THz imaging of acute burn wounds

急性烧伤创面太赫兹成像

• 批准号: 8512614
• 负责人: WARREN S GRUNDFEST
• 金额: $ 21.2万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-04-10 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8512614
• 关键词: Acute; Animals; Area; Blood; Burn Centers; Burn injury; Calibration; Clinical; Coagulation Process; Companions; Data; Diagnosis; Dyes; Edema; Eye; Feedback; Goals; Hematoxylin and Eosin Staining Method; Hour; Hydration status; Image; Imagery; Imaging Device; Injury; Lasers; Lighting; Literature; Maps; Measurement; Measures; Methods; Modeling; Pathologic; Pathology; Photons; Rattus; Resolution; Scanning; Secondary to; Severities; Staining method; Stains; Surgeon; System; Tactile; Techniques; Thick; Tissues; Visual; Water; Work; absorption; base; blood perfusion; diagnostic accuracy; experience; heat injury; in vivo; prevent; public health relevance; research study; response; tool; wound

DESCRIPTION (provided by applicant): The goal of this proposal is to develop a tissue hydration imaging system based on THz and NIR illumination to spatially and temporally map the hydration state of burn wounds and correlate these measurements with burn severity. Burn severity is typically assessed with visual and tactile feedback from the surgeon and diagnostic accuracy varies widely based on the clinical presentation and the surgeon's experience. In a large number of burn centers complementary assessments are made by burn wound blood perfusion measured with Laser Doppler Imaging (LDI) and vital dye based systems. These techniques are based on blood perfusion and do not provide immediate assessment of burn wound severity due to the evolving response to the burn injury. This limitation prevents the use of these techniques during the first 48 hours following injury leading to a delay in optimal management. Preliminary in vivo results obtained by our group in rats suggest that hydration imaging acquired with THz and NIR illumination may provide information to non-invasively assess burn severity within the first few hours of injury. This information may allow the burn surgeon to rapidly differentiate between partial and full thickness injury. THz and NIR illumination are eye safe, have very low photon energy, and are extremely sensitive to hydration changes secondary to edema which is part of the tissue response to thermal injury. This study will begin with the construction of a scanned beam, THz imaging system capable of non-contact, imaging of a 10 cm x 10 cm square area in less than 30 seconds. A companion NIR imaging system tuned to the peak absorption wavelength of water will be developed and used for comparison. We will also provide additional information regarding wound hydration. Year 2 will encompass extensive animal work in rats (N = 20) and the capability of these imaging systems for burn severity diagnosis will be evaluated against clinical and histopathological diagnosis.

描述（由申请人提供）：本提案的目标是开发一种基于太赫兹和近红外照明的组织水合成像系统，以在空间和时间上绘制烧伤创面的水合状态并将这些测量结果与烧伤严重程度相关联。烧伤严重程度通常通过外科医生的视觉和触觉反馈来评估，并且根据临床表现和外科医生的经验，诊断准确性差异很大。在许多烧伤中心，通过使用激光多普勒成像 (LDI) 和基于活体染料的系统测量烧伤创面血液灌注来进行补充评估。这些技术基于血液灌注，由于对烧伤的反应不断变化，因此不能立即评估烧伤创面的严重程度。这一限制导致受伤后 48 小时内无法使用这些技术，从而导致最佳治疗的延迟。我们小组在大鼠身上获得的初步体内结果表明，使用太赫兹和近红外照明获得的水合成像可以提供信息，以在受伤的最初几个小时内非侵入性地评估烧伤严重程度。该信息可以使烧伤外科医生快速区分部分损伤和全层损伤。太赫兹和近红外照明对人眼安全，光子能量非常低，并且对继发于水肿的水合作用变化极其敏感，而水肿是组织对热损伤反应的一部分。这项研究将从构建扫描光束太赫兹成像系统开始，该系统能够在 30 秒内对 10 厘米 x 10 厘米见方的区域进行非接触式成像。将开发一个针对水的峰值吸收波长的配套近红外成像系统并用于比较。我们还将提供有关伤口水化的更多信息。第 2 年将包括对大鼠（N = 20）进行广泛的动物研究，并将根据临床和组织病理学诊断来评估这些成像系统诊断烧伤严重程度的能力。