The Functional Organization of Inhibition in the Olfactory Bulb In Vivo

体内嗅球抑制的功能组织

基本信息

批准号：
8573250
负责人：
Michael Nicholas Economo
金额：
$ 3万
依托单位：
UNIVERSITY OF UTAH
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-07-01 至 2014-01-07
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): In the olfactory bulb, a single synapse separates afferent sensory input and the principal neurons that project to higher cortical areas. Nevertheless, the vast majority of cells and connections in this structure are devoted to two distinct inhibitory circuits: one located superficially in the glomerular layer and one involving granule cells in infraglomerular layers. Soon after they were initially described, it was suggested that these inhibitory circuits might provide center-surround inhibition in an analogous fashion to other primary sensory areas. Nevertheless, the manner in which these circuits are activated by olfactory stimuli in vivo has yet to be demonstrated, and their importance in shaping olfactory bulb output remains controversial. In this proposal, the spread of inhibition laterally from the sites of olfactory receptor neuron input through the glomerular layer will be directly visualized t determine how lateral inhibition at this level might contribute to olfactory information processing In addition, the postsynaptic effect of inhibition arising from both lateral circuits will be assesed in identified olfactory bulb output neurons. These experiments will provide crucial insights into the role of lateral inhibitory circuits in vivo, and, by determining the differential effects of thse circuits on the excitability of different classes of output neurons, a better understanding of parallel pathways projecting to higher brain structures.

描述（由申请人提供）：在嗅球中，单个突触将传入感觉输入和投射到更高皮质区域的主要神经元分开。然而，该结构中的绝大多数细胞和连接致力于两种不同的抑制回路：一种位于肾小球层表面，另一种涉及肾小球下层的颗粒细胞。在最初描述它们后不久，有人建议这些抑制回路可能以与其他主要感觉区域类似的方式提供中心-周围抑制。然而，这些回路在体内被嗅觉刺激激活的方式尚未得到证实，并且它们在塑造嗅球输出方面的重要性仍然存在争议。在这个提议中，抑制从嗅觉受体神经元输入通过肾小球层的位点横向扩散将被直接可视化，以确定该水平的横向抑制如何有助于嗅觉信息处理此外，抑制的突触后效应来自于两者将在已识别的嗅球输出神经元中评估侧向回路。这些实验将为体内侧抑制回路的作用提供重要的见解，并且通过确定这些回路对不同类别输出神经元兴奋性的差异影响，更好地理解投射到更高大脑结构的平行通路。