PROTEIN MASS SPECTROMETRY (SHARED RESOURCE)

蛋白质质谱（共享资源）

• 批准号: 7944552
• 负责人: Paul D Gershon
• 金额: $ 2.14万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-06 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944552
• 关键词: Amides; Arts; Binding Sites; Biological Assay; Biological Markers; Cancer Center; Cancer Center Support Grant; Cell Extracts; Complex Mixtures; Computer software; Data; Deuterium; Development; Dimensions; Fractionation; Funding; Future; Gel; High Pressure Liquid Chromatography; Hydrogen; Individual; Ions; Isotopes; Label; Ligand Binding; Mass Spectrum Analysis; Measures; Methods; Peptides; Phosphopeptides; Phosphorylated Peptide; Property; Proteins; Proteolysis; Proteomics; Protocols documentation; Protons; Pump; Reagent; Relative (related person); Resolution; Resource Sharing; Robotics; Running; Sampling; Scheme; Services; Shotguns; Slice; Solvents; Spottings; System; Techniques; University of California Irvine Cancer Center; Virion; Work; anticancer research; base; frontier; in vivo; instrument; mass analyzer; mass spectrometer; mass spectrometry shared resource; nano-electrospray; protein complex; tool; two-dimensional

Protein identification and proteomics are important new tools in cancer research. The Protein Mass Spectrometry Shared Resource will provide high-level mass spectrometry of biomolecules and protein analyses. This Shared Resource is based around a pair of tandem mass analyzers (the AB 4700 MALDITOF/ TOF and Thermo LTQ nanoESI-QIT), with access to as many as ten HPLC systems containing 10 quaternary and two binary pumps, seven PCs running at least 21 software packages, and numerous smaller items. The Facility is able to routinely identify proteins in slices from polyacryamide gels. In addition, it can routinely identify proteins in pull-down mixtures, complex virions, and whole cell extracts. They have done multi-dimensional (up to three dimensions) of tandem HPLC prior to LC-MALDI analysis. This capability includes two-dimensional protein fractionation (PF2D) and two and three dimensions of tandem peptide fractionation. This Facility provides an exciting springboard to the frontiers of state-of-the art proteomics. The Protein Mass Spectrometry Facility has been supported by the Cancer Center as a developing Shared Fesource for the past three years. Permanent funds are now requested for support as a full Shared Resource.

蛋白质鉴定和蛋白质组学是癌症研究中的重要新工具。蛋白质质量 光谱共享资源将提供生物分子和蛋白质的高级质谱 分析。该共享资源基于一对串联质量分析仪（AB 4700 MALDITOF/ TOF和Thermo LTQ纳米QIT），可访问多达10个HPLC系统，其中10个 第四纪和两个二进制泵，七台运行至少21个软件包的PC以及许多较小的PC 项目。该设施能够常规地从聚丙烯酰胺凝胶中鉴定在切片中的蛋白质。另外，它可以 通常在下拉混合物，复杂的病毒体和全细胞提取物中识别蛋白质。他们做到了 在LC-MALDI分析之前，串联HPLC的多维（最多三维）。这个功能 包括二维蛋白分馏（PF2D）和串联肽的两个和三个维度 分馏。该设施为最先进的蛋白质组学前沿提供了令人兴奋的跳板。 蛋白质质谱设施已得到癌症中心的支持 在过去三年中。现在要求永久资金作为完整共享的支持 资源。