Tectal influences on visual thalamic activity: an optogenetic approach

顶盖对视觉丘脑活动的影响：光遗传学方法

基本信息

批准号：
8401678
负责人：
WILLIAM GUIDO
金额：
$ 20.5万
依托单位：
UNIVERSITY OF LOUISVILLE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-30 至 2014-09-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8401678
关键词：
Action Potentials Acute Address Affect Area Behavior Cations Cell Nucleus Cells Chimera organism Chloride Ion Chlorides Data Depressed mood Dorsal Electric Stimulation Electrodes Eye Fiber Fiber Optics Glutamates Halorhodopsins Head Movements Imagery In Vitro Lateral Geniculate Body Lead Light Location Mammals Mus Mutation Neurons Optic tract structure Pathway interactions Pattern Peripheral Physiologic pulse Process Property Pump Reporter Retina Retinal Retinal Ganglion Cells Route Sense Organs Sensory Shapes Signal Transduction Slice Source Space Perception Structure Synapses Techniques Tectum Mesencephali Thalamic structure Transgenic Mice Variant Viral Vector Visual Visual Cortex Whole-Cell Recordings adeno-associated viral vector biocytin cell type extracellular image processing in vivo information processing insight loss of function nerve supply nervous system disorder object recognition optogenetics polypeptide postsynaptic receptive field recombinase red fluorescent protein research study response retinogeniculate retinotectal superior colliculus Corpora quadrigemina tool transmission process vector visual information visual stimulus

项目摘要

DESCRIPTION (provided by applicant): Much is known about how inputs from sources other than the retina affect the thalamic relay of retinal signals in route to the visual cortex. They arse from a variety of subcortical and cortical sources, comprise the bulk of synapses onto thalamic relay cells and act as modulators to affect the gain of retinogeniculate signal transmission. The superior collliculus (SC), a primary retino-recipient structure, also sends a prominent projection to the dorsal lateral geniculate nucleus (dLGN), the first order nucleus that conveys retinal signals to visual cortex. However, whether SC input acts as a driver to help shape the receptive field structure of dLGN relay neurons or as a modulator to dampen or amplify the flow of thalamic information remains untested. To understand how information arising from the SC influences dLGN function and how particular SC cell types contribute to this, we shall make use of mouse transgenics to visualize or target cell types and projections of the tectogeniculate pathway, optogenetics to photo-activate SC inputs to evoke postsynaptic activity in dLGN cells, and in vitro and in vivo recording techniques to assess whether SC inputs behave as drivers or modulators of dLGN activity. These combined approaches will allow us to selectively control the neuronal activity of the tecto-geniculate pathway and shed light on how interactions between two retino- recipient subcortical structures affect the processing and transfer of visual information. PUBLIC HEALTH RELEVANCE: These studies will provide valuable information about how first-order sensory nuclei of the thalamus receive and process information from peripheral sense organs. They shall also reveal how afferent input from central structures can modulate the flow of thalamic information in route to sensory cortical areas. Understanding the functional and structural organization of thalamic circuitry with optogenetic tools may offer further insight into the study and treatment of neurological disorders that result from the formation of abnormal patterns of connectivity.

描述（由申请人提供）：关于来自视网膜以外的来源的输入如何影响视网膜信号到视觉皮层的丘脑中继，人们已经了解很多。它们来自各种皮质下和皮质来源，包含丘脑中继细胞上的大部分突触，并充当调节器来影响视网膜信号传输的增益。上丘（SC）是主要的视网膜接受结构，也向背外侧膝状核（dLGN）发送突出的投影，dLGN是向视觉皮层传递视网膜信号的一级核。然而，SC 输入是否充当驱动器来帮助塑造 dLGN 中继神经元的感受野结构，或者充当调节器来抑制或放大丘脑信息流，仍有待测试。为了了解 SC 产生的信息如何影响 dLGN 功能以及特定的 SC 细胞类型如何对此做出贡献，我们将利用小鼠转基因来可视化或靶向细胞类型以及 tectogeniculate 途径的预测，利用光遗传学来光激活 SC 输入以唤起dLGN 细胞中的突触后活性，以及体外和体内记录技术来评估 SC 输入是否充当 dLGN 活性的驱动程序或调节剂。这些组合方法将使我们能够选择性地控制顶盖通路的神经元活动，并揭示两个视网膜受体皮层下结构之间的相互作用如何影响视觉信息的处理和传递。公共健康相关性：这些研究将提供有关丘脑一级感觉核如何接收和处理来自周围感觉器官的信息的宝贵信息。他们还应揭示来自中央结构的传入输入如何调节丘脑信息流到感觉皮层区域。使用光遗传学工具了解丘脑回路的功能和结构组织可能会提供进一步的见解对因异常连接模式形成而导致的神经系统疾病的研究和治疗。