GENE FUNCTION AND MOLECULAR TOOL KIT

基因功能和分子工具包

• 批准号: 8167791
• 负责人: MICHAEL J KERN
• 金额: $ 27.85万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167791
• 关键词: Adult; Area; Cardiovascular system; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Faculty; Funding; Gene Targeting; Goals; Grant; Heart Diseases; Human Resources; Institution; Mentors; Molecular; Mus; Plasmids; Research; Research Personnel; Research Support; Resources; Services; Source; South Carolina; Techniques; Technology; Testing; Training; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; base; beneficiary; cost; gene function; malformation; member; programs; success; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of the Gene Function core is to provide intellectual and physical resources for testing Gene Function to understand cardiovascular malformations and adult cardiac disease using transgenic and gene targeted mice. The COBRE trainees will be the primary beneficiaries and users of this facility on a cost free basis and with enhanced priority. However this core also will support the research programs of faculty members at MUSC, and throughout the state of South Carolina. Toward this goal the core has the following three main services (1) generate transgenic mice, (2) generate gene targeted mice, (3) mentor the COBRE trainees in utilizing these techniques for cardiovascular research. There are two additional areas (plasmid construction and related mouse services) which support the main services described above. Without this core facility the research tools and technology of transgenic and gene targeted mice, would be out of reach of most junior faculty. For them to invest in the training, or cost, to establish the technical resources and expertise, would come at the expense of focusing on their aims. This core will alleviate these issues and facilitate the analysis of Gene Function by the COBRE trainees and others at MUSC and the State of South Carolina. The Gene Function Core financially supports some of the percent effort of directors and technical personnel for both the MUSC Transgenic Facility and MUSC Gene Targeting Facility. These two facilities are critical to the success of this COBRE and they are the only facilities of their kind in the entire state of South Carolina, thereby the COBRE support enhances the success of additional research programs at MUSC and the state.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 基因功能核心的目的是为使用转基因和基因靶向小鼠测试基因功能提供智力和体力资源，以了解心血管畸形和成人心脏病。 COBRE 学员将成为该设施的主要受益者和用户，并且免费且优先。 然而，该核心也将支持 MUSC 和整个南卡罗来纳州教员的研究项目。 为了实现这一目标，核心提供以下三个主要服务（1）生成转基因小鼠，（2）生成基因靶向小鼠，（3）指导 COBRE 学员利用这些技术进行心血管研究。 还有两个其他领域（质粒构建和相关的小鼠服务）支持上述主要服务。 如果没有这个核心设施，大多数初级教师将无法获得转基因和基因靶向小鼠的研究工具和技术。 对于他们来说，投资培训或成本来建立技术资源和专业知识，将以牺牲对目标的关注为代价。 该核心将缓解这些问题，并促进 COBRE 学员以及 MUSC 和南卡罗来纳州的其他人员对基因功能进行分析。 基因功能核心为 MUSC 转基因设施和 MUSC 基因打靶设施的董事和技术人员的部分工作提供了财政支持。 这两个设施对于 COBRE 的成功至关重要，它们是整个南卡罗来纳州唯一的此类设施，因此 COBRE 的支持增强了 MUSC 和该州其他研究项目的成功。