CHARACTERIZATION OF SATB1 MODIFICATIONS FOLLOWING IONIZATION RADIATION

电离辐射后 SATB1 修饰的表征

• 批准号: 8170703
• 负责人: Terumi Kohwi-Shigematsu
• 金额: $ 3.21万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170703
• 关键词: Adult; Affinity; Binding Proteins; Computer Retrieval of Information on Scientific Projects Database; DNA; Funding; Genes; Goals; Grant; Histone Code; Institution; Laboratories; Modification; Nuclear; Post-Translational Protein Processing; Proteins; Radiation; Regulator Genes; Research; Research Personnel; Resources; Signal Transduction; Source; Staging; Stem cells; Testing; United States National Institutes of Health; adult stem cell; base; cell type; embryonic stem cell; ionization; irradiation; response; thymocyte

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction and Rationale A major goal of my laboratory has been to understand the fundamental mechanisms by which a large body of genes is directed to be either expressed or repressed in a cell-type or stage-specific manner or in response to signals from outside. In this context, we identified a nuclear architectural protein, SATB1, which functions as a global gene regulator, expressed in embryonic stem (ES) cells and adult progenitor cells, such as thymocytes. Using a DNA affinity column containing BUR (base unpairing region) specifically recognized by SATB1, we wish to identify SATB1 associated proteins as well as new BUR-binding proteins in stem cells and adult progenitor cells. Furthermore, we wish to test a hypothesis that the SATB1 protein undergoes changes in post-translational modification during differentiation or upon irradiation. The goal of the project is to assign specific functions to each type of post-translational modification of the SATB1 protein, similar to the concept of the 'histone code'.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 简介和基本原理我的实验室的一个主要目标是了解大量基因以细胞类型或特定阶段的方式或响应外部信号而表达或抑制的基本机制。 在这种情况下，我们鉴定了一种核结构蛋白 SATB1，它作为全局基因调节因子，在胚胎干 (ES) 细胞和成体祖细胞（如胸腺细胞）中表达。 使用含有 SATB1 特异性识别的 BUR（碱基不配对区域）的 DNA 亲和柱，我们希望鉴定 SATB1 相关蛋白以及干细胞和成体祖细胞中新的 BUR 结合蛋白。 此外，我们希望检验一个假设，即 SATB1 蛋白在分化过程中或照射后会发生翻译后修饰的变化。该项目的目标是为 SATB1 蛋白的每种类型的翻译后修饰分配特定的功能，类似于“组蛋白密码”的概念。