Tectal influences on visual thalamic activity: an optogenetic approach

顶盖对视觉丘脑活动的影响：光遗传学方法

• 批准号: 8401678
• 负责人: WILLIAM GUIDO
• 金额: $ 20.5万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-30 至 2014-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8401678
• 关键词: Action Potentials; Acute; Address; Affect; Area; Behavior; Cations; Cell Nucleus; Cells; Chimera organism; Chloride Ion; Chlorides; Data; Depressed mood; Dorsal; Electric Stimulation; Electrodes; Eye; Fiber; Fiber Optics; Glutamates; Halorhodopsins; Head Movements; Imagery; In Vitro; Lateral Geniculate Body; Lead; Light; Location; Mammals; Mus; Mutation; Neurons; Optic tract structure; Pathway interactions; Pattern; Peripheral; Physiologic pulse; Process; Property; Pump; Reporter; Retina; Retinal; Retinal Ganglion Cells; Route; Sense Organs; Sensory; Shapes; Signal Transduction; Slice; Source; Space Perception; Structure; Synapses; Techniques; Tectum Mesencephali; Thalamic structure; Transgenic Mice; Variant; Viral Vector; Visual; Visual Cortex; Whole-Cell Recordings; adeno-associated viral vector; biocytin; cell type; extracellular; image processing; in vivo; information processing; insight; loss of function; nerve supply; nervous system disorder; object recognition; optogenetics; polypeptide; postsynaptic; receptive field; recombinase; red fluorescent protein; research study; response; retinogeniculate; retinotectal; superior colliculus Corpora quadrigemina; tool; transmission process; vector; visual information; visual stimulus

DESCRIPTION (provided by applicant): Much is known about how inputs from sources other than the retina affect the thalamic relay of retinal signals in route to the visual cortex. They arse from a variety of subcortical and cortical sources, comprise the bulk of synapses onto thalamic relay cells and act as modulators to affect the gain of retinogeniculate signal transmission. The superior collliculus (SC), a primary retino-recipient structure, also sends a prominent projection to the dorsal lateral geniculate nucleus (dLGN), the first order nucleus that conveys retinal signals to visual cortex. However, whether SC input acts as a driver to help shape the receptive field structure of dLGN relay neurons or as a modulator to dampen or amplify the flow of thalamic information remains untested. To understand how information arising from the SC influences dLGN function and how particular SC cell types contribute to this, we shall make use of mouse transgenics to visualize or target cell types and projections of the tectogeniculate pathway, optogenetics to photo-activate SC inputs to evoke postsynaptic activity in dLGN cells, and in vitro and in vivo recording techniques to assess whether SC inputs behave as drivers or modulators of dLGN activity. These combined approaches will allow us to selectively control the neuronal activity of the tecto-geniculate pathway and shed light on how interactions between two retino- recipient subcortical structures affect the processing and transfer of visual information. PUBLIC HEALTH RELEVANCE: These studies will provide valuable information about how first-order sensory nuclei of the thalamus receive and process information from peripheral sense organs. They shall also reveal how afferent input from central structures can modulate the flow of thalamic information in route to sensory cortical areas. Understanding the functional and structural organization of thalamic circuitry with optogenetic tools may offer further insight into the study and treatment of neurological disorders that result from the formation of abnormal patterns of connectivity.

描述（由申请人提供）：关于视网膜以外的其他来源的输入如何影响视网膜信号的丘脑通往视觉皮层的丘脑继电器。它们来自各种皮层和皮质来源，包括大部分突触到丘脑中继细胞上，并充当调节剂，以影响视网膜生成信号传递的增益。上级视网膜 - 接收器结构上的上胶囊（SC）也向背侧侧向基因核（DLGN）发送了突出的投影，这是将视网膜信号传达到视觉皮层的一阶核。但是，SC输入是有助于塑造DLGN继电器神经元的接受场结构的驱动因素还是作为调节剂来抑制或放大丘脑信息的流动。要了解SC产生的信息如何影响DLGN功能以及特定的SC细胞类型对此有何贡献，我们将利用鼠标转基因来可视化或靶向构成途径的细胞类型或靶向细胞类型和投影，以光质估算的光质学以光激活SC输入以唤起dlgn细胞中的dlgn细胞和模式的vivo和vivo condectors of vistor and vivo recture and vivo condection是否在vivo中进行了动态。 活动。这些合并的方法将使我们能够选择性地控制Tecto生成途径的神经元活性，并阐明两个视网膜皮层下皮层结构之间的相互作用如何影响视觉信息的处理和传递。 公共卫生相关性：这些研究将提供有关丘脑的一阶感觉核如何从外围感官器官接收和处理信息的宝贵信息。他们还应揭示中央结构的传入输入如何调节丘脑信息在进入感觉皮质区域的途径。了解使用光遗传学工具的丘脑电路的功能和结构组织可能会进一步了解 由异常连通性模式形成导致的神经系统疾病的研究和治疗。