IMAGING CORE

成像核心

• 批准号: 8167963
• 负责人: KAREN E MARTIN
• 金额: $ 19.27万
• 依托单位: WEST VIRGINIA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167963
• 关键词: Animal Model; Animals; Bioluminescence; Calcium; Cells; Color; Computer Retrieval of Information on Scientific Projects Database; Computer software; Computers and Advanced Instrumentation; Cryopreservation; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Genetic; Grant; Histology; Image; Institution; Lasers; Life; Microdissection; Microinjections; Microscope; Monitor; Mus; Neurons; Operative Surgical Procedures; Records; Reporter; Research; Research Personnel; Resources; Scanning; Services; Source; Speed; System; Time; Tissue Banks; United States National Institutes of Health; Universities; cellular imaging; in vivo; light microscopy; reconstruction; research study; sperm cell

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Imaging Facility provides advanced instrumentation and expertise to support imaging projects at the university. The facility has two branches that specialize in light microscopy and small animal imaging. The Animal Models and Imaging Facility currently performs longitudinal bioluminescence and fluorescence studies on mice using the Xenogen IVIS Lumina. This system allows researchers to perform real-time, in vivo imaging to monitor and record cellular and genetic activity within a small animal using fluorescent and/or bioluminescent reporters. In addition, this facility also offers services such as mouse colony management, tissue collection, cryopreservation of sperm and various surgeries. The Microscope Imaging Facility provides light microscopy image acquisition and analysis services. The facility currently has two Zeiss LSM510 laser scanning confocal systems for experiments such as 3D rendering and FRAP. An Olympus epifluorescent microscope equipped with the MicroBrightField Neurolucida and Stereo Investigator software packages is used for color histology records, 3D neuron reconstruction and serial section reconstruction. A Zeiss PALM MicroBeam system is available for non-contact microdissection of single cells or groups of cells. For live-cell imaging, the facility has a Nikon epifluorescent microscope for multi-color time-lapse, FRET and calcium ratio imaging. This microscope also has an Eppendorf FemtoJet microinjection system. For high-end live-cell imaging, the facility has a Nikon Swept-Field for high-speed, multi-color confocal imaging. This system is also equipped with a laser TIRF illuminator and appropriate hardware for widefield fluorescent and brightfield imaging.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 成像设施提供先进的仪器和专业知识来支持大学的成像项目。 该设施有两个专门从事光学显微镜和小动物成像的分支机构。 动物模型和成像设施目前使用 Xenogen IVIS Lumina 对小鼠进行纵向生物发光和荧光研究。 该系统允许研究人员使用荧光和/或生物发光报告器进行实时体内成像，以监测和记录小动物内的细胞和遗传活动。 此外，该设施还提供小鼠群体管理、组织采集、精子冷冻保存和各种手术等服务。 显微镜成像设施提供光学显微镜图像采集和分析服务。 该设施目前拥有两台 Zeiss LSM510 激光扫描共焦系统，用于 3D 渲染和 FRAP 等实验。 配备 MicroBrightField Neurolucida 和 Stereo Investigator 软件包的奥林巴斯落射荧光显微镜用于彩色组织学记录、3D 神经元重建和连续切片重建。 Zeiss PALM MicroBeam 系统可用于单个细胞或细胞组的非接触式显微切割。 对于活细胞成像，该设施拥有尼康落射荧光显微镜，用于多色延时、FRET 和钙比率成像。 该显微镜还配有 Eppendorf FemtoJet 显微注射系统。 对于高端活细胞成像，该设施拥有尼康扫描场，用于高速、多色共焦成像。 该系统还配备了激光 TIRF 照明器和用于宽场荧光和明场成像的适当硬件。