BEHAVIORAL CORE

行为核心

基本信息

批准号：
8214168
负责人：
Mehrdad Shamloo
金额：
$ 24.13万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8214168
关键词：
Advisory Committees Animal Experimentation Animal Model Animals Behavior Behavior assessment Behavioral Behavioral Model Biological Assay Biological Process Collaborations Communities Computer software Consultations Development Disease Environment Evaluation Experimental Designs Faculty Generations Genes Genetic Goals Home environment Housing Human IACUC Individual Interruption Investigation Knockout Mice Laboratories Microscopy Modeling Molecular Monitor Mus Mutant Strains Mice Neuraxis Neurosciences Neurosciences Research Outcome Phenotype Principal Investigator Process Protocols documentation Rattus Reporting Reproducibility Research Design Research Personnel Resources Rodent Services Sleep Wake Cycle Solutions Stress Supervision System Testing Transgenic Mice Transportation Universities Variant Work Writing base behavior test behavioral pharmacology data acquisition design experience genetic manipulation in vivo mouse model new technology novel preclinical study research study social group sound vector viral gene delivery

项目摘要

In recent years there has been an increase in the generation of transgenic and knockout mouse lines as well as viral delivery of genes. These new genetic manipulations have revolutionized our understanding of the biological function of cellular and molecular processes in both normal and disease state of central nervous system ^^¿^^. The large numberof mouse models calls for rigorous, sensitive, and reproducible investigations of behavioral phenotypes of mutant mice lines ^^ ^¿, as well as pharmacological efficacy studies. Behavioral testing paradigms should be easily transferable and replicable between laboratories. Many of the standard behavioral tests used today are not easily reproduced between laboratories, due either to variation in the genetic background of mice ^^' ^¿, or to variations in environmental or experimental conditions ^^' ^^. Other confounding factors can include variations in animal handling, housing, transportation, and test conditions ^^¿^^¿ ^. Behavioral models used for phenotyping of mice are designed based on the models that have been designed for rats as the test subjects ^^. Often these classical tests do not satisfactorily transfer over to mice. Additionally, these standard tests introduce many outside variables such as the effects of environment and human handling ^\ forced isolation, interruption of sleep/wake cycle, and placement in unnatural, stressful environments. In order to minimize these experimental and environmental variables, we will establish an Automated Behavioral Core to monitor and record mouse behavior in the home cage environment. These novel tests have the benefit of decreasing variability and increasing reproducibility of behavioral outcome between experimental repeats ^^"^¿. By using these new technologies we are aiming to 1) reduce novelty factors inherent in many classical tests by performing the behavioral testing inside the home cage ^^; 2) test the Individual animals while they remain in social groups (Intellicage) or when they are individually housed (PhenoLab); 3) reduce the stress related to the handling and experimental condition; 4) perform the data acquisition using computer software in an independent and unbiased manner; and 5) conduct the experiments with higher throughput during the animals active cycle without human interruption.

近年来，转基因和基因敲除小鼠品系的产生以及基因的病毒传递不断增加，这些新的基因操作彻底改变了我们对中枢正常和疾病状态下细胞和分子过程的生物学功能的理解。紧张的系统^^¿ ^^. 大量的小鼠模型需要对突变小鼠系的行为表型进行严格、灵敏且可重复的研究^^ ^¿以及药理学功效研究应该可以在实验室之间轻松转移和复制。由于小鼠遗传背景的差异，当今使用的行为测试在实验室之间不容易重现 ^^' ^¿ ，或环境或实验条件的变化 ^^' ^^ 其他混杂因素可能包括动物处理、饲养、运输和测试条件的变化 ^^¿ ^^¿ ^. 用于小鼠表型分析的行为模型是根据为作为测试对象的大鼠设计的模型而设计的 ^^ 通常这些经典测试不能令人满意地转移到小鼠身上。此外，这些标准测试引入了许多外部变量，例如环境和人类处理的影响、强制隔离、睡眠/觉醒周期中断以及放置在不自然、有压力的环境中。为了最大限度地减少这些实验和环境变量，我们将。建立一个自动化行为核心用于监测和记录家笼环境中的小鼠行为，这些新颖的测试具有减少实验重复之间行为结果的变异性和提高重复性的优点^^"^¿。通过使用这些新技术，我们的目标是 1)通过在笼子内进行行为测试来减少许多经典测试中固有的新颖性因素 ^^ 2) 在个体动物处于社会群体中时（Intellicage）或单独饲养时进行测试（PhenoLab）3）；减少与操作和实验条件相关的压力；4）以独立且公正的方式使用计算机软件进行数据采集，5）进行实验；在动物活动周期中具有更高的吞吐量，无需人为干扰。