LTQ Velos Pro mass spectrometer with ETD and other options

具有 ETD 和其他选件的 LTQ Velos Pro 质谱仪

• 批准号: 8447950
• 负责人: Paul D Gershon
• 金额: $ 37.41万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447950
• 关键词: Aging; Area; Biological Sciences; Biomedical Research; Blood Vessels; Brain; Chlamydia Infections; Clinical; Committee Members; Communicable Diseases; Complex; Embryonic Development; Enzymes; Funding; Global Change; Growth Factor; Health; Homeostasis; Human; Infection; Investigation; Ions; Mass Spectrum Analysis; Messenger RNA; Midgut; Modification; Molecular; N.I.H. Research Support; Nitric Oxide Synthase; Poliomyelitis; Proteins; Proteome; RNA Splicing; Research; Rhinovirus; Salivary Glands; Schools; Screening for Prostate Cancer; Signal Transduction; Time; United States National Institutes of Health; Vaccinia virus; base; experience; instrument; mass spectrometer; medical schools; member; molecular dynamics; programs; proto-oncogene protein pim-1; research study; response; urinary; vector mosquito

DESCRIPTION (provided by applicant): This proposal seeks funding for the purchase of an LTQ Velos Pro mass spectrometer from ThermoFisher, with ETD option and nanoLC. The combination of fast cycle times, high sensitivity and multiple ion fragmentation modes provided by this instrument is not available to the major user group for this proposal, yet would allow fundamental enhancements in quantitative proteome- and quantitative phosphoproteome-based mass spectrometry experiments in support of a number of NIH funded research programs at UCI. Specific projects that will be enhanced include investigation of the molecular mechanisms underlying the modulation of cell signaling upon infection by vaccinia virus (Dr. Gershon); global cellular responses to polio and rhinovirus (Dr. Semler) and Chlamydia infection (Dr. DeLa Maza), understanding the salivary gland and midgut responses of mosquito vectors to infectious diseases (Dr. James), early detection of prostate cancer markers in the urinary proteome (Dr. Mercola), global changes in phosphosite occupancy as a result of Pim-1 kinase over-expression and specific inhibition (Dr. Lilly), finding growth factors involved in blood vessel branching durig embryogenesis and their homeostasis (Dr. Hughes), plasticity of modifications incurred during the assembly of human mRNA splicing complexes (Dr. Hertel), understanding responses to protein aging in the brain, and molecular dynamics of the key regulatory enzyme nitric oxide synthase (Dr. Poulos). The P.I. of this proposal, with 12 years experience in protein mass spectrometry, will administer the instrument in a shared area with the blessing of the aforementioned collaborators under the oversight of a supervisory committee with external members including Dr. Joe Loo (UCLA) and Dr. Julian Whitelegge (UCLA), and internal committee members representing both basic and clinical programs. 21 NIH-supported research programs related to human health spanning six departments in two schools of UCI are expected to benefit significantly from the capabilities of the proposed instrument. The requested instrument would represent a significant advance in interdisciplinary biomedical research capabilities within the UCI Schools of Medicine and Biological Sciences.

描述（由申请人提供）： 该提案寻求资助从Thermofisher购买LTQ Velos Pro质谱仪，并带有ETD选项和Nanolc。该仪器提供的快速周期时间，高灵敏度和多个离子碎片模式的组合是该提案的主要用户组不可用的，但将允许基于定量蛋白质组和基于定量磷光蛋白质组的质谱实验的基本增强，以支持UCI的许多NIH资助研究计划。将会增强的特定项目包括研究牛仔植物病毒感染后细胞信号传导调节的分子机制（Gershon博士）；全球细胞对脊髓灰质炎和鼻病毒（Semler博士）和衣原体感染（Dela Maza博士）的反应，了解蚊子对感染性疾病的唾液腺和中肠反应（詹姆斯博士）（詹姆斯博士），早期发现泌尿药物中的前列腺癌特定于Prostate cansie temerty oferary proseme（P. Mercola），占整体占用的占用量，占整体次数的占用。抑制作用（Lilly博士），发现与血管分支的生长因子杜里格胚胎发生及其稳态（Hughes博士），在人类mRNA剪接复合物组装过程中发生的修饰的可塑性（Hertel博士），了解对大脑中蛋白质衰变的反应，以及密钥调节型氧化氧化物的蛋白质的反应，以及密钥调节型氧化物。 P.I.在蛋白质质谱学方面拥有12年经验的这项建议，将在共享区域内管理该乐器，并在监督委员会的监督下与外部成员的监督下，包括Joe Loo（UCLA）博士（UCLA）和Julian Whitelegge博士（UCLA），以及代表基本和临床计划的内部委员会成员。 21个与人类健康有关的NIH支持的研究计划，跨越UCI学校的六个部门，预计将从拟议的工具的能力中受益匪浅。所请求的工具将代表UCI医学和生物科学学校内的跨学科生物医学研究能力的重大进步。