POSTTRANSLATIONAL MODIFICATIONS ON HISTONES AND HISTONE VARIANTS

组蛋白和组蛋白变异体的翻译后修饰

• 批准号: 8169757
• 负责人: BARBARA PANNING
• 金额: $ 0.35万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-12 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169757
• 关键词: Acetylation; Cells; Chromatin; Chromatin Structure; Computer Retrieval of Information on Scientific Projects Database; Development; Epigenetic Process; Funding; Gene Expression; Genetic; Grant; Histones; Institution; Lead; Maintenance; Malignant Neoplasms; Memory; Methylation; Modification; Nature; Organism; Pattern; Phosphorylation; Post-Translational Protein Processing; Research; Research Personnel; Resources; Role; Source; Specificity; Ubiquitination; United States National Institutes of Health; Variant; cell type; combinatorial; flexibility; programs; tandem mass spectrometry

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Multicellular organisms consist of various cell types with the same genetic information but a high degree of differentiation, characterized by a unique pattern of gene expression for each cell type. Establishment and maintenance of these diverse expression patterns is fundamentally important for cell identity and organism survival, and aberrant gene expression in a single cell can lead to developmental abnormalities or cancer. Epigenetic regulatory mechanisms employ dynamic modifications in chromatin structure, such as histone methylation, acetylation, phosphorylation and ubiquitination, to regulate gene expression. These posttranslational modifications are integrated in a combinatorial fashion to provide cells with transcriptional memory to stably maintain gene expression patterns throughout many divisions, and developmental flexibility to facilitate programmed alterations in gene expression. Equipped with the unprecedented sensitivity and structural specificity offered by tandem mass spectrometry, we are hoping to elucidate the nature and functional role of the rich epigenetic information in mammalian chromatin.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 多细胞生物由具有相同遗传信息但高度分化的各种细胞类型组成，其特征是每种细胞类型具有独特的基因表达模式。 这些不同表达模式的建立和维持对于细胞身份和生物体生存至关重要，单细胞中的异常基因表达可能导致发育异常或癌症。 表观遗传调控机制利用染色质结构的动态修饰（例如组蛋白甲基化、乙酰化、磷酸化和泛素化）来调节基因表达。 这些翻译后修饰以组合方式整合，为细胞提供转录记忆，以在多次分裂中稳定维持基因表达模式，并提供发育灵活性，以促进基因表达的程序性改变。 凭借串联质谱提供的前所未有的灵敏度和结构特异性，我们希望阐明哺乳动物染色质中丰富的表观遗传信息的性质和功能作用。