cell Function & Pathophysiology Project

细胞功能

• 批准号: 8551297
• 负责人: Ted M. Dawson
• 金额: $ 16.89万
• 依托单位: MCLEAN HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8551297
• 关键词: Affect; AlamarBlue; Amphetamines; Apomorphine; Axon; Biogenesis; Biological; Biological Assay; Calcium; Cell Line; Cell physiology; Cells; Cellular Morphology; Clinical Investigator; Complement; Confocal Microscopy; Cytosol; Data; Dendrites; Disease; Dopamine; Dose; Dyes; Environment; Enzyme-Linked Immunosorbent Assay; Evaluation; Free Radicals; Functional disorder; Gene Mutation; Gene Targeting; Generations; Genes; Genotype; Genus Hippocampus; Glucose; Human; Image; Immunohistochemistry; In Vitro; Individual; Investigation; Ion Channel; Ions; LRRK2 gene; Laboratories; Lewy Bodies; Life; Link; Measurement; Measures; Membrane Potentials; Mitochondria; Mitochondrial Matrix; Modeling; Monitor; Movement; Mus; Mutation; Neurites; Neurons; Other Genetics; Oxidation-Reduction; PINK1 gene; Pathogenesis; Pathology; Pathway interactions; Phenotype; Phosphorylation; Physiological; Play; Population; Preclinical Drug Evaluation; Process; Proteins; Rattus; Reactive Oxygen Species; Recruitment Activity; Reporter; Research; Respiration; Reverse Transcriptase Polymerase Chain Reaction; Role; Scientist; Seeds; Shapes; Side; Staining method; Stains; Structure; Techniques; Toxin; Transmission Electron Microscopy; Transplantation; Western Blotting; Work; adeno-associated viral vector; aged; alpha synuclein; base; cellular imaging; cytotoxicity; dopaminergic neuron; glucose metabolism; immunocytochemistry; in vivo; in vivo Bioassay; induced pluripotent stem cell; meetings; member; mitochondrial membrane; neuronal cell body; novel strategies; parkin gene/protein; patch clamp; receptor; relating to nervous system; screening; synaptogenesis

The Cell Function and Pathophysiology Core will be led by Dr. Ted Dawson and will function to determine disease-associated phenotypes from the differentiated PDiPS cell lines. In addition, the members of the Cell Function and Pathophysiology Core will be responsible for developing the existing phenotypic assays into a format amenable for large-scale drug screening. In conclusion, we think that the talented clinical investigators and translational scientists that comprise our current PDiPS Consortium are well placed to transition our groundbreaking work into a U24 format that meets the criteria outlined in the new RFA (RFA-NS-11-011, see Fig. 3 for Milestones).

细胞功能和病理生理核心将由Ted Dawson博士领导，将 从分化的PDIPS细胞系确定与疾病相关的表型的功能。此外，细胞功能和病理生理学核心的成员将负责将现有的表型测定开发为适合大规模药物筛查的格式。总之，我们认为，构成我们当前PDIPS联盟的才华横溢的临床研究人员和翻译科学家都可以很好地适合 将我们的开创性工作转变为U24格式，该格式符合新RFA中概述的标准（RFA-NS-11-11-011，有关里程碑，请参见图3）。