Animal Shared Resource

动物共享资源

• 批准号: 8180849
• 负责人: Louis M. Weiner
• 金额: $ 6.86万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-06 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8180849
• 关键词: Animal; Shared; Resource

The function of Animal Shared Resource (ASR) is to facilitate the efficient, economical, and state-of-art use of animal models for the performance of cancer-related studies. This is accomplished through a centralized resource, where AALAS-certified veterinary technicians/technologists provide high-quality research services to Lombardi members. A significant emphasis is placed on the use of genetically modified mice, athymic mice, and zebrafish. Major services provided include coordination of animal and special animal feed purchase requests; performance of specific manipulations, including diet, hormone or drug administrations to modify tumor inflation, promotion, progression or metastasis; inoculation of tumor cells or in vivo propagation of tumor cell lines in animals; administration of carcinogens; monitoring tumor growth; collection of tumors, organs, and sera; performance of necropsies and biopsies; and zebrafish studies, including microinjecting eggs with antisense Morpholino oligonucleotides (MO) to knock down specific genes of interest; microinjecting eggs with RNA or cDNA to overexpress wild-type, mutant and chimeric genes; screening for toxicity/drugs; generating transgenic zebrafish; transplanting cells; and xenotransplantation experiments. The ASR is located within Georgetown University Medical School's Division of Comparative Medicine (DCM). The DCM is a centralized, AAALAC-accredited, USDA-registered animal facility and has an approved letter of assurance on file at the NIH. The general environment and animal health is monitored through the use of sentinel mice maintained on each rack in each animal room as part of a comprehensive health surveillance program. All immune-deficient rodents are maintained within a Specific Pathogen Free environment. Most genetically modified mice colonies are maintained in a dedicated barrier isolation facility. The services of this resource are critical for the Lombardi investigators who perform preclinical studies to test hypotheses related to treatment and prevention of cancer using state-of-art, and appropriate, effective and economical animal models. The highly trained and experienced technical staff works closely with Lombardi members to provide quality animal model technical services in support of peer reviewed cancer research requiring the use of animals. In 2008, these services were used in support of peer-reviewed projects of 18 Lombardi members in five Cancer Center Programs.

动物共享资源 (ASR) 的功能是促进高效、经济且最先进地使用动物模型来进行癌症相关研究。这是通过集中资源实现的，其中 AALAS 认证的兽医技术人员/技术专家为 Lombardi 成员提供高质量的研究服务。重点强调使用转基因小鼠、无胸腺小鼠、 和斑马鱼。提供的主要服务包括协调动物和特殊动物饲料的采购请求；执行特定操作，包括饮食、激素或药物管理以改变肿瘤膨胀、促进、进展或转移；肿瘤细胞的接种或肿瘤细胞系在动物体内的繁殖；致癌物质的管理；监测肿瘤生长；收集肿瘤、器官和血清；进行尸检和活组织检查；斑马鱼研究，包括向卵子显微注射反义吗啡寡核苷酸 (MO) 以敲除感兴趣的特定基因；将 RNA 或 cDNA 显微注射到卵子中，以过度表达野生型、突变型和嵌合型基因；毒性/药物筛查；产生转基因斑马鱼；移植细胞；和异种移植实验。 ASR 位于乔治城大学医学院的比较医学部 (DCM)。 DCM 是一家经 AAALAC 认证、美国农业部注册的集中动物设施，并在 NIH 备案有一份批准的保证书。作为综合健康监测计划的一部分，通过使用每个动物房的每个架子上饲养的哨兵小鼠来监测总体环境和动物健康。都是免疫力低下的 啮齿动物饲养在无特定病原体的环境中。大多数转基因小鼠群体都保存在专用的屏障隔离设施中。该资源的服务对于 Lombardi 研究人员至关重要，他们使用最先进、适当、有效和经济的动物模型进行临床前研究，测试与癌症治疗和预防相关的假设。训练有素且经验丰富的技术人员与 Lombardi 成员密切合作，提供优质的动物模型技术服务，以支持需要使用动物的同行评审癌症研究。 2008 年，这些服务被用于支持 5 个癌症中心项目的 18 名 Lombardi 成员的同行评审项目。