Non-contact, THz Sensing of Corneal Hydration

非接触式太赫兹角膜水合感测

• 批准号: 8889681
• 负责人: WARREN S GRUNDFEST
• 金额: $ 30.74万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8889681
• 关键词: Ablation; Animals; Calibration; Characteristics; Cornea; Corneal Diseases; Data; Diagnosis; Disease; Ensure; Eye; Fuchs' Endothelial Dystrophy; Goals; Histology; Human; Hydration status; Image; Imaging Device; Institutional Review Boards; Laser In Situ Keratomileusis; Lighting; Maps; Measurement; Measures; Methods; Modeling; Monitor; Operative Surgical Procedures; Oryctolagus cuniculus; Patients; Photons; Proteomics; Radiation; Resolution; Safety; Spectrum Analysis; System; Techniques; Technology; Testing; Thick; Ultrasonography; Water; eye center; imaging modality; imaging system; improved; in vivo; mathematical model; new technology; operation; phantom model; phenomenological models; simulation; tool development; volunteer

DESCRIPTION (provided by applicant): The goal of this proposal is to develop a THz imaging system to image and spatially map the hydration of the cornea. Corneal hydration is currently measured using ultrasound pachymetry and is derived from an empiric relationship between central corneal thickness and available hydration data. This proposal will use corneal spectroscopy and reflectance data to improve models of THz reflectivity from the cornea. These models will be evaluated using phantoms and in-vivo rabbit corneas. Preliminary data suggest that THz imaging can measure corneal hydration with 0.1% accuracy and a spatial resolution of 600 um. The simulations and phantom models, and animal studies will be used to evaluate the feasibility of the approach and verify the ability of corneal THz imaging to discern hydration changes characteristic of corneal diseases. One of the advantages of THz radiation is its extremely low photon energy. It is eye safe and operates at 10 - 20 uW. To ensure safety for human use we will evaluate THz illumination on the cornea of rabbits at 50 5W and examine the corneas with histology and proteomic analysis. Once we have confirmed safety and obtained necessary IRB approvals we will conduct initial human corneal imaging trials in normal volunteers. After obtaining baseline information from normal patients (N = 60) we will proceed to study hydration in patients before and after DSEK surgery (N = 20), and before and after LASIK surgery (N = 20). Data accumulated from these studies will be analyzed to assess the feasibility of THz imaging to monitor hydration levels as a function of disease state. 1

描述（由申请人提供）：该提案的目的是开发一个THZ成像系统，以形象和空间绘制角膜的水合。目前，使用超声波处理来测量角膜水合，并源自中央角膜厚度与可用水合数据之间的经验关系。该建议将使用角膜光谱和反射率数据来改善角膜的THZ反射率模型。这些模型将使用幻像和体内兔角膜进行评估。初步数据表明，THZ成像可以以0.1％的精度测量角膜水合，空间分辨率为600 um。模拟和幻影模型以及动物研究将用于评估方法的可行性，并验证角膜THZ成像识别水合变化角膜疾病的特征的能力。 THZ辐射的优点之一是其极低的光子能量。它是安全的，可在10-20 UW运行。为了确保人类使用的安全性，我们将评估50 5W兔子角膜的Thz照明，并通过组织学和蛋白质组学分析检查角膜。一旦我们确认了安全性并获得了必要的IRB批准，我们将在正常志愿者中进行初始的人角膜成像试验。从正常患者那里获得基线信息（n = 60）后，我们将继续研究DSEK手术前后的患者（n = 20）以及Lasik手术前后的水合（n = 20）。将分析从这些研究中积累的数据，以评估THZ成像的可行性，以监测水合水平与疾病状态的关系。 1