促进口腔和颅面组织再生和重建。拟议研究的具体目的是（1）使用体外测定模型来最大化多层植入物表面层的组织特异性再生能力，（2）评估CAP/FGF2对肺泡再生的有效性使用体内犬模型的缺陷，（3）使用体内犬模型评估多层植入物以特定于位置的方式再生软和矿化组织的有效性。首席研究人员已经制定了一项全面的职业发展计划，其中包括正式课程，实验室指导，涵盖密集细胞和分子生物学培训，以及在他的体内技术，以及在其监督下与骨/牙周组织再生相关的各种相关分析的多样化技术。指导团队。总的来说，K08奖具有提供所需的资源和培训的能力，以使申请人成为基础和关键的理论和实践手段，以成为一名高素质的临床医生，他们可以将他的生物材料知识转化为临床实践。