A chemical-genetic approach to decipher the function of corticothalamic feedback

破译皮质丘脑反馈功能的化学遗传学方法

• 批准号: 8512439
• 负责人: Daniel B. Polley
• 金额: $ 24.4万
• 依托单位: MASSACHUSETTS EYE AND EAR INFIRMARY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-01 至 2015-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8512439
• 关键词: Acoustics; Action Potentials; Affect; Animals; Area; Auditory; Auditory area; Axon; Behavior; Behavioral; Blood - brain barrier anatomy; Brain; Cerebral cortex; Cholinergic Receptors; Chronic; Cortical Column; Data; Designer Drugs; Dorsal; Dose; Dreams; Drug Receptors; Engineering; Epilepsy; Feedback; Frequencies; Hearing; Hour; Implant; Individual; Injection of therapeutic agent; Lead; Learning; Lesion; Link; Literature; Measures; Medial; Medial geniculate body; Mediating; Medical Technology; Membrane; Memory; Morphology; Mus; Neuraxis; Neurons; Parkinson Disease; Pathway interactions; Perception; Pharmaceutical Preparations; Phase; Physiology; Presynaptic Terminals; Process; Reporting; Research; Role; Sensory; Slice; Specificity; Stream; Synapses; System; Techniques; Technology; Testing; Thalamic structure; Time; Tinnitus; Transgenic Mice; Unconscious State; Viral Vector; Work; auditory stimulus; awake; base; cell type; chemical genetics; cognitive function; corticofugal system; design; dosage; extracellular; in vivo; insight; loss of function; nervous system disorder; next generation; novel strategies; predictive modeling; public health relevance; receptive field; receptor; relating to nervous system; research study; response; sound; synthetic drug; tool; vigilance; vocalization

DESCRIPTION (provided by applicant: Sensory representations are dynamically maintained by ascending and descending connections linking the cerebral cortex and the thalamus. Although the overall extent and topographic specificity of descending corticothalamic projections can equal or surpass that of thalamocortical projections, little is known about their role in perception or learning. Here, we propose a new chemical-genetic approach known as DREADDs (Designer Receptors Exclusively Activated by Designer Drugs) to parse the functional contribution of corticothalamic circuits that arise from layer 5 versus layer 6 of primay and secondary areas of the auditory cortex. By silencing specific cortical feedback circuits while recording from ensembles of thalamic neurons in mice that are either passively listening to sound or are actively engaged in listening tasks, we will gain deeper insight into the workings of multiple, parallel feedback systems that enable the cerebral cortex to modify its afferent input stream.

描述（由申请人提供：通过连接大脑皮层和丘脑的连接的上升和下降连接来动态维护感官表示形式。尽管降级皮质丘脑预测的总体程度和地形特异性相等或超过丘脑皮层预测的丘脑的角色，但在此方面却鲜为人知，但在此方面却鲜为人知。被设计师药物激活），以解析由第5层与听觉皮层的Primay和次要区域的第6层与第6层产生的功能贡献，通过使特定的皮质反馈回路沉默，同时从倾听的倾听或倾听的倾听训练中，以录制的是从事的thalamic Neurons。使大脑皮层可以修改其传入输入流的反馈系统。