750MHz Wide Bore NMR Spectrometer

750MHz 大口径核磁共​​振波谱仪

• 批准号: 8334229
• 负责人: ANGELA M. GRONENBORN
• 金额: $ 200万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2016-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8334229
• 关键词: Address; Area; Biological; Biomedical Research; Delaware; Development; Ensure; Environment; Fostering; Funding; HIV; Hormone Receptor; Housing; Institution; Investigation; Membrane; Membrane Proteins; Methods; Neurodegenerative Disorders; Neurotransmitter Receptor; Nuclear Magnetic Resonance; Pennsylvania; Peptides; Protein Dynamics; Proteins; Protocols documentation; Reaction; Research; Research Infrastructure; Research Personnel; Research Project Grants; Science; Scientist; Spectrum Analysis; Structure; United States National Institutes of Health; Universities; cold temperature; data acquisition; instrument; instrumentation; macromolecular assembly; medical schools; novel; protein aggregate; protein folding; research study; solid state; solid state nuclear magnetic resonance; structural biology; three dimensional structure; tool; trafficking

DESCRIPTION (provided by applicant): This request is for funds to acquire a wide bore 750 MHz nuclear magnetic resonance (NMR) spectrometer for solid-state investigations of membrane proteins, macromolecular assemblies, and protein aggregates. Solid-state NMR (SSNMR) is an essential tool for understanding the structures and dynamics of proteins that are embedded within biological membranes or that form large aggregates and assemblies. An advantage of SSNMR, over other structural methods, is that it does not require proteins to form crystals or be soluble, and can be conducted on protein assemblies greater than 100 kD in size. The proposed acquisition addresses the expanding needs of multiple researchers at the University of Pittsburgh and their collaborators across multiple universities, as the lack of dedicated high-field SSNMR instrumentation in the greater Pittsburgh area is increasingly limiting their research on multiple biomedical fronts. The new instrument will be housed in the state-of-the art NMR facility in the Department of Structural Biology at the University of Pittsburgh - part of a recently established Department of Structural Biology at the School of Medicine. The instrument will serve the needs of scientists in multiple collaborative networks, including the NIH-supported Pittsburgh Center for HIV Protein Interactions (PCHPI). Its incorporation into a pre-existing and otherwise well-equipped research center will ensure optimal usage of this cutting-edge instrument. The major users of the requested instrument will be at the University of Pittsburgh School of Medicine and the University of Delaware, with additional users at Carnegie Mellon University, Bucknell University and elsewhere (12 groups in total). Multiple NIH-funded research projects will benefit from the availability of this dedicated high-field solid-state NMR spectrometer, including studies of proteins and protein assemblies involved in HIV-cellular protein interactions, neurodegenerative disorders, neurotransmitter and hormone receptors, as well as cellular trafficking. The instrument will be fully outfitted for multidimensional spectroscopy in the solid state, specifically for 3D structure determination and dynamics analysis of uniformly, extensively and selectively isotopically (13C, 15N, 2H, 19F) enriched proteins, peptides, and macromolecular assemblies. The instrument will be equipped with low temperature setup and probes, permitting investigations of protein folding and reaction intermediates as well as comprehensive dynamics studies, at enhanced sensitivity. The probes and the dual receiver capabilities will enable development of novel experiments and data acquisition protocols to facilitate studies of large proteins and protein assemblies. This 750 MHz SSNMR instrument will have an immediate impact on the infrastructure at the University of Pittsburgh and participating institutions as it will enable cutting-edge science, will foster highl collaborative biomedical research, and will support the vibrant research environment in the State of Pennsylvania.

描述（由申请人提供）：此请求是为了获得宽孔750 MHz核磁共振（NMR）光谱仪，以对膜蛋白，大分子组件和蛋白质聚集体进行固态研究。固态NMR（SSNMR）是了解嵌入生物膜中或形成大型聚集体和组件的蛋白质的结构和动力学的必要工具。 SSNMR比其他结构方法的优点是，它不需要蛋白质形成晶体或可溶性，并且可以对大于100 kd的蛋白质组件进行进行。拟议的收购解决了匹兹堡大学及其在多所大学的合作者的多个研究人员的不断增长的需求，因为大匹兹堡地区缺乏专用的高场SSNMR仪器，越来越多地将他们的研究限制在多个生物医学方面。新工具将安置在匹兹堡大学结构生物学系的最先进的NMR设施中，这是医学院最近建立的结构生物学系的一部分。该仪器将在多个协作网络中满足科学家的需求，包括NIH支持的匹兹堡HIV蛋白质相互作用中心（PCHPI）。它将其纳入预先存在的和其他设备齐全的研究中心将确保最佳使用该尖端工具。所请求的乐器的主要用户将在匹兹堡大学医学院和特拉华大学，还有卡内基·梅隆大学，巴克内尔大学和其他地方的其他用户（总计12组）。多个NIH资助的研究项目将受益于这种专用的高档固态NMR光谱仪的可用性，包括研究参与HIV细胞蛋白质相互作用，神经退行性疾病，神经递质和激素受体的蛋白质和蛋白质组件的研究，以及以及细胞​​贩运。 该仪器将在固态的多维光谱中完全配备，特别是用于对均匀，广泛，广泛和选择性的同位素（13C，15N，2H，19F）的3D结构测定和动态分析，富集的蛋白质，肽和大分子组件。该仪器将配备低温设置和探针，允许对蛋白质折叠和反应中间体以及全面的动力学研究进行研究。探针和双重接收器功能将使新实验和数据采集方案得以开发，以促进大型蛋白质和蛋白质组件的研究。这种750 MHz SSNMR仪器将对匹兹堡大学的基础设施和参与机构的基础设施产生直接影响，因为它将促进尖端科学，将促进Highl Highl协作生物医学研究，并将支持宾夕法尼亚州的充满活力的研究环境。