CHARACTERIZATION OF THE DSIK3 PROTEIN

DSIK3 蛋白的表征

• 批准号: 8171465
• 负责人: MARC R MONTMINY
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171465
• 关键词: Burn injury; Computer Retrieval of Information on Scientific Projects Database; Fat Body; Fatty acid glycerol esters; Funding; Glucose; Grant; Institution; Mass Spectrum Analysis; Obesity; Pathway interactions; Phosphorylation; Process; Proteins; Research; Research Personnel; Resources; Source; Transgenic Organisms; United States National Institutes of Health; energy balance; feeding; fly; human disease; salt-inducible kinase

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In this study we performed mass spectrometry studies on SIK3, salt inducible kinase, immunoprecipitates from transgenic flies to identify residues in SIK3 that undergo phosphorylation in a fat body during feeding. This study is important as metazoans maintain energy balance by shifting from glucose to fat burning and understanding the pathways that regulate this process will further our understanding of human disease such as obesity.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 在这项研究中，我们对转基因果蝇的 SIK3（盐诱导激酶）和免疫沉淀物进行了质谱研究，以鉴定 SIK3 中在进食过程中在脂肪体内经历磷酸化的残基。 这项研究很重要，因为后生动物通过从葡萄糖转变为脂肪燃烧来维持能量平衡，了解调节这一过程的途径将进一步我们对肥胖等人类疾病的理解。