Mentored Quantitative Research Career Development Award

指导定量研究职业发展奖

• 批准号: 8046198
• 负责人: Z. Hugh Fan
• 金额: $ 17.94万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-10 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8046198
• 关键词: Acute Lymphocytic Leukemia; Acute leukemia; Adult; Aftercare; American; Antibodies; Award; Base Sequence; Benchmarking; Binding; Biology; Biomedical Engineering; Biomedical Research; Blast Cell; Blood; Blood Cells; Blood Volume; Blood specimen; Bone Marrow; Cancer Biology; Cancer Relapse; Cause of Death; Cells; Cessation of life; Clinical; Compact discs; Cost Analysis; Detection; Detection of Minimal Residual Disease; Devices; Diagnosis; Diagnostic; Diagnostic Neoplasm Staging; Disease; Disease remission; Early treatment; Engineering; Ensure; Environment; European; Evaluation; Flow Cytometry; Genetic; Goals; Guidelines; Hematopoietic Stem Cell Transplantation; Immunophenotyping; K-Series Research Career Programs; Knowledge; Laboratories; Lead; Malignant - descriptor; Malignant Neoplasms; Medical; Medicine; Mentors; Methods; Microfluidics; Monitor; Nanotechnology; Nucleic acid sequencing; Outcome; Patients; Phenotype; Plastics; Polymerase Chain Reaction; Population; Prevention; Reagent; Reporting; Research; Residual Neoplasm; Residual state; Rotation; Sampling; Science; Screening for cancer; Solutions; Sorting - Cell Movement; Surface; Symptoms; Technology; United States; Validation; aptamer; base; cancer cell; cancer prevention; clinical care; design; experience; improved; innovation; leukemia; manufacturing process; milliliter; neoplastic cell; outcome forecast; peripheral blood; research and development; skills; treatment response

DESCRIPTION (provided by applicant): This "Mentored Quantitative Research Development Award" will facilitate the candidate, an engineer, to become a biologist. It will be achieved by taking courses to gain fundamental knowledge in biology/medicine and developing relevant research skills via mentored research. The selected courses will include core courses encompassing the fundamentals of biomedical sciences and advanced courses. Biomedical research experience will be obtained through lab rotations in the mentor's and co-mentor's laboratories. The mentored research is to develop a platform to concentrate and enrich acute leukemia cells for detection of minimal residual disease (MRD). MRD refers to the presence of residual malignant cells that remain in the patient during treatment or after treatment when the patient is in remission (no symptoms or signs of disease). The current benchmark method is not sufficiently sensitive for detecting all remaining tumor cells in peripheral blood. As a result, a platform with a higher sensitivity will be desirable since MRD can distinguish those who need intensive and potentially more toxic therapy from those who do not. The specific aims of this project are to (1) design and fabricate a device for enrichment of leukemia cells; (2) demonstrate the enrichment of leukemia cells in the device using aptamers with specific binding to acute lymphoblastic leukemia cells; and (3) validate the platform technology by analyzing leukemia cells in clinical samples and detect MRD. The significance of the research lies in the following aspects. First, the proposed research will lead to a platform for a rapid and efficient enrichment of acute leukemia cells. The platform will offer a measureable approach to determine if any additional chemo-therapy is needed; this will bring significant benefits to leukemia patients since unnecessary toxic therapy can be avoided. Second, the platform can be easily adapted for enrichment of rare circulating cells of other cancers. Isolation of these rare tumor cells could offer an opportunity for early-stage cancer screening and medical diagnostics. Third, the proposed research will advance the fields of cancer biology, biomedical engineering, and micro-nanotechnologies. PUBLIC HEALTH RELEVANCE: Cancer is one of the leading causes of death hence innovative platforms that would accelerate the research and understanding of cancer biology could have significant societal impacts. The goal of the proposed research is to develop a platform for detecting minimal residual disease of acute leukemia, which can effectively guide clinical care and increase the cure rate.

描述（由申请人提供）：该“指导定量研究开发奖”将促进候选人（工程师）成为生物学家。这将通过学习课程来获得生物学/医学的基础知识并通过指导研究培养相关的研究技能来实现。所选课程将包括生物医学基础的核心课程和高级课程。生物医学研究经验将通过导师和共同导师实验室的实验室轮换获得。指导的研究是开发一个平台来浓缩和富集急性白血病细胞，以检测微小残留病（MRD）。 MRD是指在治疗期间或治疗后当患者处于缓解期（无疾病症状或体征）时，患者体内残留的残留恶性细胞的存在。目前的基准方法对于检测外周血中所有剩余的肿瘤细胞不够灵敏。因此，我们需要一个具有更高灵敏度的平台，因为 MRD 可以区分那些需要强化且可能毒性更强的治疗的患者与不需要的患者。该项目的具体目标是（1）设计和制造白血病细胞富集装置； (2) 证明使用与急性淋巴细胞白血病细胞特异性结合的适体对装置中的白血病细胞进行富集； (3)通过分析临床样本中的白血病细胞并检测MRD来验证平台技术。研究的意义在于以下几个方面。首先，拟议的研究将建立一个快速有效富集急性白血病细胞的平台。该平台将提供一种可测量的方法来确定是否需要任何额外的化疗；这将为白血病患者带来显着的好处，因为可以避免不必要的毒性治疗。其次，该平台可以轻松适应其他癌症的稀有循环细胞的富集。分离这些罕见的肿瘤细胞可以为早期癌症筛查和医学诊断提供机会。第三，拟议的研究将推动癌症生物学、生物医学工程和微纳米技术领域的发展。 公共健康相关性：癌症是导致死亡的主要原因之一，因此加速癌症生物学研究和理解的创新平台可能会产生重大的社会影响。本研究的目标是开发一个检测急性白血病微小残留病的平台，可以有效指导临床护理并提高治愈率。