Core Facilities for Neurobiology at Brandeis

布兰迪斯神经生物学核心设施

基本信息

批准号：
8387998
负责人：
MICHAEL ROSBASH
金额：
$ 75.56万
依托单位：
BRANDEIS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-09-30 至 2014-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8387998
关键词：
Address Alzheimer&apos s Disease Amyotrophic Lateral Sclerosis Autistic Disorder Behavior Biological Models Brain Cell physiology Communities Computational Biology Core Facility Disease Electron Microscopy Ensure Epilepsy Faculty Funding Gene Transfer Techniques Genomics High Performance Computing Image Invertebrates Lentivirus Vector Light Mental disorders Moods National Institute of Neurological Disorders and Stroke Neurobiology Neurodegenerative Disorders Neurodevelopmental Disorder Neurons Neurosciences Proteomics Research Project Grants Rett Syndrome Schizophrenia Science Short-Term Memory Sleep disturbances Synaptic Transmission Transfection Transgenic Animals Transgenic Mice Transgenic Mouse Facility Universities Viral fluorescence imaging interest light microscopy member nervous system disorder neural circuit relating to nervous system simulation ultra high resolution

项目摘要

This is a proposal to continue NINDS funding of a Core Facility at Brandeis University. This will allow us to fully exploit the three subcomponents that are now firmly established: a microarray and FACS facility, an imaging facility and a mouse/transgenic facility. They are the underpinnings of a large number of NINDS- funded and other neuroscience-relevant research projects on campus. We also propose major expansions of all three existing cores and the establishment of a fourth core. The microarray/FACS facility will be expanded to encompass proteomics thereby becoming the Genomics/Proteomics Core Facility. The imaging facility will be expanded to include Correlated Light and Electron Microscopy (CLEM) and ultra high- resolution cryo-fluorescence imaging thereby become the Imaging/CLEM Core Facility. The mouse/transgenic facility has recently added the capacity to produce Lentiviral vectors for transfection and transgenesis, and is now referred to as the Transgenic Mouse and Viral Transfection Core Facility. Finally, we will establish a new Computational Core Facility to support large scale neural simulations and computational biology projects as part of a large high-performance computing cluster. These additions will allow the Brandeis community to remain at the cutting edge technologically, and ensure that we can continue to generate exciting and ground-breaking new science. The projects supported by the cores are joined together through the shared interests of multiple neuroscience faculty members in basic as well disease- related aspects of brain and neuron function: cell identity, synaptic transmission and circuits, plasticity, behavior and its modulation. The proposed studies will exploit vertebrate and invertebrate model systems, with a strong emphasis on transgenic animals. They address basic and applied problems that are pertinent to a wide range of neurological and psychiatric diseases including disturbances of excitability, such as epilepsy, disturbances of sleep, waking and mood, neurodevelopmental disorders such as Autism and Rett Syndrome, neurodegenerative disorders, like Amyotrophic Lateral Sclerosis, and disturbances of long and short -term memory such as those that accompany Alzheimer's Disease and Schizophrenia.

这是一项提议，旨在继续在布兰代斯大学（Brandeis University）为核心设施提供资金。这将使我们能够充分利用现在牢固确定的三个子组件：微阵列和FACS设施，成像设施和鼠标/转基因设施。它们是校园内大量NINDS资助和其他与神经科学相关的研究项目的基础。我们还提出了所有三个现有核心的重大扩展，并建立了第四个核心。微阵列/FACS设施将扩展到包含蛋白质组学，从而成为基因组/蛋白质组学核心设施。成像设施将扩展到包括相关的光和电子显微镜（CLEM）和超高分辨率的冷冻荧光成像，从而成为成像/CLEM核心设施。小鼠/转基因设施最近增加了产生转染和转基因的慢病毒载体的能力，现在被称为转基因小鼠和病毒转染核心设施。最后，作为大型高性能计算集群的一部分，我们将建立一个新的计算核心设施，以支持大规模的神经模拟和计算生物学项目。这些增加将使布兰代斯社区能够在技术上保持尖端，并确保我们能够继续产生令人兴奋和开创性的新科学。核心支持的项目通过多个神经科学教师在基本以及疾病与神经元功能相关的方面的共同利益结合在一起：细胞身份，突触传播和电路，可塑性，行为及其调节。拟议的研究将利用脊椎动物和无脊椎动物模型系统，并强调转基因动物。它们解决了与多种神经系统和精神病有关的基本和应用问题阿尔茨海默氏病和精神分裂症。

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Unique transposon landscapes are pervasive across Drosophila melanogaster genomes.

DOI：
10.1093/nar/gkv1193
发表时间：
2015-12-15
期刊：
Nucleic acids research
影响因子：
14.9
作者：
Rahman R;Chirn GW;Kanodia A;Sytnikova YA;Brembs B;Bergman CM;Lau NC
通讯作者：
Lau NC

p75 and TrkA signaling regulates sympathetic neuronal firing patterns via differential modulation of voltage-gated currents.

DOI：
10.1523/jneurosci.3503-08.2009
发表时间：
2009-04-29
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
0
作者：
Luther JA;Birren SJ
通讯作者：
Birren SJ

Conserved piRNA Expression from a Distinct Set of piRNA Cluster Loci in Eutherian Mammals.

DOI：
10.1371/journal.pgen.1005652
发表时间：
2015-11
期刊：
PLoS genetics
影响因子：
4.5
作者：
Chirn GW;Rahman R;Sytnikova YA;Matts JA;Zeng M;Gerlach D;Yu M;Berger B;Naramura M;Kile BT;Lau NC
通讯作者：
Lau NC

CaMKI-dependent regulation of sensory gene expression mediates experience-dependent plasticity in the operating range of a thermosensory neuron.

DOI：
10.1016/j.neuron.2014.10.046
发表时间：
2014-12-03
期刊：
NEURON
影响因子：
16.2
作者：
Yu, Yanxun V.;Bell, Harold W.;Glauser, Dominique A.;Van Hooser, Stephen D.;Goodman, Miriam B.;Sengupta, Piali
通讯作者：
Sengupta, Piali

The capacity of target silencing by Drosophila PIWI and piRNAs.