Acquisition of an Illumina Hi-Seq 2500

购买 Illumina Hi-Seq 2500

• 批准号: 8447277
• 负责人: STEVEN R. GILL
• 金额: $ 55万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-15 至 2014-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447277
• 关键词: Bioinformatics; Biomedical Research; Biometry; Computational Biology; Consultations; DNA; DNA Sequence; Dentistry; Development; Equipment; Funding; Future; Gene Expression Profile; Generations; Genome; Informatics; Libraries; Minor; Nucleic Acids; Preparation; Proteins; Proteomics; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resource Sharing; Resources; Running; Sampling; Services; Structure; System; Time; United States National Institutes of Health; Universities; base; cost; cost effective; exome sequencing; experience; functional genomics; instrument; medical schools; meetings; member; metabolomics; next generation

DESCRIPTION (provided by applicant): Development of a new generation of high-throughput DNA sequencers has led to a transformation of biomedical research. These instruments provide a wide range of applications on a whole genome scale, including SNP discovery, transcriptome profiling, targeted exome sequencing and identification of protein- nucleic acid interactions. This proposal seeks funds to expand high-throughput sequencing capacity of the University of Rochester Functional Genomics Center (FGC) by addition of an Illumina HiSeqTM 2500 System next-generation DNA sequencer. Existing next-generation capabilities within the FGC are supported by a single Illumina GAIIxTM instrument. Replacement of the GAIIxTM with the new HiSeqTM 2500 would substantially expand capacity of the FGC to meet the increasing demand for high-throughput sequencing services at our two campuses, the University of Rochester School of Medicine and Dentistry (SMD) and the University of Rochester main campus (UR). As a result of a significantly larger throughput and a faster run time, access to the HiSeqTM 2500 instrument will save investigators time and cost on their research projects. This instrument will primarily be used by a group of 10 Major and 8 Minor Users who are members of an existing strong Illumina user base with a diversity of NIH funded projects. Because the HiSeqTM 2500 will replace the existing GAIIxTM in the FGC, it will be easily integrated into an established workflow without the need for additional ancillary equipment, staff or computing infrastructure. The FGC is part of an existing multi-core structure providing shared resources to SMD investigators, including DNA sequencing, microarrays, proteomics, metabolomics as well as informatics and statistical support. Support provided by the FGC for high-throughput sequencing includes initial consultation, sample preparation, library construction and sequencing. Bioinformatics and statistical support is provided through the FGC by services integrated with the Center for Integrated Research Computing (CIRC) and The Department of Biostatistics and Computational Biology. These combined resources and support will allow experienced investigators as well as investigators unfamiliar with high-throughput sequencing to take immediate advantage of the HiSeqTM 2500 capabilities. Addition of the HiSeqTM 2500 as a cost effective shared resource will provide a substantial benefit to multiple NIH funded projects and stimulate additional projects among new investigators, enabling them develop future NIH funded projects and compete at the highest scientific level.

描述（由申请人提供）： 新一代高通量DNA测序仪的发展导致了生物医学研究的转变。这些仪器在整个基因组量表上提供了广泛的应用，包括SNP发现，转录组分析，有针对性的外显子组测序以及蛋白质核酸相互作用的鉴定。该提案寻求资金来扩大罗切斯特大学功能基因组学中心（FGC）的高通量测序能力，并添加Illumina hiseqtm 2500 System下一代DNA Sequencer。 FGC中现有的下一代功能由单个Illumina Gaiixtm仪器支持。用新的HISEQTM 2500取代GaiixTM将大大扩大FGC的能力，以满足我们在我们两个校园，罗切斯特医学院和牙科大学（SMD）和罗切斯特大学主校区（UR）（UR）的两个校园中对高通量测序服务的日益增长的需求。由于吞吐量和更快的运行时间，访问HISEQTM 2500仪器将为调查人员节省其研究项目的时间和成本。该工具将主要由10个主要和8个未成年人的小组使用，这些用户是现有强大的Illumina用户群的成员，拥有多种NIH资助的项目。由于HISEQTM 2500将替换FGC中现有的GaiixTM，因此它将很容易集成到已建立的工作流程中，而无需其他辅助设备，人员或计算基础架构。 FGC是现有多核结构的一部分，该结构为SMD研究人员提供共享资源，包括DNA测序，微阵列，蛋白质组学，代谢组学以及信息学和统计支持。 FGC为高通量测序提供的支持包括初始咨询，样本准备，图书馆构造和测序。生物信息学和统计支持是通过FGC通过与综合研究计算中心（CIRC）和生物统计学和计算生物学系集成的服务提供的。这些合并的资源和支持将使经验丰富的研究人员以及不熟悉高通量测序的调查人员可以立即利用Hiseqtm 2500功能。 HISEQTM 2500作为具有成本效益的共享资源将为多个NIH资助的项目带来可观的好处，并激发新研究人员之间的其他项目，从而使他们能够开发未来的NIH资助项目并在最高科学层面竞争。