Eye Movement Maps in Mouse Superior Colliculus

小鼠上丘眼动图

• 批准号: 8594491
• 负责人: Jianhua Cang
• 金额: $ 23.18万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8594491
• 关键词: Animals; Anterior; Biological Models; Birth; Clinical; Data; Development; Disease; Epilepsy; Eye Movements; Felis catus; Foundations; Future; Genetic; Goals; Head; Human; Individual; Investigation; Location; Maps; Mental Retardation; Motor; Motor output; Movement; Mus; Nervous system structure; Neurologic; Neurons; Ocular Motility Disorders; Penetration; Preparation; Primates; Research; Research Personnel; Retinal; Role; Saccades; Schizophrenia; Sensory; Signal Transduction; Site; Structure; Transgenic Mice; Visual; Visual system structure; Wild Type Mouse; autism spectrum disorder; awake; electrical microstimulation; experience; islet; motor control; public health relevance; rapid eye movement; receptive field; relating to nervous system; research study; retinotopic; sensorimotor system; superior colliculus Corpora quadrigemina; tool; vision development; visual map; visual motor; visual receptive field

Project Summary/Abstract The mammalian superior colliculus (SC) is a subcortical structure that integrates visual and other sensory information to initiate orienting movements of the eyes and head. A fundamental feature of SC organization is that the representations of sensory inputs and motor outputs are topographically arranged and aligned. While great progress has been made in understanding the development of the visual representation in the SC, how the motor maps are formed and aligned with the visual map remains unknown. In order to take advantage of the available genetic tools in mice, the investigators propose to perform a comprehensive investigation of the organization and development of motor maps in the mouse SC. First, electrical microstimulation will be conducted in the deep layers of the SC to evoke saccade-like rapid eye movements in mice. The topographic organization of the eye movement map will be revealed by systematically varying the stimulation sites in the SC and determining the amplitude and direction of the evoked movements. Single-unit recording will also be performed in the deep layers to determine how individual neurons encode eye movement direction and amplitude, and how such movement fields are mapped in the mouse SC. Second, the same experiments will be performed in mice deprived of visual experience from birth and in an existing transgenic mouse line that have altered visual maps in the SC. These experiments will determine whether visual inputs provide an instructive signal for motor map development and visuomotor alignment. Together, the proposed experiments will provide the first systematic mapping of the deep layers of the mouse SC and initiate studies on factors influencing sensorimotor development. These studies will have important implications for the understanding and potential treatment of human eye movement disorders and other diseases that result from miswiring of neuronal connections. 6

项目摘要/摘要 哺乳动物上丘（SC）是一种皮层结构，可整合视觉和其他 感官信息以启动眼睛和头部的方向运动。 SC的基本特征 组织是感官输入和电动机输出的表示形式是地形安排的， 对齐。尽管在理解视觉表示的发展方面取得了巨大进展 SC，如何形成电动图并与视觉图对齐仍然未知。为了接受 小鼠可用遗传工具的优势，调查人员建议执行全面的 对小鼠SC中电机图的组织和发展的调查。首先，电气 微刺激将在SC的深层中进行，以引起扫视的快速眼动。 老鼠。眼动图的地形组织将通过系统地改变 SC中的刺激位点并确定诱发运动的幅度和方向。单单元 还将在深层进行记录，以确定单个神经元如何编码眼睛运动 方向和振幅，以及如何在小鼠SC中映射此类运动场。第二，相同 实验将在从出生和现有转基因中剥夺视觉体验的小鼠中进行 鼠标线改变了SC中的视觉图。这些实验将确定视觉输入是否 为电机图的开发和视觉电机比对提供有指导的信号。一起，提议 实验将提供小鼠SC深层的第一个系统映射并启动研究 关于影响感觉运动发展的因素。这些研究将对 了解和潜在治疗人类眼动障碍和其他疾病 神经元连接的关闭误差。 6