Laboratory Sciences Core Research Plan (D)

实验室科学核心研究计划（D）

• 批准号: 8465180
• 负责人: Savita Pahwa
• 金额: $ 32.93万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8465180
• 关键词: AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Advisory Committees; Area; B-Lymphocytes; Back; Basic Science; Behavioral; Bioinformatics; Biological Assay; Biology; CD4 Lymphocyte Count; Calculi; Cell Lineage; Cells; Charge; Clinical; Clinical Protocols; Clinical Sciences; Clinical Trials; Collaborations; Community Outreach; Consultations; Cryopreservation; Data Analyses; Dendritic Cells; Development; Diagnostic; Education; Educational Activities; Experimental Designs; Faculty; Flow Cytometry; Focus Groups; Genes; Genomics; Goals; Grant; Group Meetings; Guidelines; HIV; HIV Seropositivity; Human; Immunologic Monitoring; Immunologics; Immunology; Immunology procedure; Industry; Institutes; Investigation; Journals; Laboratories; Leadership; Lentivirus Vector; Link; Molecular Virology; Monitor; Myelogenous; Myeloid Cells; Neuropathogenesis; Pathogenesis; Population; Prevention; Process; Protocols documentation; Public Health; Publications; RNA Interference; Recommendation; Recruitment Activity; Research; Research Personnel; Resources; Role; SIV; Safety; Sampling; Science; Scientist; Series; Services; Systems Biology; Thymus Gland; Training; Transfection; Transportation; Universities; Vaccine Research; Vaccine Therapy; Viral load measurement; Virus; Work; base; behavioral/social science; biobank; cohort; cytokine; enzyme linked immunospot assay; experience; gene therapy; innovation; macrophage; member; microbial; monocyte; new technology; nonhuman primate; novel; programs; repository; sample collection; success; tool; virology

The Laboratory Sciences Core, Core D, under the direction of Dr Savita Pahwa (Director), Dr Rafick Sekaly (Co-Director), Dr. Geoffrey Stone (Core Coordinator) and recently recruited Co-Director of the CFAR, Dr Mario Stevenson will provide state-of-the-art assays in immunology, macrophage biology and molecular virology as well as expertise in systems biology. In the transition to a full CFAR, the Core has broadened its services to include systems biology and special virology. Core D laboratories that will serve the CFAR consist of I), the Immunology laboratory which performs specialized immunology assays and processes samples for the Miami CFAR biorepository; II), Sub-core 1, the Systems Biology Laboratory (new) at the Vaccine and Gene Therapy Institute (VGTI) in Port St. Lucie will provide consultation and bioinformatics support for gene array and related assays; HI), Sub-Core 2, the Special Virology Laboratory (new) that will offer molecular virology and purified myeloid-lineage cells by elutriation and IV, the CLIA approved Diagnostic Laboratory that performs routine CD4 and virus load assays and is also a back-up for cryopreservation of repository samples. The specific Aims of Core D are: 1. To provide access to specialized, innovative and standardized immunologic assays for HIV/SIV immunopathogenesis and vaccine research, by providing immunologic services such as multiparameter flow cytometry, T- and B cell specific ELISPOT, assessment of cytokines, microbial translocation, thymus function, and immune monitoring protocols for investigations in human and non-human primate cells. The Core provides purified cell populations as well as protocols for manipulation (transfection, RNAi) of primary cells and links up investigators with specialized laboratories. 2. To provide capacity-building consultation for genomic assays and bioinformatic analysis performed at Vaccine and Gene Therapy Institute (VGTI) at Port St. Lucie, FL; 3. To provide special virology and elutriated monocytes to CFAR investigators in the special virology sub-core. 4. To promote educational activities and to provide consultation/training for new CFAR-supported developmental grant awardees in collaboration with the Developmental Core, Core B and to T32 Trainees, 5. To assist in the CFAR objectives of developing scientific areas of research (SAR), by provision of laboratory resources to SAR members, and 6. To assist in development of repositories of clinical cohorts including HIV-negative and HIV-positive cohorts in collaboration with Clinical Sciences Core (Core C) and Behavioral and Social Sciences and Community Outreach Core (Core E).

实验室科学核心D，在Savita Pahwa博士（主任），Rafick Sekaly博士（联合主任），Geoffrey Stone博士（核心协调员）和最近招募的CFAR联合导演Mario Stevenson博士将提供免疫学生物学和摩洛噬学生物学领域的现状分析。在过渡到完整的CFAR时，核心扩大了其服务，包括系统生物学和特殊病毒学。将为CFAR提供服务的核心D实验室I）是I）的免疫学实验室，该实验室对迈阿密CFAR Biorepository进行了专门的免疫学分析和处理样品； ii），第1个，疫苗和基因治疗研究所（VGTI）的系统生物学实验室（NEW）将为基因阵列和相关测定法提供咨询和生物信息学支持； HI），亚核2，特殊的病毒学实验室（NEW），它将通过Elutriation和IV提供分子病毒学并纯化的髓样细胞和IV，CLIA批准进行常规CD4和病毒负荷测定法，也是用于冷冻保存repository样品的备份。 The specific Aims of Core D are: 1. To provide access to specialized, innovative and standardized immunologic assays for HIV/SIV immunopathogenesis and vaccine research, by providing immunologic services such as multiparameter flow cytometry, T- and B cell specific ELISPOT, assessment of cytokines, microbial translocation, thymus function, and immune monitoring protocols for investigations in human and non-human primate细胞。该核心提供了纯化的细胞群以及原代细胞操纵（转染，RNAi）的方案，并将研究人员与专门的实验室联系起来。 2。为在佛罗里达州圣卢西港（VGTI）进行的基因组测定和生物信息学分析提供能力建设咨询； 3。为特殊病毒学子核的CFAR研究者提供特殊的病毒学和精髓单核细胞。 4。促进教育活动并为新的CFAR支持的发展赠款获奖者提供咨询/培训，以与发展核心，核心B和T32受训者合作，为5。，共同协助开发科学领域的CFAR目标（SAR），通过向SAR成员提供SAR成员以及6次临床COSILITION HIV-HIV-IV-INV-INV-INV-INV-INFIV-INF-INV-INV-IND-INF-INV-IND-IN。具有临床科学核心（核心C）以及行为和社会科学以及社区外展核心（核心E）。