Tectal influences on visual thalamic activity: an optogenetic approach

顶盖对视觉丘脑活动的影响：光遗传学方法

基本信息

批准号：
8549257
负责人：
WILLIAM GUIDO
金额：
$ 17.81万
依托单位：
UNIVERSITY OF LOUISVILLE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-30 至 2015-09-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8549257
关键词：
Action Potentials Acute Address Affect Area Behavior Cations Cell Nucleus Cells Chimera organism Chloride Ion Chlorides Data Depressed mood Dorsal Electric Stimulation Electrodes Eye Fiber Fiber Optics Glutamates Halorhodopsins Head Movements Imagery In Vitro Lateral Geniculate Body Lead Light Location Mammals Mus Mutation Neurons Optic tract structure Pathway interactions Pattern Peripheral Physiologic pulse Process Property Pump Reporter Retina Retinal Retinal Ganglion Cells Route Sense Organs Sensory Shapes Signal Transduction Slice Source Space Perception Structure Synapses Techniques Tectum Mesencephali Thalamic structure Transgenic Mice Variant Viral Vector Visual Visual Cortex Whole-Cell Recordings adeno-associated viral vector biocytin cell type extracellular image processing in vivo information processing insight loss of function nerve supply nervous system disorder object recognition optogenetics polypeptide postsynaptic receptive field recombinase red fluorescent protein research study response retinogeniculate retinotectal superior colliculus Corpora quadrigemina tool transmission process vector visual information visual stimulus

项目摘要

DESCRIPTION (provided by applicant): Much is known about how inputs from sources other than the retina affect the thalamic relay of retinal signals in route to the visual cortex. They arse from a variety of subcortical and cortical sources, comprise the bulk of synapses onto thalamic relay cells and act as modulators to affect the gain of retinogeniculate signal transmission. The superior collliculus (SC), a primary retino-recipient structure, also sends a prominent projection to the dorsal lateral geniculate nucleus (dLGN), the first order nucleus that conveys retinal signals to visual cortex. However, whether SC input acts as a driver to help shape the receptive field structure of dLGN relay neurons or as a modulator to dampen or amplify the flow of thalamic information remains untested. To understand how information arising from the SC influences dLGN function and how particular SC cell types contribute to this, we shall make use of mouse transgenics to visualize or target cell types and projections of the tectogeniculate pathway, optogenetics to photo-activate SC inputs to evoke postsynaptic activity in dLGN cells, and in vitro and in vivo recording techniques to assess whether SC inputs behave as drivers or modulators of dLGN activity. These combined approaches will allow us to selectively control the neuronal activity of the tecto-geniculate pathway and shed light on how interactions between two retino- recipient subcortical structures affect the processing and transfer of visual information.

描述（由申请人提供）：关于来自视网膜以外的来源的输入如何影响视网膜信号到视觉皮层的丘脑中继，人们已经了解很多。它们来自各种皮质下和皮质来源，包含丘脑中继细胞上的大部分突触，并充当调节器来影响视网膜信号传输的增益。上丘（SC）是主要的视网膜接受结构，也向背外侧膝状核（dLGN）发送突出的投影，dLGN是向视觉皮层传递视网膜信号的一级核。然而，SC 输入是否充当驱动器来帮助塑造 dLGN 中继神经元的感受野结构，或者充当调节器来抑制或放大丘脑信息流，仍有待测试。为了了解 SC 产生的信息如何影响 dLGN 功能以及特定的 SC 细胞类型如何对此做出贡献，我们将利用小鼠转基因来可视化或靶向细胞类型以及 tectogeniculate 途径的预测，利用光遗传学来光激活 SC 输入以唤起dLGN 细胞中的突触后活性，以及体外和体内记录技术来评估 SC 输入是否充当 dLGN 活性的驱动程序或调节剂。这些组合方法将使我们能够选择性地控制顶盖通路的神经元活动，并揭示两个视网膜受体皮层下结构之间的相互作用如何影响视觉信息的处理和传递。