TRANSGENIC MONKEY MODEL OF HUNTINGTON?S DISEASE - SUPPLEMENT

亨廷顿病转基因猴模型 - 补充材料

• 批准号: 8357569
• 负责人: ANTHONY WING SANG CHAN
• 金额: $ 3.29万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357569
• 关键词: Age-Months; Behavior; Biological; Biological Markers; Blood; Blood specimen; Budgets; Cognitive; Collection; Disease; Evaluation; Funding; Gene Expression Profiling; Genome; Grant; Huntington Disease; Image; Longitudinal Studies; MicroRNAs; Modeling; Molecular Profiling; Monitor; Monkeys; National Center for Research Resources; Pattern; Primates; Principal Investigator; Research; Research Infrastructure; Resources; Source; Tissue-Specific Gene Expression; Transgenic Organisms; United States National Institutes of Health; cost; parent grant; peripheral blood

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In this supplemental study, we will monitor the gene expression and miRNA expression profile of the peripheral blood of these HD monkeys at six months intervals throughout the two budgeted years. While peripheral blood samples have already been collected from some of the HD and WT-monkeys at six and twelve month of age, we propose that continuing blood collection occur at 18, 24, 30 and 36 month of age as described in the proposed study. Once the platform of the genome and miRNA profiling studies is established, continued monitoring of peripheral blood will be performed parallel to the other original HD monkeys' longitudinal studies supported by the parent grant, which include cognitive, behavior evaluation, and noninvasive imaging. We hypothesize that analysis of peripheral blood could reveal unique differential gene expression patterns and unique expression profiles of miRNA, which could be used as an indicator of abnormal biological conditions in HD, thus biomarkers of HD progression could be identified.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 在这项补充研究中，我们将在两个预算年度中每隔六个月监测这些 HD 猴子外周血的基因表达和 miRNA 表达谱。虽然已经从一些 6 月龄和 12 月龄的 HD 和 WT 猴身上采集了外周血样本，但我们建议按照拟议研究中的描述，在 18、24、30 和 36 月龄时继续进行血液采集。一旦基因组和 miRNA 分析研究平台建立，外周血的持续监测将与父母资助支持的其他原始 HD 猴子的纵向研究并行进行，其中包括认知、行为评估和无创成像。我们假设外周血分析可以揭示独特的差异基因表达模式和独特的 miRNA 表达谱，这可以用作 HD 异常生物学状况的指标，从而可以识别 HD 进展的生物标志物。