Acquisition of Shared Thermoneutral Rodent Housing Resources

获取共享的热中性啮齿动物住房资源

• 批准号: 10734172
• 负责人: Alan Daugherty
• 金额: $ 18.21万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-15 至 2024-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10734172
• 关键词: Address; Affect; Animal Experimentation; Animal Housing; Animals; Area; Awareness; Basal metabolic rate; Behavioral; Biomedical Research; Blood Pressure; Breeding; Calibration; Cardiac; Chronic; Complement; Core Facility; Data; Dedications; Disease; Disease Outcome; Eating; Energy Metabolism; Environmental Impact; Equipment; Faculty; Floor; Funding; Goals; Health; Heart Rate; House mice; Housing; Human; Immune response; Individual; Intervention; Kentucky; Laboratories; Laboratory Animals; Laboratory mice; Literature; Maintenance; Metabolic Diseases; Modeling; Modernization; Mus; Nerve Degeneration; Outcome Study; Outcomes Research; Pathologic; Pathology; Physiological; Plants; Procedures; Process; Productivity; Publications; Research; Research Activity; Research Design; Research Personnel; Research Support; Resistance; Resources; Rodent; Services; Skeletal Muscle; Sleep; Stress; System; Temperature; Translations; United States National Institutes of Health; Universities; Vascular Dementia; animal facility; animal resource; behavioral study; circadian; cold stress; cost; design; experimental study; fetal programming; genetic manipulation; human disease; immune function; muscle hypertrophy; operation; research facility; response; square foot; tumor growth; ventilation

PROJECT SUMARY/ABSTRACT The need to house and maintain laboratory rodents under conditions of thermoneutrality, i.e., at the ambient temperature at which energy expenditure is limited to that required to maintain basal metabolic rate, is increasingly recognized as critical to achieving rigorous research outcomes. Evidence of temperature effects on a vast array of physiological functions and pathologies spanning energy expenditure, immune function and response, blood pressure, heart rate, sleep, food intake, tumor growth and resistance, and more have raised awareness of the need to factor thermoneutrality into research designs to enhance the validity of research findings and their translation to understand and enhance human health. To accommodate recent and ongoing campus expansion of research capabilities for conducting biomedical studies in mice under thermoneutrality, the University of Kentucky (UK) proposes to establish a Thermoneutrality Suite in its newest vivarium in the recently constructed Healthy Kentucky Research Building (HKRB). The 3,000 sq. ft. HKRB vivarium supports a 265,000 sq. ft. six-floor state-of-the-art biomedical research facility that opened in 2018 and is contiguous to animal facilities in two adjacent modern research buildings. The long-term goal of this equipment application is to enhance and modernize animal research support operations managed by the UK Division of Laboratory Animal Resources (DLAR) by equipping a three-room Thermoneutrality Suite (970 sq. ft. of space) with dedicated advanced thermoneutrality caging. The proposed suite, to be operated by DLAR and overseen by the Associate Vice President for Research Core Facilities, will achieve the specific aim to permit both the breeding and housing of mouse colonies at thermoneutrality by acquiring six mobile Solace Zone IVC caging systems to be installed in a 385 sq. ft. space adjoining two rooms equipped for experimentation under thermoneutral study conditions. This request to the NIH will complement acquisition of separately acquired thermoneutral experimental equipment to be consolidated in two adjacent rooms, but not part of this funding request. The completed suite will elevate DLAR’s research-supporting operations for NIH-funded investigators who studies require access to three Power Scientific Inc. model RIS70SD rodent experimental chambers to support behavioral studies and four Actimetrics Clocklab Chambers suitable for performing circadian studies, one of which is already in place. The HKRB vivarium is a modern, high-end facility. This facilities upgrade will enable new research efficiencies and provide enhanced rigor to researchers whose study objectives require carefully calibrated environmental conditions through an integrated research support operation. Addressing this need for cutting-edge mobile animal housing units will impact diverse NIH-funded studies spanning cardiac arrythmias, vascular dementia and neurodegeneration, skeletal muscle hypertrophy, metabolic disease, fetal programming, and more to produce more rigorous study outcomes. These areas represent some of UK’s most prominent areas of research activity conducted by a highly productive and collaborative faculty.

项目总结/摘要 需要在热中性条件下（即环境温度）饲养和维持实验室啮齿动物 能量消耗仅限于维持基础代谢率所需的温度为 越来越多的人认识到温度影响的证据对于取得严格的研究成果至关重要。 涉及广泛的生理功能和病理学，涵盖能量消耗、免疫功能和 反应、血压、心率、睡眠、食物摄入、肿瘤生长和抵抗力等都有所提高 意识到需要将热中性纳入研究设计以提高研究的有效性 研究结果及其转化以了解和增强人类健康以适应最近和正在进行的情况。 校园扩展研究能力，在热中性条件下对小鼠进行生物医学研究， 肯塔基大学（英国）提议在其最新的动物园中建立一个热中性套房 最近建成的健康肯塔基研究大楼 (HKRB) 占地 3,000 平方英尺的 HKRB 动物园支持一个 占地 265,000 平方英尺的六层最先进的生物医学研究设施于 2018 年开业，毗邻 两个相邻的现代研究大楼的动物设施该设备应用的长期目标是。 加强英国实验室部门管理的动物研究支持业务并使之现代化 动物资源 (DLAR)，为三室热中性套房（970 平方英尺的空间）配备 拟议的专用高级热中性笼舍由 DLAR 运营并由其监督。 负责研究核心设施的副总裁将实现具体目标，以允许 通过购买六个移动 Solace Zone IVC 笼子，在热中性条件下繁殖和饲养小鼠群体 系统将安装在 385 平方英尺的空间内，毗邻两个配备实验的房间 向 NIH 提出的这一请求将补充单独获得的研究条件。 热中性实验设备将合并在两个相邻的房间，但不属于本次资助的一部分 完成的套件将提升 DLAR 对 NIH 资助的研究人员的研究支持业务。 谁的研究需要使用三个 Power Scientific Inc. 型号 RIS70SD 啮齿动物实验室 支持行为研究和四个适合进行昼夜节律研究的 Actimetrics Clocklab Chambers， 其中一个已经到位，HKRB 动物园是一处现代化的高端设施，此次设施升级将进行。 实现新的研究效率，并为研究目标需要的研究人员提供增强的研究 通过综合研究支持行动仔细校准环境条件来解决这个问题。 对尖端移动动物饲养单元的需求将影响 NIH 资助的各种心脏研究 心律失常、血管性痴呆和神经变性、骨骼肌肥大、代谢性疾病、胎儿 编程等，以产生更严格的研究成果，这些领域代表了英国最重要的一些领域。 由高产和协作的教师进行的突出研究活动领域。