Mass Spectrometry and Proteomics Shared Resouce

质谱和蛋白质组学共享资源

• 批准号: 8465119
• 负责人: CHAD C NELSON
• 金额: $ 4.77万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8465119
• 关键词: Advisory Committees; Budgets; Cancer Center; Cancer Center Support Grant; Cell Differentiation process; Cells; Chad; Communities; Consultations; DNA amplification; Developmental Therapeutics Program; Diagnostic Imaging; Dissociation; Doctor of Philosophy; Drug Delivery Systems; Electrons; Equipment; Faculty; Fee-for-Service Plans; Fourier Transform; Funding; Goals; Hybrids; Institutes; Laboratories; Leadership; Malignant Neoplasms; Malignant neoplasm of gastrointestinal tract; Mass Spectrum Analysis; Modeling; NCI Center for Cancer Research; Natural Product Drug; Nuclear; Nucleic Acids; Peer Review; Peptides; Pharmaceutical Preparations; Play; Polymers; Post-Translational Protein Processing; Proteins; Proteomics; Proteomics Shared Resource; Regulation; Research; Resource Sharing; Resources; Role; Services; Structure; Supervision; Surveys; Therapeutic; Training; Universities; Utah; base; cell growth; instrument; instrumentation; mass spectrometer; member; programs; protein aminoacid sequence; response; satisfaction

The Mass Spectrometry and Proteomics (MSP) Shared Resource provides Huntsman Cancer Institute (HCI) members and the entire University of Utah research community a broad range of mass spectrometry services, including state-of-the-art instrumentation, consultation, and expertise, particularly for proteomics-based research. The hybrid Fourier-Transform mass spectrometer (model LTQ-FT, ThermoElectron) is the main proteomics instrument. It provides protein identification (ID) at levels as low as 50 attomole, high-mass accuracy (<2ppm), and peptide sequencing by electron-capture dissociation (ECD), infrared multiphoton dissociation (IRMPD), and collision-induced dissociation (CID). Proteomics services include protein ID; characterization of post-translational modifications (e.g., phosphorylafion); and analysis of intact proteins. The Resource also provides mass spectrometry services for a wide range of other research applications such as structural analysis of natural products, drugs and drug-polymer conjugates, nucleic acids, DNA amplification products (e.g., PCR), large biomolecules and polymers, peptides, and small synthetic molecules. Chad Nelson, PhD, has directed the MSP Shared Resource since 2003, with the assistance of two staff members. The instrumentation and staff of the MSP Shared Resource are centrally located. The MSP Shared Resource serves all HCI laboratory-based scientific Programs (Nuclear Control of Cell Growth and Differentiation; Cell Response and Regulation; Imaging, Diagnostics, and Therapeutics; and Gastrointestinal Cancers), characterizing molecules, particularly proteins and post-translational modifications, that play roles in cancer mechanisms. In addition, characterization of small compounds by the MSP Shared Resource, including natural products and drug-delivery polymers, contributes significantly to the developmental therapeutics activities of HCI. The MSP Shared Resource is an institutionally managed facility with supervision from both University and HCI leadership. There is a Faculty Advisory Committee and the Resource is reviewed for user satisfaction by annual surveys. The facility is operated on a fee-for-service basis (chargeback). In 2008, overall use of the MSP Shared Resource by Cancer Center members with peer-reviewed funding and Cancer Center Shared Resources was 31 percent. Funds are requested from the CCSG to cover 14 percent ($44,857) of the proposed Resource budget. The MSP Shared Resource has ample capacity for additional use by Cancer Center members.

质谱和蛋白质组学 (MSP) 共享资源为亨斯迈癌症研究所 (HCI) 成员和整个犹他大学研究界提供广泛的质谱服务，包括最先进的仪器、咨询和专业知识，特别是用于基于蛋白质组学的研究。混合傅里叶变换质谱仪（型号 LTQ-FT，ThermoElectron）是主要的蛋白质组学仪器。它提供低至 50 attomole 水平的蛋白质鉴定 (ID)、高质量准确度 (<2ppm) 以及通过电子捕获解离 (ECD)、红外多光子解离 (IRMPD) 和碰撞诱导解离 (CID) 进行的肽测序）。蛋白质组学服务包括蛋白质鉴定；翻译后修饰的表征（例如磷酸化）；和完整蛋白质的分析。该资源还为广泛的其他研究应用提供质谱服务，例如天然产物、药物和药物聚合物缀合物、核酸、DNA 扩增产品（例如 PCR）、大生物分子和聚合物、肽和药物的结构分析。合成小分子。 Chad Nelson 博士自 2003 年起在两名工作人员的协助下负责管理 MSP 共享资源。 MSP 共享资源的仪器和工作人员位于中心位置。 MSP 共享资源服务于所有基于 HCI 实验室的科学项目（细胞生长和分化的核控制；细胞反应和调节；成像、诊断和治疗；以及胃肠道癌症），表征分子，特别是蛋白质和翻译后修饰，在癌症机制中发挥作用。此外，MSP 共享资源对小化合物（包括天然产物和药物输送聚合物）的表征，对 HCI 的开发治疗活动做出了重大贡献。 MSP 共享资源是一个机构化管理的设施，受到大学和 HCI 领导层的监督。有一个教师咨询委员会，并通过年度调查来审查该资源的用户满意度。该设施按服务收费（退款）运营。 2008 年，癌症中心成员通过同行评审资金和癌症中心共享资源对 MSP 共享资源的总体使用率为 31%。向 CCSG 请求资金来支付拟议资源预算的 14%（44,857 美元）。 MSP 共享资源有足够的容量供癌症中心成员额外使用。